ID   AI5B_YEAST              Reviewed;         354 AA.
AC   Q9ZZX0; A0A0A7NYD5;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Intron-encoded DNA endonuclease aI5 beta;
DE            EC=3.1.-.-;
GN   Name=AI5_BETA; OrderedLocusNames=Q0075;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=9872396; DOI=10.1016/s0014-5793(98)01467-7;
RA   Foury F., Roganti T., Lecrenier N., Purnelle B.;
RT   "The complete sequence of the mitochondrial genome of Saccharomyces
RT   cerevisiae.";
RL   FEBS Lett. 440:325-331(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   SPLICING.
RX   PubMed=2842150; DOI=10.1002/j.1460-2075.1988.tb02963.x;
RA   Seraphin B., Simon M., Faye G.;
RT   "MSS18, a yeast nuclear gene involved in the splicing of intron aI5 beta of
RT   the mitochondrial cox1 transcript.";
RL   EMBO J. 7:1455-1464(1988).
RN   [4]
RP   SPLICING.
RX   PubMed=2555177; DOI=10.1002/j.1460-2075.1989.tb08569.x;
RA   Valencik M.L., Kloeckener-Gruissem B., Poyton R.O., McEwen J.E.;
RT   "Disruption of the yeast nuclear PET54 gene blocks excision of
RT   mitochondrial intron aI5 beta from pre-mRNA for cytochrome c oxidase
RT   subunit I.";
RL   EMBO J. 8:3899-3904(1989).
RN   [5]
RP   SPLICING.
RX   PubMed=1699677; DOI=10.1007/bf00312599;
RA   Bousquet I., Dujardin G., Poyton R.O., Slonimski P.P.;
RT   "Two group I mitochondrial introns in the cob-box and coxI genes require
RT   the same MRS1/PET157 nuclear gene product for splicing.";
RL   Curr. Genet. 18:117-124(1990).
RN   [6]
RP   SPLICING.
RX   PubMed=2158076; DOI=10.1093/nar/18.6.1369;
RA   Conrad-Webb H., Perlman P.S., Zhu H., Butow R.A.;
RT   "The nuclear SUV3-1 mutation affects a variety of post-transcriptional
RT   processes in yeast mitochondria.";
RL   Nucleic Acids Res. 18:1369-1376(1990).
CC   -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- MISCELLANEOUS: Endonuclease aI5 beta (group I intron) is encoded within
CC       an intron of the mitochondrial COX1 gene. Splicing from COX1 requires
CC       PET54, MRS1, SUV3 and MSS18. Translational initiation codon is
CC       predicted to be ATA rather than ATG.
CC   -!- SIMILARITY: Belongs to the LAGLIDADG endonuclease family.
CC       {ECO:0000255}.
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DR   EMBL; KP263414; AIZ98887.1; -; Genomic_DNA.
DR   PIR; S78652; S78652.
DR   RefSeq; NP_009311.2; NC_001224.1.
DR   AlphaFoldDB; Q9ZZX0; -.
DR   SMR; Q9ZZX0; -.
DR   BioGRID; 34791; 1.
DR   STRING; 4932.Q0075; -.
DR   PaxDb; Q9ZZX0; -.
DR   PRIDE; Q9ZZX0; -.
DR   EnsemblFungi; Q0075_mRNA; Q0075; Q0075.
DR   GeneID; 854599; -.
DR   KEGG; sce:Q0075; -.
DR   SGD; S000007266; AI5_BETA.
DR   VEuPathDB; FungiDB:Q0075; -.
DR   HOGENOM; CLU_080623_0_0_1; -.
DR   InParanoid; Q9ZZX0; -.
DR   BioCyc; YEAST:G3O-34377-MON; -.
DR   PRO; PR:Q9ZZX0; -.
DR   Proteomes; UP000002311; Mitochondrion.
DR   RNAct; Q9ZZX0; protein.
DR   GO; GO:0005739; C:mitochondrion; TAS:SGD.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.28.10; -; 2.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   Pfam; PF00961; LAGLIDADG_1; 1.
DR   SUPFAM; SSF55608; SSF55608; 2.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Intron homing; Mitochondrion; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..354
FT                   /note="Intron-encoded DNA endonuclease aI5 beta"
FT                   /id="PRO_0000270561"
SQ   SEQUENCE   354 AA;  42279 MW;  FA9175E5748AD798 CRC64;
     MLMLLMMILT NNKVFMETLY YYLMFNFQLM SPFGVPVPGP APETKDIKNL YESIMNNYIN
     ILNKYTININ KDNINKLKFL DNYTEEEKGY YLSGLFEGDG NIYTRCFSIT FSLEDVLLAN
     YLCTYFKIGH ITAKYNFNKE LTAVKWNIMK KKEQEVFMNY INGKLLTYKR YDQYFKYNFN
     NRLNIKLLKP KEFDLTLNPW LTGFNDADGY FYTGFQKHKN SQWLKFHLEL SQKDSYILDI
     IKKYFKTGGI LKRDYKSGAT AYIYKAQSSK AMKPFIEYFN NYQPLSTRRY KQYLLLNIAY
     LLKLNKLHML TNSLLMLKEL MLLQSVKNMS LEMKNELNNR VKIIINKTHY NNIE