ID   FAD3E_SOYBN             Reviewed;         380 AA.
AC   P48625;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Omega-3 fatty acid desaturase, endoplasmic reticulum;
DE            EC=1.14.19.-;
GN   Name=FAD3;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Seed;
RX   PubMed=8029334; DOI=10.1104/pp.103.2.467;
RA   Yadav N.S., Wierzbicki A., Aegerter M., Caster C.S., Perez-Grau L.,
RA   Kinney A.J., Hitz W.D., Booth J.R. Jr., Schweiger B., Stecca K.L.,
RA   Allen S.M., Blackwell M., Reiter R.S., Carlson T.J., Russell S.H.,
RA   Feldmann K.A., Pierce J., Browse J.;
RT   "Cloning of higher plant omega-3 fatty acid desaturases.";
RL   Plant Physiol. 103:467-476(1993).
CC   -!- FUNCTION: Microsomal (ER) omega-3 fatty acid desaturase introduces the
CC       third double bond in the biosynthesis of 18:3 fatty acids, important
CC       constituents of plant membranes. It is thought to use cytochrome b5 as
CC       an electron donor and to act on fatty acids esterified to
CC       phosphatidylcholine and, possibly, other phospholipids.
CC   -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein.
CC   -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC       involved in metal ion binding.
CC   -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC       {ECO:0000305}.
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DR   EMBL; L22964; AAA61777.1; -; mRNA.
DR   PIR; JQ2338; JQ2338.
DR   RefSeq; NP_001237507.1; NM_001250578.1.
DR   AlphaFoldDB; P48625; -.
DR   STRING; 3847.GLYMA02G39230.1; -.
DR   eggNOG; ENOG502QTIC; Eukaryota.
DR   InParanoid; P48625; -.
DR   UniPathway; UPA00658; -.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016717; F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; IEA:InterPro.
DR   GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR005804; FA_desaturase_dom.
DR   InterPro; IPR021863; FAS_N.
DR   Pfam; PF11960; DUF3474; 1.
DR   Pfam; PF00487; FA_desaturase; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..380
FT                   /note="Omega-3 fatty acid desaturase, endoplasmic
FT                   reticulum"
FT                   /id="PRO_0000185414"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        212..232
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        236..256
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           100..104
FT                   /note="Histidine box-1"
FT   MOTIF           136..140
FT                   /note="Histidine box-2"
FT   MOTIF           303..307
FT                   /note="Histidine box-3"
SQ   SEQUENCE   380 AA;  44185 MW;  BF800F93CF4C29D7 CRC64;
     MVKDTKPLAY AANNGYQQKG SSFDFDPSAP PPFKIAEIRA SIPKHCWVKN PWRSLSYVLR
     DVLVIAALVA AAIHFDNWLL WLIYCPIQGT MFWALFVLGH DCGHGSFSDS PLLNSLVGHI
     LHSSILVPYH GWRISHRTHH QNHGHIEKDE SWVPLTEKIY KNLDSMTRLI RFTVPFPLFV
     YPIYLFSRSP GKEGSHFNPY SNLFPPSERK GIAISTLCWA TMFSLLIYLS FITSPLLVLK
     LYGIPYWIFV MWLDFVTYLH HHGHHQKLPW YRGKEWSYLR GGLTTVDRDY GWIYNIHHDI
     GTHVIHHLFP QIPHYHLVEA TQAAKPVLGD YYREPERSAP LPFHLIKYLI QSMRQDHFVS
     DTGDVVYYQT DSLLLHSQRD