FAD21_MYCTU
ID FAD21_MYCTU Reviewed; 578 AA.
AC P9WQ49; L0T5X7; O50441; P63523;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 34.
DE RecName: Full=Putative fatty-acid--CoA ligase FadD21;
DE EC=6.2.1.-;
DE AltName: Full=Acyl-CoA synthetase;
GN Name=fadD21; OrderedLocusNames=Rv1185c; ORFNames=MTV005.21c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP PUPYLATION AT LYS-355, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=20066036; DOI=10.1371/journal.pone.0008589;
RA Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E.,
RA Gygi S.P., Darwin K.H.;
RT "Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium
RT tuberculosis.";
RL PLoS ONE 5:E8589-E8589(2010).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [4]
RP DISRUPTION PHENOTYPE.
RC STRAIN=H37Rv;
RX PubMed=25124040; DOI=10.1074/jbc.m114.581199;
RA Belardinelli J.M., Larrouy-Maumus G., Jones V., Sorio de Carvalho L.P.,
RA McNeil M.R., Jackson M.;
RT "Biosynthesis and translocation of unsulfated acyltrehaloses in
RT Mycobacterium tuberculosis.";
RL J. Biol. Chem. 289:27952-27965(2014).
CC -!- DISRUPTION PHENOTYPE: Disruption results in the complete loss of
CC diacyltrehalose (DAT) and polyacyltrehalose (PAT) biosynthesis.
CC {ECO:0000269|PubMed:25124040}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP43941.1; -; Genomic_DNA.
DR PIR; A70877; A70877.
DR RefSeq; NP_215701.1; NC_000962.3.
DR RefSeq; WP_003406196.1; NZ_NVQJ01000025.1.
DR AlphaFoldDB; P9WQ49; -.
DR SMR; P9WQ49; -.
DR STRING; 83332.Rv1185c; -.
DR PaxDb; P9WQ49; -.
DR PRIDE; P9WQ49; -.
DR DNASU; 886065; -.
DR GeneID; 886065; -.
DR KEGG; mtu:Rv1185c; -.
DR TubercuList; Rv1185c; -.
DR eggNOG; COG0318; Bacteria.
DR OMA; QGVIAPI; -.
DR PhylomeDB; P9WQ49; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0070566; F:adenylyltransferase activity; IBA:GO_Central.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IBA:GO_Central.
DR CDD; cd05931; FAAL; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; -; 1.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR040097; FAAL/FAAC.
DR Pfam; PF00501; AMP-binding; 1.
PE 1: Evidence at protein level;
KW Fatty acid metabolism; Isopeptide bond; Ligase; Lipid metabolism;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..578
FT /note="Putative fatty-acid--CoA ligase FadD21"
FT /id="PRO_0000193136"
FT CROSSLNK 355
FT /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT with Q-Cter in protein Pup)"
FT /evidence="ECO:0000269|PubMed:20066036"
SQ SEQUENCE 578 AA; 62757 MW; 698FE8FE9E4BFA2F CRC64;
MSDSSVLSLL RERAGLQPDD AAFTYIDYEQ DWAGITETLT WSEVFRRTRI VAHEVRRHCT
TGDRAVILAP QGLAYIAAFL GSMQAGAIAV PLSVPQIGSH DERVSAVLAD ASPSVILTTS
AVAEAVAEHI HRPNTNNVGP IIEIDSLDLT GNSPSFRVKD LPSAAYLQYT SGSTRAPAGV
MISHRNLQAN FQQLMSNYFG DRNGVAPPDT TIVSWLPFYH DMGLVLGIIA PILGGYRSEL
TSPLAFLQRP ARWLHSLANG SPSWSAAPNF AFELAVRKTT DADIEGLDLG NVLGITSGAE
RVHPNTLSRF CNRFAPYNFR EDMIRPSYGL AEATLYVASR NSGDKPEVVY FEPDKLSTGS
ANRCEPKTGT PLLSYGMPTS PTVRIVDPDT CIECPAGTIG EIWVKGDNVA EGYWNKPDET
RHTFGAMLVH PSAGTPDGSW LRTGDLGFLS EDEMFIVGRM KDMLIVYGRN HYPEDIESTV
QEITGGRVAA ISVPVDHTEK LVTVIELKLL GDSAGEAMDE LDVIKNNVTA AISRSHGLNV
ADLVLVPPGS IPTTTSGKIR RAACVEQYRL QQFTRLDG