ID   FABZ_YERPA              Reviewed;         176 AA.
AC   Q1CAM3;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ {ECO:0000255|HAMAP-Rule:MF_00406};
DE            EC=4.2.1.59 {ECO:0000255|HAMAP-Rule:MF_00406};
DE   AltName: Full=(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase {ECO:0000255|HAMAP-Rule:MF_00406};
DE            Short=(3R)-hydroxymyristoyl-ACP dehydrase {ECO:0000255|HAMAP-Rule:MF_00406};
DE   AltName: Full=Beta-hydroxyacyl-ACP dehydratase {ECO:0000255|HAMAP-Rule:MF_00406};
GN   Name=fabZ {ECO:0000255|HAMAP-Rule:MF_00406}; OrderedLocusNames=YPA_0531;
OS   Yersinia pestis bv. Antiqua (strain Antiqua).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=360102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Antiqua;
RX   PubMed=16740952; DOI=10.1128/jb.00124-06;
RA   Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M.,
RA   Worsham P., Chu M.C., Andersen G.L.;
RT   "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516:
RT   evidence of gene reduction in an emerging pathogen.";
RL   J. Bacteriol. 188:4453-4463(2006).
CC   -!- FUNCTION: Involved in unsaturated fatty acids biosynthesis. Catalyzes
CC       the dehydration of short chain beta-hydroxyacyl-ACPs and long chain
CC       saturated and unsaturated beta-hydroxyacyl-ACPs. {ECO:0000255|HAMAP-
CC       Rule:MF_00406}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O;
CC         Xref=Rhea:RHEA:13097, Rhea:RHEA-COMP:9925, Rhea:RHEA-COMP:9945,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:78784, ChEBI:CHEBI:78827; EC=4.2.1.59;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00406};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00406}.
CC   -!- SIMILARITY: Belongs to the thioester dehydratase family. FabZ
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00406}.
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DR   EMBL; CP000308; ABG12499.1; -; Genomic_DNA.
DR   RefSeq; WP_002217656.1; NZ_CP009906.1.
DR   PDB; 5BUX; X-ray; 1.90 A; A/B=1-176.
DR   PDBsum; 5BUX; -.
DR   AlphaFoldDB; Q1CAM3; -.
DR   SMR; Q1CAM3; -.
DR   EnsemblBacteria; ABG12499; ABG12499; YPA_0531.
DR   GeneID; 66844579; -.
DR   KEGG; ypa:YPA_0531; -.
DR   OMA; FPGRPLM; -.
DR   Proteomes; UP000001971; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008659; F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008693; F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047451; F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004317; F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00406; FabZ; 1.
DR   InterPro; IPR013114; FabA_FabZ.
DR   InterPro; IPR010084; FabZ.
DR   InterPro; IPR029069; HotDog_dom_sf.
DR   PANTHER; PTHR30272; PTHR30272; 1.
DR   Pfam; PF07977; FabA; 1.
DR   SUPFAM; SSF54637; SSF54637; 1.
DR   TIGRFAMs; TIGR01750; fabZ; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Lipid A biosynthesis; Lipid biosynthesis;
KW   Lipid metabolism; Lyase.
FT   CHAIN           1..176
FT                   /note="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
FT                   FabZ"
FT                   /id="PRO_0000301938"
FT   ACT_SITE        54
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00406"
FT   HELIX           10..16
FT                   /evidence="ECO:0007829|PDB:5BUX"
FT   STRAND          28..33
FT                   /evidence="ECO:0007829|PDB:5BUX"
FT   TURN            34..36
FT                   /evidence="ECO:0007829|PDB:5BUX"
FT   STRAND          37..43
FT                   /evidence="ECO:0007829|PDB:5BUX"
FT   HELIX           49..53
FT                   /evidence="ECO:0007829|PDB:5BUX"
FT   HELIX           63..82
FT                   /evidence="ECO:0007829|PDB:5BUX"
FT   STRAND          92..101
FT                   /evidence="ECO:0007829|PDB:5BUX"
FT   STRAND          110..121
FT                   /evidence="ECO:0007829|PDB:5BUX"
FT   STRAND          124..133
FT                   /evidence="ECO:0007829|PDB:5BUX"
FT   STRAND          136..146
FT                   /evidence="ECO:0007829|PDB:5BUX"
SQ   SEQUENCE   176 AA;  19631 MW;  3A1602C0A93DD2E2 CRC64;
     MTTDTHTLHI EEILDLLPHR FPFLLVDRVL DFEEGKFLRA VKNVSFNEPF FQGHFPGKPI
     FPGVLILEAM AQATGILAFK SRGKLEPGEL YYFAGIDEAR FKRPVVPGDQ MIMEVEFVKE
     RRGLTRFTGV AKVDGEIVCT ATMMCARSKP AAPAESVVVK PDVVKPDVVN PVVKES