FAD3_RICTY
ID FAD3_RICTY Reviewed; 720 AA.
AC Q68WH7;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Putative fatty acid oxidation complex trifunctional enzyme;
DE Includes:
DE RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
DE EC=1.1.1.35;
DE Includes:
DE RecName: Full=Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase;
DE EC=4.2.1.17;
DE EC=5.3.3.8;
GN OrderedLocusNames=RT0547;
OS Rickettsia typhi (strain ATCC VR-144 / Wilmington).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX NCBI_TaxID=257363;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC VR-144 / Wilmington;
RX PubMed=15317790; DOI=10.1128/jb.186.17.5842-5855.2004;
RA McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E.,
RA McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E.,
RA Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A., Hong C.,
RA Yu X.-J., Walker D.H., Weinstock G.M.;
RT "Complete genome sequence of Rickettsia typhi and comparison with sequences
RT of other Rickettsiae.";
RL J. Bacteriol. 186:5842-5855(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:58856; EC=4.2.1.17;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC ChEBI:CHEBI:137480; EC=4.2.1.17;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3Z)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA;
CC Xref=Rhea:RHEA:45900, ChEBI:CHEBI:85097, ChEBI:CHEBI:85489;
CC EC=5.3.3.8;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA;
CC Xref=Rhea:RHEA:45228, ChEBI:CHEBI:58521, ChEBI:CHEBI:85097;
CC EC=5.3.3.8;
CC -!- SIMILARITY: In the N-terminal section; belongs to the 3-hydroxyacyl-CoA
CC dehydrogenase family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the enoyl-CoA
CC hydratase/isomerase family. {ECO:0000305}.
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DR EMBL; AE017197; AAU04015.1; -; Genomic_DNA.
DR RefSeq; WP_011190996.1; NC_006142.1.
DR AlphaFoldDB; Q68WH7; -.
DR SMR; Q68WH7; -.
DR STRING; 257363.RT0547; -.
DR EnsemblBacteria; AAU04015; AAU04015; RT0547.
DR KEGG; rty:RT0547; -.
DR eggNOG; COG1024; Bacteria.
DR eggNOG; COG1250; Bacteria.
DR HOGENOM; CLU_010448_0_0_5; -.
DR OMA; GQGFYKK; -.
DR OrthoDB; 82358at2; -.
DR Proteomes; UP000000604; Chromosome.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0004165; F:delta(3)-delta(2)-enoyl-CoA isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0044248; P:cellular catabolic process; IEA:UniProt.
DR GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR045004; ECH_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF16113; ECH_2; 1.
DR SUPFAM; SSF48179; SSF48179; 2.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
PE 3: Inferred from homology;
KW Fatty acid metabolism; Isomerase; Lipid metabolism; Lyase;
KW Multifunctional enzyme; NAD; Oxidoreductase.
FT CHAIN 1..720
FT /note="Putative fatty acid oxidation complex trifunctional
FT enzyme"
FT /id="PRO_0000285699"
FT REGION 1..384
FT /note="3-hydroxyacyl-CoA dehydrogenase"
FT REGION 453..720
FT /note="Enoyl-CoA hydratase/isomerase"
SQ SEQUENCE 720 AA; 81410 MW; 969CD34D859084C6 CRC64;
MQNEIKKVCV IGAGVMGSGI AALIANSSHR VVLLDILDKD SNDPNKIVKN AVKNLHRQKL
PPLSYPDKVN FITIGNLEHD LDLIKECNLV IEVIVEKLDI KHQLYNKIIP YLKEDTIIAS
NTSTLPLKKL KENLPNNIKS RFIITHFFNP PRYMELVELI IDNTIKDEVI EKISVFLTKI
LGKTIIKCND TPGFIANRVG CFLLELVAHK AIAQNLDFVT IDQIFSRCLG LPNTGIFGLY
DLIGHDVMKL ISSSLLSALP KSDDYHRIYF NTKVFDKMIE HNLIGRKGEG GFYRLSVSNG
KKIKEVINIS DLSYHPVQKV DISFNNLDEL LASDSIYSKF FSEIITEFYI YLTSLVPSVT
NNIYDIDATM KLGYSWHYGP FELLTIAVKN GWNSIIKNAD LMNISLPKYL ASKEYQKIDK
QKFNSKKDFL QESTIVLAND SANLIHYCEN LIFVITTKMN TLNHNVFYLL QEAVSKAENY
GKNLYIFPQG NNFSAGADLK LILSYIQDGN FDNLENLLKL GQQTMRYLKY SSVHIISCAR
GVALGGGCEL LLNSSYIVAN QELNAGLIEF GVGLIPGWSG VTEMFARSNG DKTKLIRNIK
NIIEQNKTSS ADYFKADYDV SNMQVNMNKH YILDEALKLN VSNKIVSIPH KIALPKINIV
SEIDTSKYND LQNKVLNKFQ NIIDKHNEIS EAELLTYERE IFLELAKEPQ TIEKLHAIVG
