ID   FABZ1_ENTFA             Reviewed;         144 AA.
AC   Q820V3;
DT   04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ;
DE            EC=4.2.1.59;
DE   AltName: Full=(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase;
DE            Short=(3R)-hydroxymyristoyl-ACP dehydrase;
DE   AltName: Full=Beta-hydroxyacyl-ACP dehydratase;
GN   Name=fabZ1; Synonyms=fabZ-1; OrderedLocusNames=EF_0284;
OS   Enterococcus faecalis (strain ATCC 700802 / V583).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=226185;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700802 / V583;
RX   PubMed=12663927; DOI=10.1126/science.1080613;
RA   Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA   Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA   Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA   DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA   Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA   Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT   "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT   faecalis.";
RL   Science 299:2071-2074(2003).
CC   -!- FUNCTION: Involved in unsaturated fatty acids biosynthesis. Catalyzes
CC       the dehydration of short chain beta-hydroxyacyl-ACPs and long chain
CC       saturated and unsaturated beta-hydroxyacyl-ACPs (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O;
CC         Xref=Rhea:RHEA:13097, Rhea:RHEA-COMP:9925, Rhea:RHEA-COMP:9945,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:78784, ChEBI:CHEBI:78827; EC=4.2.1.59;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the thioester dehydratase family. FabZ
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE016830; AAO80147.1; -; Genomic_DNA.
DR   RefSeq; NP_814076.1; NC_004668.1.
DR   RefSeq; WP_002355197.1; NZ_KE136524.1.
DR   AlphaFoldDB; Q820V3; -.
DR   SMR; Q820V3; -.
DR   STRING; 226185.EF_0284; -.
DR   EnsemblBacteria; AAO80147; AAO80147; EF_0284.
DR   GeneID; 60892793; -.
DR   KEGG; efa:EF0284; -.
DR   PATRIC; fig|226185.45.peg.3045; -.
DR   eggNOG; COG0764; Bacteria.
DR   HOGENOM; CLU_078912_1_1_9; -.
DR   OMA; VMALIPN; -.
DR   BioCyc; MetaCyc:MON-19311; -.
DR   Proteomes; UP000001415; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008659; F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008693; F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047451; F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004317; F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00406; FabZ; 1.
DR   InterPro; IPR013114; FabA_FabZ.
DR   InterPro; IPR010084; FabZ.
DR   InterPro; IPR029069; HotDog_dom_sf.
DR   PANTHER; PTHR30272; PTHR30272; 1.
DR   Pfam; PF07977; FabA; 1.
DR   SUPFAM; SSF54637; SSF54637; 1.
DR   TIGRFAMs; TIGR01750; fabZ; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lipid A biosynthesis; Lipid biosynthesis; Lipid metabolism;
KW   Lyase; Reference proteome.
FT   CHAIN           1..144
FT                   /note="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
FT                   FabZ"
FT                   /id="PRO_0000091676"
FT   ACT_SITE        51
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   144 AA;  16242 MW;  38C7B96CF24840D7 CRC64;
     MKKVMTATEI MEMIPNRYPI CYIDYVDEII PNEKIIATKN VTINEEFFQG HFPGNPTMPG
     VLIIEALAQV GSILILKMDQ FEGETAYIGG INKAKFRQKV VPGDVLKLHF EIVKLRDFVG
     IGKATAYVED KKVCECELTF IVGR