ID   FAD12_TRIKI             Reviewed;         369 AA.
AC   Q84UB9;
DT   11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Delta(12)-oleate desaturase {ECO:0000303|PubMed:12464604};
DE            Short=PgFAD2 {ECO:0000303|PubMed:12464604};
DE            EC=1.14.19.- {ECO:0000305};
GN   Name=FAD2 {ECO:0000303|PubMed:12464604};
OS   Trichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Trichosanthes.
OX   NCBI_TaxID=3677 {ECO:0000312|EMBL:AAO37752.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   PubMed=12464604; DOI=10.1074/jbc.m210748200;
RA   Iwabuchi M., Kohno-Murase J., Imamura J.;
RT   "Delta 12-oleate desaturase-related enzymes associated with formation of
RT   conjugated trans-delta 11, cis-delta 13 double bonds.";
RL   J. Biol. Chem. 278:4603-4610(2003).
CC   -!- FUNCTION: Delta(12)-fatty acid desaturase producing in a heterologous
CC       system linoleic acid (18:2(9Z,12Z)) and to a lower extent
CC       hexadecadienoic acid (16:2(9Z,12Z)). {ECO:0000269|PubMed:12464604}.
CC   -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC       involved in metal ion binding. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC       {ECO:0000305}.
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DR   EMBL; AY178445; AAO37752.1; -; mRNA.
DR   AlphaFoldDB; Q84UB9; -.
DR   UniPathway; UPA00658; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016717; F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; IEA:InterPro.
DR   GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR005804; FA_desaturase_dom.
DR   InterPro; IPR021863; FAS_N.
DR   Pfam; PF11960; DUF3474; 1.
DR   Pfam; PF00487; FA_desaturase; 1.
PE   2: Evidence at transcript level;
KW   Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis;
KW   Lipid metabolism; Membrane; Oxidoreductase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..369
FT                   /note="Delta(12)-oleate desaturase"
FT                   /id="PRO_0000434638"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        234..254
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           90..94
FT                   /note="Histidine box-1"
FT                   /evidence="ECO:0000305"
FT   MOTIF           126..130
FT                   /note="Histidine box-2"
FT                   /evidence="ECO:0000305"
FT   MOTIF           300..304
FT                   /note="Histidine box-3"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   369 AA;  43103 MW;  A44777382814EE9B CRC64;
     MEKGVQERVP HAKPPFTLSQ IKKVVPPHCF HRSLLRSFSY LLSDLAFVSL FFYLAIAYFP
     LLPHPFPYIA WPLYWIFQGC SLTGVWVIAH ECGHHAFSDY QLIDDIVGLF FHSALLVPYF
     SWKYSHRRHH SNTGSLERDE VFVPKPKSQI PWYLKYFNNP PGRLISLIGT LTLGWPLYLA
     FNMSGRPYDR FACHYDPYSP IFTKREWIQV YISDAGILGM AFMLYRIGVE KGSFWVMRIY
     GIPLVIVNGF LVLITYLQHT HPALPHYESS EWDWLRGALA TVDRDYGVLN RVFHNITDTH
     VVHHLFSTMP HYHAKEATEA VKPVLGEYYR LDRTPVLKAI WREAKECVYV EPDEDADSDK
     GVVWYRKKL