ID   FAD3C_SESIN             Reviewed;         447 AA.
AC   P48620;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Omega-3 fatty acid desaturase, chloroplastic;
DE            EC=1.14.19.-;
DE   Flags: Precursor;
GN   Name=FAD7;
OS   Sesamum indicum (Oriental sesame) (Sesamum orientale).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Pedaliaceae; Sesamum.
OX   NCBI_TaxID=4182;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. 4294; TISSUE=Cotyledon;
RA   Shoji K.;
RT   "Sesamum indicum chloroplast omega-3 fatty acid desaturase mRNA, complete
RT   cds.";
RL   Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Chloroplast omega-3 fatty acid desaturase introduces the
CC       third double bond in the biosynthesis of 16:3 and 18:3 fatty acids,
CC       important constituents of plant membranes. It is thought to use
CC       ferredoxin as an electron donor and to act on fatty acids esterified to
CC       galactolipids, sulfolipids and phosphatidylglycerol.
CC   -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC       Peripheral membrane protein {ECO:0000305}.
CC   -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC       involved in metal ion binding.
CC   -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC       {ECO:0000305}.
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DR   EMBL; U25817; AAA70334.1; -; mRNA.
DR   RefSeq; NP_001306619.1; NM_001319690.1.
DR   RefSeq; XP_011084901.1; XM_011086599.1.
DR   AlphaFoldDB; P48620; -.
DR   GeneID; 105167042; -.
DR   KEGG; sind:105167042; -.
DR   OrthoDB; 577374at2759; -.
DR   BRENDA; 1.14.19.35; 5697.
DR   UniPathway; UPA00658; -.
DR   Proteomes; UP000504604; Linkage group LG7.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016717; F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; IEA:InterPro.
DR   GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR005804; FA_desaturase_dom.
DR   InterPro; IPR021863; FAS_N.
DR   Pfam; PF11960; DUF3474; 1.
DR   Pfam; PF00487; FA_desaturase; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase; Plastid;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..447
FT                   /note="Omega-3 fatty acid desaturase, chloroplastic"
FT                   /id="PRO_0000007120"
FT   MOTIF           167..171
FT                   /note="Histidine box-1"
FT   MOTIF           203..207
FT                   /note="Histidine box-2"
FT   MOTIF           370..374
FT                   /note="Histidine box-3"
SQ   SEQUENCE   447 AA;  51116 MW;  4E76250DDD6DA6B1 CRC64;
     MASWVLSECG LRPLPRVYPK PRTGHPLLNS NPTKLRFSRT DLGNGSSFCL SSGILREKNW
     ALRVSAPLRV LQVEEEEENK EGERVINGGE EFDPGAPPPF KLSDIREAIP KHCWVKDPWR
     SMGYVVRDVA VVFGLAAVAA YFNNWVVWPL YWFAQSTMFW ALFVLGHDCG HGSFSNDPKL
     NSVVGHILHS SILVPYHGWR ISHRTHHQNH GHVENDESWH PLSEKIYKNL DTATKKLRFT
     LPFPLLAYPI YLWSRSPGKQ GSHFHPDSDL FVPNEKKDVI TSTVCWTAML ALLVGLSFVI
     GPVQLLKLYG IPYLGNVMWL DLVTYLHHHG HEDKLPWYRG KEWSYLRGGL TTLDRDYGWI
     NNIHHDIGTH VIHHLFPQIP HYHLIEATEA AKPVLGKYYR EPKKSAPLPF HLLGDLTRSL
     KRDHYVSDVG DVVYYQTDPQ LTGAEKS