ID   FAD25_MYCTU             Reviewed;         583 AA.
AC   P9WQ45; L0T8I2; Q50586;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Putative fatty-acid--CoA ligase fadD25;
DE            EC=6.2.1.-;
DE   AltName: Full=Acyl-CoA synthetase;
GN   Name=fadD25; OrderedLocusNames=Rv1521; ORFNames=MTCY19G5.07;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP44285.1; -; Genomic_DNA.
DR   PIR; A70723; A70723.
DR   RefSeq; NP_216037.1; NC_000962.3.
DR   RefSeq; WP_003901187.1; NZ_NVQJ01000004.1.
DR   AlphaFoldDB; P9WQ45; -.
DR   SMR; P9WQ45; -.
DR   STRING; 83332.Rv1521; -.
DR   PaxDb; P9WQ45; -.
DR   DNASU; 886448; -.
DR   GeneID; 45425502; -.
DR   GeneID; 886448; -.
DR   KEGG; mtu:Rv1521; -.
DR   TubercuList; Rv1521; -.
DR   eggNOG; COG0318; Bacteria.
DR   OMA; QIMTAYY; -.
DR   PhylomeDB; P9WQ45; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0070566; F:adenylyltransferase activity; IBA:GO_Central.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IBA:GO_Central.
DR   CDD; cd05931; FAAL; 1.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   InterPro; IPR040097; FAAL/FAAC.
DR   Pfam; PF00501; AMP-binding; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Fatty acid metabolism; Ligase; Lipid metabolism; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..583
FT                   /note="Putative fatty-acid--CoA ligase fadD25"
FT                   /id="PRO_0000193137"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        229..249
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          353..375
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   583 AA;  63142 MW;  1FC1A9B661FDE427 CRC64;
     MSVVESSLPG VLRERASFQP NDKALTFIDY ERSWDGVEET LTWSQLYRRT LNLAAQLREH
     GSTGDRALIL APQSLDYVVS FIASLQAGIV AVPLSIPQGG AHDERTVSVF ADTAPAIVLT
     ASSVVDNVVE YVQPQPGQNA PAVIEVDRLD LDARPSSGSR SAAHGHPDIL YLQYTSGSTR
     TPAGVMVSNK NLFANFEQIM TSYYGVYGKV APPGSTVVSW LPFYHDMGFV LGLILPILAG
     IPAVLTSPIG FLQRPARWIQ MLASNTLAFT AAPNFAFDLA SRKTKDEDME GLDLGGVHGI
     LNGSERVQPV TLKRFIDRFA PFNLDPKAIR PSYGMAEATV YVATRKAGQP PKIVQFDPQK
     LPDGQAERTE SDGGTPLVSY GIVDTQLVRI VDPDTGIERP AGTIGEIWVH GDNVAIGYWQ
     KPEATERTFS ATIVNPSEGT PAGPWLRTGD SGFLSEGELF IMGRIKDLLI VYGRNHSPDD
     IEATIQTISP GRCAAIAVSE HGAEKLVAII ELKKKDESDD EAAERLGFVK REVTSAISKS
     HGLSVADLVL VSPGSIPITT SGKIRRAQCV ELYRQDEFTR LDA