FAD2_CALOF
ID FAD2_CALOF Reviewed; 383 AA.
AC Q9AT72;
DT 17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Delta(12) fatty acid desaturase FAD2 {ECO:0000303|PubMed:11161042};
DE Short=CoFad2 {ECO:0000303|PubMed:11161042};
DE EC=1.14.19.- {ECO:0000305};
GN Name=FAD2 {ECO:0000303|PubMed:11161042};
OS Calendula officinalis (Pot marigold).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae; Calenduleae;
OC Calendula.
OX NCBI_TaxID=41496 {ECO:0000312|EMBL:AAK26633.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=11161042; DOI=10.1104/pp.125.2.847;
RA Qiu X., Reed D.W., Hong H., MacKenzie S.L., Covello P.S.;
RT "Identification and analysis of a gene from Calendula officinalis encoding
RT a fatty acid conjugase.";
RL Plant Physiol. 125:847-855(2001).
CC -!- FUNCTION: Catalyzes the desaturation of oleic acid to linoleic acid.
CC Introduces a double bond at position 12 of 16:1(9Z) and 18:1(9Z).
CC {ECO:0000269|PubMed:11161042}.
CC -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, flower buds and developing
CC seeds.
CC -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC involved in metal ion binding. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC {ECO:0000305}.
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DR EMBL; AF343065; AAK26633.1; -; mRNA.
DR AlphaFoldDB; Q9AT72; -.
DR UniPathway; UPA00658; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016717; F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; IDA:UniProtKB.
DR GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IDA:UniProtKB.
DR InterPro; IPR005804; FA_desaturase_dom.
DR InterPro; IPR021863; FAS_N.
DR Pfam; PF11960; DUF3474; 1.
DR Pfam; PF00487; FA_desaturase; 1.
PE 2: Evidence at transcript level;
KW Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis;
KW Lipid metabolism; Membrane; Oxidoreductase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..383
FT /note="Delta(12) fatty acid desaturase FAD2"
FT /id="PRO_0000435420"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 106..110
FT /note="Histidine box-1"
FT /evidence="ECO:0000305"
FT MOTIF 142..146
FT /note="Histidine box-2"
FT /evidence="ECO:0000305"
FT MOTIF 316..320
FT /note="Histidine box-3"
FT /evidence="ECO:0000305"
SQ SEQUENCE 383 AA; 43996 MW; 2FCAD57AA56EF667 CRC64;
MGAGGRMQDP TNGGNKTEPE PIQRVPHEKP PFTVGDIKKA IPPHCFNRSV IRSFSYVFYD
LTIASILYYI ANNYISTLPS PLAYVAWPVY WAVQGCVLTG VWVIAHECGH HAFSDHQWLD
DTVGLVLHSF LLVPYFSWKY SHRRHHSNTG SIEHDEVFVP KLKSGVRSTA RYLNNPPGRI
LTLLVTLTLG WPLYLTFNVS GRYYDRFACH FDPNSPIYSK RERAQIFISD AGILAVVFVL
FRLAMTKGLT WVLTMYGGPL LVVNGFLVLI TFLQHTHPSL PHYDSTEWDW LRGALTTIDR
DYGILNKVFH NITDTHVAHH LFSTMPHYHA MEATKVIKPI LGDYYQFDGT SIFKAMYRET
KECIYVDKDE EVKDGVYWYR NKI
