FABZ_FRATT
ID FABZ_FRATT Reviewed; 163 AA.
AC Q5NEQ0;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ {ECO:0000255|HAMAP-Rule:MF_00406};
DE EC=4.2.1.59 {ECO:0000255|HAMAP-Rule:MF_00406};
DE AltName: Full=(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase {ECO:0000255|HAMAP-Rule:MF_00406};
DE Short=(3R)-hydroxymyristoyl-ACP dehydrase {ECO:0000255|HAMAP-Rule:MF_00406};
DE AltName: Full=Beta-hydroxyacyl-ACP dehydratase {ECO:0000255|HAMAP-Rule:MF_00406};
GN Name=fabZ {ECO:0000255|HAMAP-Rule:MF_00406}; OrderedLocusNames=FTT_1570c;
OS Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC Francisellaceae; Francisella.
OX NCBI_TaxID=177416;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCHU S4 / Schu 4;
RX PubMed=15640799; DOI=10.1038/ng1499;
RA Larsson P., Oyston P.C.F., Chain P., Chu M.C., Duffield M., Fuxelius H.-H.,
RA Garcia E., Haelltorp G., Johansson D., Isherwood K.E., Karp P.D.,
RA Larsson E., Liu Y., Michell S., Prior J., Prior R., Malfatti S.,
RA Sjoestedt A., Svensson K., Thompson N., Vergez L., Wagg J.K., Wren B.W.,
RA Lindler L.E., Andersson S.G.E., Forsman M., Titball R.W.;
RT "The complete genome sequence of Francisella tularensis, the causative
RT agent of tularemia.";
RL Nat. Genet. 37:153-159(2005).
CC -!- FUNCTION: Involved in unsaturated fatty acids biosynthesis. Catalyzes
CC the dehydration of short chain beta-hydroxyacyl-ACPs and long chain
CC saturated and unsaturated beta-hydroxyacyl-ACPs. {ECO:0000255|HAMAP-
CC Rule:MF_00406}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O;
CC Xref=Rhea:RHEA:13097, Rhea:RHEA-COMP:9925, Rhea:RHEA-COMP:9945,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78784, ChEBI:CHEBI:78827; EC=4.2.1.59;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00406};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00406}.
CC -!- SIMILARITY: Belongs to the thioester dehydratase family. FabZ
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00406}.
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DR EMBL; AJ749949; CAG46203.1; -; Genomic_DNA.
DR RefSeq; WP_003014877.1; NZ_CP010290.1.
DR RefSeq; YP_170492.1; NC_006570.2.
DR PDB; 5BUY; X-ray; 2.55 A; A/B/C/D/E/F=1-163.
DR PDBsum; 5BUY; -.
DR AlphaFoldDB; Q5NEQ0; -.
DR SMR; Q5NEQ0; -.
DR STRING; 177416.FTT_1570c; -.
DR DNASU; 3191868; -.
DR EnsemblBacteria; CAG46203; CAG46203; FTT_1570c.
DR GeneID; 60806676; -.
DR KEGG; ftu:FTT_1570c; -.
DR eggNOG; COG0764; Bacteria.
DR OMA; FPGRPLM; -.
DR Proteomes; UP000001174; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008659; F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0008693; F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0047451; F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0004317; F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00406; FabZ; 1.
DR InterPro; IPR013114; FabA_FabZ.
DR InterPro; IPR010084; FabZ.
DR InterPro; IPR029069; HotDog_dom_sf.
DR PANTHER; PTHR30272; PTHR30272; 1.
DR Pfam; PF07977; FabA; 1.
DR SUPFAM; SSF54637; SSF54637; 1.
DR TIGRFAMs; TIGR01750; fabZ; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Lipid A biosynthesis; Lipid biosynthesis;
KW Lipid metabolism; Lyase; Reference proteome.
FT CHAIN 1..163
FT /note="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
FT FabZ"
FT /id="PRO_0000091679"
FT ACT_SITE 58
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00406"
FT HELIX 13..19
FT /evidence="ECO:0007829|PDB:5BUY"
FT STRAND 31..36
FT /evidence="ECO:0007829|PDB:5BUY"
FT TURN 37..40
FT /evidence="ECO:0007829|PDB:5BUY"
FT STRAND 41..47
FT /evidence="ECO:0007829|PDB:5BUY"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:5BUY"
FT HELIX 53..56
FT /evidence="ECO:0007829|PDB:5BUY"
FT HELIX 67..84
FT /evidence="ECO:0007829|PDB:5BUY"
FT HELIX 86..89
FT /evidence="ECO:0007829|PDB:5BUY"
FT TURN 90..92
FT /evidence="ECO:0007829|PDB:5BUY"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:5BUY"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:5BUY"
FT STRAND 123..134
FT /evidence="ECO:0007829|PDB:5BUY"
FT STRAND 137..146
FT /evidence="ECO:0007829|PDB:5BUY"
FT STRAND 149..159
FT /evidence="ECO:0007829|PDB:5BUY"
SQ SEQUENCE 163 AA; 18150 MW; CBA05CBAD06CB663 CRC64;
MSQFNQNNKQ IDVMGIRKIL PHRYPFALLD KIVDWSVEDR TIVAQKNVTI NEDFFNGHFP
DFPVMPGVLI VEAMAQATAI LGELMAETLF AHVVEKAGGG RRTFMLAGID KVRVKRPVVP
GDVLVIESRM VKQKNIICTA ESVAKVDGQI VCSAELMAAY KDY
