FAD3C_BRANA
ID FAD3C_BRANA Reviewed; 404 AA.
AC P48618;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Omega-3 fatty acid desaturase, chloroplastic;
DE EC=1.14.19.-;
DE Flags: Precursor; Fragment;
GN Name=FAD7;
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Seed;
RX PubMed=8029334; DOI=10.1104/pp.103.2.467;
RA Yadav N.S., Wierzbicki A., Aegerter M., Caster C.S., Perez-Grau L.,
RA Kinney A.J., Hitz W.D., Booth J.R. Jr., Schweiger B., Stecca K.L.,
RA Allen S.M., Blackwell M., Reiter R.S., Carlson T.J., Russell S.H.,
RA Feldmann K.A., Pierce J., Browse J.;
RT "Cloning of higher plant omega-3 fatty acid desaturases.";
RL Plant Physiol. 103:467-476(1993).
CC -!- FUNCTION: Chloroplast omega-3 fatty acid desaturase introduces the
CC third double bond in the biosynthesis of 16:3 and 18:3 fatty acids,
CC important constituents of plant membranes. It is thought to use
CC ferredoxin as an electron donor and to act on fatty acids esterified to
CC galactolipids, sulfolipids and phosphatidylglycerol.
CC -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}.
CC -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC involved in metal ion binding.
CC -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA61774.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L22963; AAA61774.1; ALT_INIT; mRNA.
DR PIR; PQ0812; PQ0812.
DR AlphaFoldDB; P48618; -.
DR UniPathway; UPA00658; -.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016717; F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; IEA:InterPro.
DR GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR005804; FA_desaturase_dom.
DR InterPro; IPR021863; FAS_N.
DR Pfam; PF11960; DUF3474; 1.
DR Pfam; PF00487; FA_desaturase; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase; Plastid;
KW Transit peptide.
FT TRANSIT <1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..404
FT /note="Omega-3 fatty acid desaturase, chloroplastic"
FT /id="PRO_0000007118"
FT REGION 28..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 121..125
FT /note="Histidine box-1"
FT MOTIF 157..161
FT /note="Histidine box-2"
FT MOTIF 324..328
FT /note="Histidine box-3"
FT NON_TER 1
SQ SEQUENCE 404 AA; 46617 MW; 4B58FB2F36E2EDE4 CRC64;
FKFRQSPSSP RFRLNSRNWA LNVTTPLTVD SSSSPPIEEE PKTQRFDPGA PPPFNLADIR
AAIPKHCWVK NPWKSMSYVV RELAIVFALA AGAAYLNNWL VWPLYWIAQG TMFWALFVLG
HDCGHGSFSN DPRLNSVVGH LLHSSILVPY HGWRISHRTH HQNHGHVEND ESWHPMSEKI
YKSLDKPTRF FRFTLPLVML AYPFYLWARS PGKKGSHYHP DSDLFLPKER NDVLTSTACW
TAMAVLLVCL NFVMGPMQML KLYVIPYWIN VMWLDFVTYL HHHGHEDKLP WYRGKEWSYL
RGGLTTLDRD YGLINNIHHD IGTHVIHHLF PQIPHYHLVE ATEAAKPVLG KYYREPDKSG
PLPLHLLGIL AKSIKEDHFV SDEGDVVYYE ADPNLYGEIK VTAE
