FACT_ARATH
ID FACT_ARATH Reviewed; 426 AA.
AC Q9FFQ7; Q8LE88;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Fatty alcohol:caffeoyl-CoA acyltransferase {ECO:0000305};
DE EC=2.3.1.- {ECO:0000305};
DE AltName: Full=Fatty alcohol:caffeoyl-CoA caffeoyl transferase {ECO:0000303|PubMed:22797656};
GN Name=FACT {ECO:0000303|PubMed:22797656};
GN OrderedLocusNames=At5g63560 {ECO:0000312|Araport:AT5G63560};
GN ORFNames=MBK5.2 {ECO:0000312|EMBL:BAB10449.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=22797656; DOI=10.1104/pp.112.201822;
RA Kosma D.K., Molina I., Ohlrogge J.B., Pollard M.;
RT "Identification of an Arabidopsis fatty alcohol:caffeoyl-Coenzyme A
RT acyltransferase required for the synthesis of alkyl hydroxycinnamates in
RT root waxes.";
RL Plant Physiol. 160:237-248(2012).
CC -!- FUNCTION: Involved in the synthesis of alkyl hydroxycinnamates in root
CC waxes. Functions as a fatty alcohol:hydroxy cinnamoyl-CoA
CC acyltransferase with apparent preference for caffeoyl-CoA.
CC {ECO:0000269|PubMed:22797656}.
CC -!- TISSUE SPECIFICITY: Expressed in the outermost circumference of mature
CC roots, the endodermis of young roots and in the seed coat of developing
CC seeds. Expressed in outer integument layer 1 of the seed coat.
CC {ECO:0000269|PubMed:22797656}.
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR EMBL; AB005234; BAB10449.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97769.1; -; Genomic_DNA.
DR EMBL; AY035105; AAK59610.1; -; mRNA.
DR EMBL; AY142537; AAN13119.1; -; mRNA.
DR EMBL; AY085563; AAM62785.1; -; mRNA.
DR RefSeq; NP_201161.1; NM_125751.3.
DR AlphaFoldDB; Q9FFQ7; -.
DR SMR; Q9FFQ7; -.
DR STRING; 3702.AT5G63560.1; -.
DR iPTMnet; Q9FFQ7; -.
DR PaxDb; Q9FFQ7; -.
DR PRIDE; Q9FFQ7; -.
DR ProteomicsDB; 222443; -.
DR EnsemblPlants; AT5G63560.1; AT5G63560.1; AT5G63560.
DR GeneID; 836475; -.
DR Gramene; AT5G63560.1; AT5G63560.1; AT5G63560.
DR KEGG; ath:AT5G63560; -.
DR Araport; AT5G63560; -.
DR TAIR; locus:2160549; AT5G63560.
DR eggNOG; ENOG502QS3E; Eukaryota.
DR HOGENOM; CLU_014546_2_0_1; -.
DR InParanoid; Q9FFQ7; -.
DR OMA; FHTIDFG; -.
DR OrthoDB; 1130893at2759; -.
DR PhylomeDB; Q9FFQ7; -.
DR BioCyc; ARA:AT5G63560-MON; -.
DR PRO; PR:Q9FFQ7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FFQ7; baseline and differential.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR GO; GO:0090430; F:caffeoyl-CoA: alcohol caffeoyl transferase activity; IDA:TAIR.
DR GO; GO:0090431; P:alkyl caffeate ester biosynthetic process; IMP:TAIR.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR Gene3D; 3.30.559.10; -; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
PE 2: Evidence at transcript level;
KW Acyltransferase; Cell wall biogenesis/degradation; Reference proteome;
KW Transferase.
FT CHAIN 1..426
FT /note="Fatty alcohol:caffeoyl-CoA acyltransferase"
FT /id="PRO_0000440781"
FT ACT_SITE 162
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT ACT_SITE 374
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT CONFLICT 404
FT /note="K -> N (in Ref. 4; AAM62785)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 426 AA; 47113 MW; 74F6B81BBE70A574 CRC64;
MADSFELIVT RKEPVLVSPA SETPKGLHYL SNLDQNIAII VKTFYYFKSN SRSNEESYEV
IKKSLSEVLV HYYPAAGRLT ISPEGKIAVD CTGEGVVVVE AEANCGIEKI KKAISEIDQP
ETLEKLVYDV PGARNILEIP PVVVQVTNFK CGGFVLGLGM NHNMFDGIAA MEFLNSWAET
ARGLPLSVPP FLDRTLLRPR TPPKIEFPHN EFEDLEDISG TGKLYSDEKL VYKSFLFGPE
KLERLKIMAE TRSTTFQTLT GFLWRARCQA LGLKPDQRIK LLFAADGRSR FVPELPKGYS
GNGIVFTYCV TTAGEVTLNP LSHSVCLVKR AVEMVNDGFM RSAIDYFEVT RARPSLTATL
LITSWAKLSF HTKDFGWGEP VVSGPVGLPE KEVILFLPCG SDTKSINVLL GLPGSAMKVF
QGIMDI
