AI5_USTMA
ID AI5_USTMA Reviewed; 536 AA.
AC Q0H8Y1;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Probable intron-encoded endonuclease aI5;
DE Contains:
DE RecName: Full=Truncated non-functional cytochrome oxidase 1;
DE Contains:
DE RecName: Full=Intron-encoded endonuclease aI5;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN Name=aI5;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RA Kennell J.C., Boehmer C.;
RT "Annotation of mitochondrial genome of Ustilago maydis and comparative
RT analysis of basidiomycete mtDNAs.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
CC -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion. Membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- PTM: The mature protein may arise from proteolytic cleavage of an in-
CC frame translation of COX1 exons 1 to 5 plus intron 5, containing the
CC aI5 open reading frame.
CC -!- SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG
CC endonuclease family. {ECO:0000305}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the heme-copper
CC respiratory oxidase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAZ67025.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ157700; AAZ67025.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AACP01000277; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; YP_762691.1; NC_008368.1.
DR AlphaFoldDB; Q0H8Y1; -.
DR SMR; Q0H8Y1; -.
DR GeneID; 4308276; -.
DR InParanoid; Q0H8Y1; -.
DR Proteomes; UP000000561; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; IBA:GO_Central.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IBA:GO_Central.
DR GO; GO:0022904; P:respiratory electron transport chain; IBA:GO_Central.
DR Gene3D; 1.20.210.10; -; 1.
DR Gene3D; 3.10.28.10; -; 2.
DR InterPro; IPR023616; Cyt_c_oxase-like_su1_dom.
DR InterPro; IPR036927; Cyt_c_oxase-like_su1_sf.
DR InterPro; IPR000883; Cyt_C_Oxase_1.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR004860; LAGLIDADG_2.
DR PANTHER; PTHR10422; PTHR10422; 1.
DR Pfam; PF00115; COX1; 1.
DR Pfam; PF00961; LAGLIDADG_1; 2.
DR PRINTS; PR01165; CYCOXIDASEI.
DR SUPFAM; SSF55608; SSF55608; 2.
DR SUPFAM; SSF81442; SSF81442; 1.
DR PROSITE; PS50855; COX1; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Intron homing; Membrane; Mitochondrion; Nuclease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..?
FT /note="Truncated non-functional cytochrome oxidase 1"
FT /id="PRO_0000271158"
FT CHAIN ?..536
FT /note="Intron-encoded endonuclease aI5"
FT /id="PRO_0000271159"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..235
FT /note="COX1 exons 1 to 5 encoded"
FT REGION 237..536
FT /note="COX1 intron 5 encoded"
SQ SEQUENCE 536 AA; 60704 MW; A6948B6FB2E17324 CRC64;
MVRWLYSTNA KDIGTLYLIF AVFAAMIGTA FSVLIRMELA APGVQYLNGD HQLYNVIITA
HAFVMIFFMV MPAMVGGFGN YLVPVMIGAP DMAFPRLNNI SFWLLPPSLI LLLASAFVEQ
GAGTGWTVYP PLSGLQSHSG GSVDLAIFSL HLSGISSMLG AMNFITTVLN MRNPGMTLHK
LPLFVWAIFV TAILLLLSLP VLAGAITMLL TDRNFNTSFY DPAGGGDPIL YQHLFSKTTL
YISFFYLIYK FTLLKYSIYT NTGTTTVENV FNFNNFYLEY NKTYPNTKVP STSFLEWFVG
FTEGDGSFIV STRGNLMFVI TQSTMDIQVL HYIEQELGFG RVIKQGHKTS RFIVQDMNNL
YILIQLFNGN IVFPSKQNSF FKFVHHFNKL SNFPTVAIIS SLTVPTYYDN WFCGFTDARH
PRGCFTCSLL GNSTAYRFRF LLTQKGEMNK EVLISIANLM KGTVRSHSVK DVYEITVNGI
RNMEKIIDYF TNHKLYSKKA KSYQIWLEIY ESIKNGEHLS PDSRNYLKMK TQQINK
