FABPL_CHICK
ID FABPL_CHICK Reviewed; 126 AA.
AC P80226; Q90WB0;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Fatty acid-binding protein, liver;
DE AltName: Full=Fatty acid-binding protein 1;
DE AltName: Full=Liver basic FABP;
DE Short=LB-FABP;
DE AltName: Full=Liver bile acid-binding protein;
DE Short=L-BABP;
DE AltName: Full=Liver-type fatty acid-binding protein;
DE Short=L-FABP;
GN Name=FABP1;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=White leghorn; TISSUE=Liver;
RA Murai A., Kusumoto K., Okumura J.;
RT "Regulation of gene expression of two liver type fatty acid binding
RT proteins in chicken.";
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PROTEIN SEQUENCE OF 2-126, AND ACETYLATION AT ALA-2.
RC TISSUE=Liver;
RX PubMed=7553344; DOI=10.1016/0305-0491(94)90010-8;
RA Ceciliani F., Monaco H.L., Ronchi S., Faotto L., Spadon P.;
RT "The primary structure of a basic (pI 9.0) fatty acid-binding protein from
RT liver of Gallus domesticus.";
RL Comp. Biochem. Physiol. 109B:261-271(1994).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
RX PubMed=2266974; DOI=10.1007/bf00231372;
RA Scapin G., Spadon P., Mammi M., Zanotti G., Monaco H.L.;
RT "Crystal structure of chicken liver basic fatty acid-binding protein at 2.7
RT A resolution.";
RL Mol. Cell. Biochem. 98:95-99(1990).
RN [4]
RP STRUCTURE BY NMR.
RX PubMed=12652125; DOI=10.1023/a:1022277727303;
RA Vasile F., Ragona L., Catalano M., Zetta L., Perduca M., Monaco H.L.,
RA Molinari H.;
RT "Solution structure of chicken liver basic fatty acid binding protein.";
RL J. Biomol. NMR 25:157-160(2003).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH CHOLATE.
RX PubMed=15518556; DOI=10.1021/bi0489661;
RA Nichesola D., Perduca M., Capaldi S., Carrizo M.E., Righetti P.G.,
RA Monaco H.L.;
RT "Crystal structure of chicken liver basic fatty acid-binding protein
RT complexed with cholic acid.";
RL Biochemistry 43:14072-14079(2004).
RN [6]
RP STRUCTURE BY NMR.
RX PubMed=16439356; DOI=10.1074/jbc.m513003200;
RA Ragona L., Catalano M., Luppi M., Cicero D., Eliseo T., Foote J.,
RA Fogolari F., Zetta L., Molinari H.;
RT "NMR dynamic studies suggest that allosteric activation regulates ligand
RT binding in chicken liver bile acid-binding protein.";
RL J. Biol. Chem. 281:9697-9709(2006).
CC -!- FUNCTION: Binds free fatty acids and their coenzyme A derivatives,
CC bilirubin, and some other small molecules in the cytoplasm. May be
CC involved in intracellular lipid transport. Binds 2 molecules of cholate
CC per subunit.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- DOMAIN: Forms a beta-barrel structure that accommodates hydrophobic
CC ligands in its interior.
CC -!- SIMILARITY: Belongs to the calycin superfamily. Fatty-acid binding
CC protein (FABP) family. {ECO:0000305}.
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DR EMBL; AF380998; AAK58094.1; -; mRNA.
DR RefSeq; NP_989965.1; NM_204634.1.
DR PDB; 1MVG; NMR; -; A=2-126.
DR PDB; 1TVQ; X-ray; 2.00 A; A=2-126.
DR PDB; 1TW4; X-ray; 2.00 A; A/B=2-126.
DR PDB; 1ZRY; NMR; -; A=2-126.
DR PDB; 2JN3; NMR; -; A=2-126.
DR PDB; 2K62; NMR; -; A=2-126.
DR PDB; 2LFO; NMR; -; A=2-126.
DR PDB; 7O0J; X-ray; 1.40 A; A=1-126.
DR PDB; 7O0K; X-ray; 1.83 A; A/B=1-126.
DR PDBsum; 1MVG; -.
DR PDBsum; 1TVQ; -.
DR PDBsum; 1TW4; -.
DR PDBsum; 1ZRY; -.
DR PDBsum; 2JN3; -.
DR PDBsum; 2K62; -.
DR PDBsum; 2LFO; -.
DR PDBsum; 7O0J; -.
