ID   FAD3_RICPR              Reviewed;         720 AA.
AC   Q9ZCZ1;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Putative fatty acid oxidation complex trifunctional enzyme;
DE   Includes:
DE     RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
DE              EC=1.1.1.35;
DE   Includes:
DE     RecName: Full=Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase;
DE              EC=4.2.1.17;
DE              EC=5.3.3.8;
GN   OrderedLocusNames=RP560;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC         NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3Z)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA;
CC         Xref=Rhea:RHEA:45900, ChEBI:CHEBI:85097, ChEBI:CHEBI:85489;
CC         EC=5.3.3.8;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA;
CC         Xref=Rhea:RHEA:45228, ChEBI:CHEBI:58521, ChEBI:CHEBI:85097;
CC         EC=5.3.3.8;
CC   -!- SIMILARITY: In the N-terminal section; belongs to the 3-hydroxyacyl-CoA
CC       dehydrogenase family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the enoyl-CoA
CC       hydratase/isomerase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ235272; CAA15008.1; -; Genomic_DNA.
DR   PIR; F71660; F71660.
DR   RefSeq; NP_220931.1; NC_000963.1.
DR   RefSeq; WP_004599039.1; NC_000963.1.
DR   AlphaFoldDB; Q9ZCZ1; -.
DR   SMR; Q9ZCZ1; -.
DR   STRING; 272947.RP560; -.
DR   EnsemblBacteria; CAA15008; CAA15008; CAA15008.
DR   GeneID; 57569682; -.
DR   KEGG; rpr:RP560; -.
DR   PATRIC; fig|272947.5.peg.573; -.
DR   eggNOG; COG1024; Bacteria.
DR   eggNOG; COG1250; Bacteria.
DR   HOGENOM; CLU_010448_0_0_5; -.
DR   OMA; GQGFYKK; -.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004165; F:delta(3)-delta(2)-enoyl-CoA isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR   GO; GO:0044248; P:cellular catabolic process; IEA:UniProt.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR045004; ECH_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   Pfam; PF16113; ECH_2; 1.
DR   SUPFAM; SSF48179; SSF48179; 2.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Isomerase; Lipid metabolism; Lyase;
KW   Multifunctional enzyme; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..720
FT                   /note="Putative fatty acid oxidation complex trifunctional
FT                   enzyme"
FT                   /id="PRO_0000285698"
FT   REGION          1..384
FT                   /note="3-hydroxyacyl-CoA dehydrogenase"
FT   REGION          453..720
FT                   /note="Enoyl-CoA hydratase/isomerase"
SQ   SEQUENCE   720 AA;  81403 MW;  BAEC37A40044234E CRC64;
     MQNEIKKVCV IGAGVMGSGI AALIANSSHQ VVLLDILDKD SNDPNKIVKN SVKNLHKQKL
     SPLSFPDKVN FITIGNLEHD LDLIKECNLV IEVIVEKLEI KHQLYNKIIP YLKEDAIIAS
     NTSTLPLKKL KVNLPNNIKS RFVITHFFNP PRYMELVELI IDHTIKDEVI EKVSVFLTKM
     LGKTIIKCND TPGFIANRVG CFLLELVVHK AIAQNLNFVT IDQIFSRCLG LPNTGIFGLY
     DLIGHDVMKL ISSSLISALP KSDDYHRIYT NTKVFDKMIE HNLIGRKGEG GFYRLSVSNG
     KKIKEVINIS DLSYHPVQKV DISFNSLNEL LSSNSIYGKF FSEIITEFYI YLTSLVPSVT
     NNIYDIDTTM KLGYSWHYGP FELLTIAVKN GWNLIIKNAD LMNIPLPKYL ASKEYQKIDK
     QKFNSKKDFL QESKIVLSND SANLIHYCEN LVFVITTKMN SLNHNVFYLL QEAVSKAENY
     GKNLYIYPQG NNFSAGADLK LILSYIQDGN FHNLENLLKL GQQTMRYLKY SSVHIISCAR
     GVALGGGCEL LLNSSYIVAN QELNAGLIEL GVGLIPGWTG VTEMFARSNG NKTKLIRNIK
     NIIEQNKTSS ADYFKADYGI KNMQVNMNKH YILDDALKLR ISKKIVSIPN KITLPKINIV
     SEIDTSKYNE LQNKVLNKFQ NIIDKHNEIS EAELLTYERE MFLELAKTPQ TIEKLQAIVG