ID   FAD3C_RICCO             Reviewed;         460 AA.
AC   P48619;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Omega-3 fatty acid desaturase, chloroplastic;
DE            EC=1.14.19.-;
DE   Flags: Precursor;
GN   Name=FAD7A-1;
OS   Ricinus communis (Castor bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC   Ricinus.
OX   NCBI_TaxID=3988;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Baker 296; TISSUE=Seed;
RX   PubMed=8029360; DOI=10.1104/pp.105.1.443;
RA   van de Loo F.J., Somerville C.R.;
RT   "Plasmid omega-3 fatty acid desaturase cDNA from Ricinus communis.";
RL   Plant Physiol. 105:443-444(1994).
CC   -!- FUNCTION: Chloroplast omega-3 fatty acid desaturase introduces the
CC       third double bond in the biosynthesis of 16:3 and 18:3 fatty acids,
CC       important constituents of plant membranes. It is thought to use
CC       ferredoxin as an electron donor and to act on fatty acids esterified to
CC       galactolipids, sulfolipids and phosphatidylglycerol.
CC   -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC       Peripheral membrane protein {ECO:0000305}.
CC   -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC       involved in metal ion binding.
CC   -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC       {ECO:0000305}.
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DR   EMBL; L25897; AAA73511.1; -; mRNA.
DR   PIR; T10063; T10063.
DR   RefSeq; NP_001310643.1; NM_001323714.1.
DR   AlphaFoldDB; P48619; -.
DR   STRING; 3988.XP_002528299.1; -.
DR   GeneID; 8265360; -.
DR   KEGG; rcu:8265360; -.
DR   eggNOG; ENOG502QQQ2; Eukaryota.
DR   InParanoid; P48619; -.
DR   OrthoDB; 577374at2759; -.
DR   UniPathway; UPA00658; -.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016717; F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; IEA:InterPro.
DR   GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR005804; FA_desaturase_dom.
DR   InterPro; IPR021863; FAS_N.
DR   Pfam; PF11960; DUF3474; 1.
DR   Pfam; PF00487; FA_desaturase; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..460
FT                   /note="Omega-3 fatty acid desaturase, chloroplastic"
FT                   /id="PRO_0000007119"
FT   MOTIF           177..181
FT                   /note="Histidine box-1"
FT   MOTIF           213..217
FT                   /note="Histidine box-2"
FT   MOTIF           380..384
FT                   /note="Histidine box-3"
SQ   SEQUENCE   460 AA;  52561 MW;  836592904EF3C7B0 CRC64;
     MAAGWVLSEC GLRPLPRIYS RPRIGFTSKT TNLLKLRELP DSKSYNLCSS FKVSSWSNSK
     QSNWALNVAV PVNVSTVSGE DDREREEFNG IVNVDEGKGE FFDAGAPPPF TLADIRAAIP
     KHCWVKNPWR SMSYVLRDVV VVFGLAAVAA YFNNWVAWPL YWFCQGTMFW ALFVLGHDCG
     HGSFSNNPKL NSVVGHLLHS SILVPYHGWR ISHRTHHQNH GHVENDESWH PLSEKIFKSL
     DNVTKTLRFS LPFPMLAYPF YLWSRSPGKK GSHFHPDSGL FVPKERKDII TSTACWTAMA
     ALLVYLNFSM GPVQMLKLYG IPYWIFVMWL DFVTYLHHHG HEDKLPWYRG KAWSYLRGGL
     TTLDRDYGWI NNIHHDIGTH VIHHLFPQIP HYHLVEATEA AKPVMGKYYR EPKKSGPLPL
     HLLGSLVRSM KEDHYVSDTG DVVYYQKDPK LSGIGGEKTE