FABZ_YERPE
ID FABZ_YERPE Reviewed; 181 AA.
AC Q8ZH57; Q0WHZ0;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ {ECO:0000255|HAMAP-Rule:MF_00406};
DE EC=4.2.1.59 {ECO:0000255|HAMAP-Rule:MF_00406};
DE AltName: Full=(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase {ECO:0000255|HAMAP-Rule:MF_00406};
DE Short=(3R)-hydroxymyristoyl-ACP dehydrase {ECO:0000255|HAMAP-Rule:MF_00406};
DE AltName: Full=Beta-hydroxyacyl-ACP dehydratase {ECO:0000255|HAMAP-Rule:MF_00406};
GN Name=fabZ {ECO:0000255|HAMAP-Rule:MF_00406};
GN OrderedLocusNames=YPO1055, y3124, YP_2795;
OS Yersinia pestis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis;
RX PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: Involved in unsaturated fatty acids biosynthesis. Catalyzes
CC the dehydration of short chain beta-hydroxyacyl-ACPs and long chain
CC saturated and unsaturated beta-hydroxyacyl-ACPs. {ECO:0000255|HAMAP-
CC Rule:MF_00406}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O;
CC Xref=Rhea:RHEA:13097, Rhea:RHEA-COMP:9925, Rhea:RHEA-COMP:9945,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78784, ChEBI:CHEBI:78827; EC=4.2.1.59;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00406};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00406}.
CC -!- SIMILARITY: Belongs to the thioester dehydratase family. FabZ
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00406}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL590842; CAL19720.1; -; Genomic_DNA.
DR EMBL; AE009952; AAM86674.1; -; Genomic_DNA.
DR EMBL; AE017042; AAS62979.1; -; Genomic_DNA.
DR PIR; AF0129; AF0129.
DR RefSeq; WP_002212142.1; NZ_UHIZ01000001.1.
DR RefSeq; YP_002346098.1; NC_003143.1.
DR PDB; 5BUW; X-ray; 1.80 A; A/B=1-181.
DR PDBsum; 5BUW; -.
DR AlphaFoldDB; Q8ZH57; -.
DR SMR; Q8ZH57; -.
DR STRING; 214092.YPO1055; -.
DR PaxDb; Q8ZH57; -.
DR PRIDE; Q8ZH57; -.
DR DNASU; 1148071; -.
DR EnsemblBacteria; AAM86674; AAM86674; y3124.
DR EnsemblBacteria; AAS62979; AAS62979; YP_2795.
DR GeneID; 57977506; -.
DR KEGG; ype:YPO1055; -.
DR KEGG; ypk:y3124; -.
DR KEGG; ypl:CH46_4089; -.
DR KEGG; ypm:YP_2795; -.
DR KEGG; ypv:BZ15_2515; -.
DR KEGG; ypw:CH59_804; -.
DR PATRIC; fig|214092.21.peg.1343; -.
DR eggNOG; COG0764; Bacteria.
DR HOGENOM; CLU_078912_1_0_6; -.
DR OMA; FPGRPLM; -.
DR Proteomes; UP000000815; Chromosome.
DR Proteomes; UP000001019; Chromosome.
DR Proteomes; UP000002490; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008659; F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0008693; F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0047451; F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0004317; F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00406; FabZ; 1.
DR InterPro; IPR013114; FabA_FabZ.
DR InterPro; IPR010084; FabZ.
DR InterPro; IPR029069; HotDog_dom_sf.
DR PANTHER; PTHR30272; PTHR30272; 1.
DR Pfam; PF07977; FabA; 1.
DR SUPFAM; SSF54637; SSF54637; 1.
DR TIGRFAMs; TIGR01750; fabZ; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Lipid A biosynthesis; Lipid biosynthesis;
KW Lipid metabolism; Lyase; Reference proteome.
FT CHAIN 1..181
FT /note="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
FT FabZ"
FT /id="PRO_0000091768"
FT ACT_SITE 54
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00406"
FT VARIANT 170..174
FT /note="Missing (in strain: KIM5 and 91001)"
FT HELIX 10..16
FT /evidence="ECO:0007829|PDB:5BUW"
FT STRAND 28..33
FT /evidence="ECO:0007829|PDB:5BUW"
FT TURN 34..36
FT /evidence="ECO:0007829|PDB:5BUW"
FT STRAND 37..43
FT /evidence="ECO:0007829|PDB:5BUW"
FT HELIX 49..53
FT /evidence="ECO:0007829|PDB:5BUW"
FT HELIX 63..82
FT /evidence="ECO:0007829|PDB:5BUW"
FT STRAND 90..101
FT /evidence="ECO:0007829|PDB:5BUW"
FT STRAND 110..120
FT /evidence="ECO:0007829|PDB:5BUW"
FT STRAND 125..133
FT /evidence="ECO:0007829|PDB:5BUW"
FT STRAND 136..148
FT /evidence="ECO:0007829|PDB:5BUW"
SQ SEQUENCE 181 AA; 20170 MW; 584FDE7A62F8C943 CRC64;
MTTDTHTLHI EEILDLLPHR FPFLLVDRVL DFEEGKFLRA VKNVSFNEPF FQGHFPGKPI
FPGVLILEAM AQATGILAFK SRGKLEPGEL YYFAGIDEAR FKRPVVPGDQ MIMEVEFVKE
RRGLTRFTGV AKVDGEIVCT ATMMCARSKP AAPAESVVVK PDVVKPDVVK PDVVNPVVKE
S
