AI8_USTMA
ID AI8_USTMA Reviewed; 645 AA.
AC Q0H8Y3;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Probable intron-encoded endonuclease aI8;
DE Contains:
DE RecName: Full=Truncated non-functional cytochrome oxidase 1;
DE Contains:
DE RecName: Full=Intron-encoded endonuclease aI8;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN Name=aI8;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RA Kennell J.C., Boehmer C.;
RT "Annotation of mitochondrial genome of Ustilago maydis and comparative
RT analysis of basidiomycete mtDNAs.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
CC -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion. Membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- PTM: The mature protein may arise from proteolytic cleavage of an in-
CC frame translation of COX1 exons 1 to 8 plus intron 8, containing the
CC aI8 open reading frame.
CC -!- SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG
CC endonuclease family. {ECO:0000305}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the heme-copper
CC respiratory oxidase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAZ67023.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ157700; AAZ67023.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AACP01000277; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; YP_762689.1; NC_008368.1.
DR AlphaFoldDB; Q0H8Y3; -.
DR SMR; Q0H8Y3; -.
DR STRING; 237631.Q0H8Y3; -.
DR GeneID; 4308276; -.
DR InParanoid; Q0H8Y3; -.
DR Proteomes; UP000000561; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; IBA:GO_Central.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IBA:GO_Central.
DR GO; GO:0022904; P:respiratory electron transport chain; IBA:GO_Central.
DR CDD; cd01663; Cyt_c_Oxidase_I; 1.
DR Gene3D; 1.20.210.10; -; 1.
DR Gene3D; 3.10.28.10; -; 2.
DR InterPro; IPR023616; Cyt_c_oxase-like_su1_dom.
DR InterPro; IPR036927; Cyt_c_oxase-like_su1_sf.
DR InterPro; IPR000883; Cyt_C_Oxase_1.
DR InterPro; IPR023615; Cyt_c_Oxase_su1_BS.
DR InterPro; IPR033944; Cyt_c_oxase_su1_dom.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR004860; LAGLIDADG_2.
DR PANTHER; PTHR10422; PTHR10422; 1.
DR Pfam; PF00115; COX1; 1.
DR Pfam; PF00961; LAGLIDADG_1; 1.
DR PRINTS; PR01165; CYCOXIDASEI.
DR SUPFAM; SSF55608; SSF55608; 2.
DR SUPFAM; SSF81442; SSF81442; 1.
DR PROSITE; PS50855; COX1; 1.
DR PROSITE; PS00077; COX1_CUB; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Intron homing; Membrane; Mitochondrion; Nuclease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..?
FT /note="Truncated non-functional cytochrome oxidase 1"
FT /id="PRO_0000271162"
FT CHAIN ?..645
FT /note="Intron-encoded endonuclease aI8"
FT /id="PRO_0000271163"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..368
FT /note="COX1 exons 1 to 8 encoded"
FT REGION 369..645
FT /note="COX1 intron 8 encoded"
SQ SEQUENCE 645 AA; 71743 MW; BBB2F89CE159755A CRC64;
MVRWLYSTNA KDIGTLYLIF AVFAAMIGTA FSVLIRMELA APGVQYLNGD HQLYNVIITA
HAFVMIFFMV MPAMVGGFGN YLVPVMIGAP DMAFPRLNNI SFWLLPPSLI LLLASAFVEQ
GAGTGWTVYP PLSGLQSHSG GSVDLAIFSL HLSGISSMLG AMNFITTVLN MRNPGMTLHK
LPLFVWAIFV TAILLLLSLP VLAGAITMLL TDRNFNTSFY DPAGGGDPIL YQHLFWFFGH
PEVYILIIPG FGMVSHIVSA FSGKPVFGYL GMVYAMFSIG ILGFLVWSHH MYAVGLDVDT
RAYFTAATMI IAVPTGIKIF SWLATLYGGS LRITTPMLFA LGFIALFTIG GLTGVILANA
SLDVALHDLK YKTTTYRNSY YTTGPLYYKP STLSRQQLDA FVVGLIDGDG SLQVNHWRYK
ILQFRLVVKL ADKPINYEML CIIASIYGGN VRRVQKEGGY VIWTVNDKKT FTRTILPLFS
KCAPLTSRMH LQLQFFLVFF NDPNVDLYFK LRDSKYIDRE KVSPLFNKAP SYFSDWLGGF
IESEGSFVNR SSGGSSFSIA QNHDYYLIEA IRDFYGVSHL TISNKFGKVS GYPLYEVSIA
SLAGVTHVIY HCVPLLQGYK YHQLVEFINK SKSLKAVTKE FLNLL