DR PDBsum; 7O0K; -.
DR AlphaFoldDB; P80226; -.
DR BMRB; P80226; -.
DR SMR; P80226; -.
DR STRING; 9031.ENSGALP00000006574; -.
DR iPTMnet; P80226; -.
DR PaxDb; P80226; -.
DR GeneID; 395345; -.
DR KEGG; gga:395345; -.
DR CTD; 395345; -.
DR VEuPathDB; HostDB:geneid_395345; -.
DR eggNOG; KOG4015; Eukaryota.
DR HOGENOM; CLU_113772_4_1_1; -.
DR InParanoid; P80226; -.
DR OrthoDB; 1417203at2759; -.
DR PhylomeDB; P80226; -.
DR TreeFam; TF330348; -.
DR EvolutionaryTrace; P80226; -.
DR PRO; PR:P80226; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR Gene3D; 2.40.128.20; -; 1.
DR InterPro; IPR012674; Calycin.
DR InterPro; IPR000463; Fatty_acid-bd.
DR InterPro; IPR031259; ILBP.
DR PANTHER; PTHR11955; PTHR11955; 1.
DR PRINTS; PR00178; FATTYACIDBP.
DR SUPFAM; SSF50814; SSF50814; 1.
DR PROSITE; PS00214; FABP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cytoplasm; Direct protein sequencing;
KW Lipid-binding; Reference proteome; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:7553344"
FT CHAIN 2..126
FT /note="Fatty acid-binding protein, liver"
FT /id="PRO_0000067338"
FT BINDING 56
FT /ligand="cholate"
FT /ligand_id="ChEBI:CHEBI:29747"
FT /ligand_label="1"
FT /evidence="ECO:0000269|PubMed:15518556"
FT BINDING 57
FT /ligand="cholate"
FT /ligand_id="ChEBI:CHEBI:29747"
FT /ligand_label="1"
FT /evidence="ECO:0000269|PubMed:15518556"
FT BINDING 77
FT /ligand="cholate"
FT /ligand_id="ChEBI:CHEBI:29747"
FT /ligand_label="2"
FT /evidence="ECO:0000269|PubMed:15518556"
FT BINDING 99
FT /ligand="cholate"
FT /ligand_id="ChEBI:CHEBI:29747"
FT /ligand_label="2"
FT /evidence="ECO:0000269|PubMed:15518556"
FT BINDING 101
FT /ligand="cholate"
FT /ligand_id="ChEBI:CHEBI:29747"
FT /ligand_label="2"
FT /evidence="ECO:0000269|PubMed:15518556"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|PubMed:7553344"
FT CONFLICT 92
FT /note="T -> C (in Ref. 1; AAK58094)"
FT /evidence="ECO:0000305"
FT STRAND 5..14
FT /evidence="ECO:0007829|PDB:7O0J"
FT HELIX 15..21
FT /evidence="ECO:0007829|PDB:7O0J"
FT STRAND 22..24
FT /evidence="ECO:0007829|PDB:1MVG"
FT HELIX 26..32
FT /evidence="ECO:0007829|PDB:7O0J"
FT STRAND 38..44
FT /evidence="ECO:0007829|PDB:7O0J"
FT STRAND 47..53
FT /evidence="ECO:0007829|PDB:7O0J"
FT STRAND 58..64
FT /evidence="ECO:0007829|PDB:7O0J"
FT STRAND 69..72
FT /evidence="ECO:0007829|PDB:7O0J"
FT TURN 74..76
FT /evidence="ECO:0007829|PDB:1MVG"
FT STRAND 78..86
FT /evidence="ECO:0007829|PDB:7O0J"
FT STRAND 89..93
FT /evidence="ECO:0007829|PDB:7O0J"
FT STRAND 95..104
FT /evidence="ECO:0007829|PDB:7O0J"
FT STRAND 107..114
FT /evidence="ECO:0007829|PDB:7O0J"
FT STRAND 117..125
FT /evidence="ECO:0007829|PDB:7O0J"
SQ SEQUENCE 126 AA; 14210 MW; 537022389AC431FF CRC64;
MAFSGTWQVY AQENYEEFLK ALALPEDLIK MARDIKPIVE IQQKGDDFVV TSKTPRQTVT
NSFTLGKEAD ITTMDGKKLK CTVHLANGKL VTKSEKFSHE QEVKGNEMVE TITFGGVTLI
RRSKRV
