位置:首页 > 蛋白库 > FAD6C_BRANA
FAD6C_BRANA
ID   FAD6C_BRANA             Reviewed;         443 AA.
AC   P48627;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Omega-6 fatty acid desaturase, chloroplastic {ECO:0000303|PubMed:8066133};
DE            EC=1.14.19.23 {ECO:0000269|PubMed:8066133};
DE   Flags: Precursor;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   TISSUE=Seed;
RX   PubMed=8066133; DOI=10.1104/pp.105.2.635;
RA   Hitz W.D., Carlson T.J., Booth J.R. Jr., Kinney A.J., Stecca K.L.,
RA   Yadav N.S.;
RT   "Cloning of a higher-plant plastid omega-6 fatty acid desaturase cDNA and
RT   its expression in a cyanobacterium.";
RL   Plant Physiol. 105:635-641(1994).
CC   -!- FUNCTION: Chloroplast omega-6 fatty acid desaturase introduces the
CC       second double bond in the biosynthesis of 16:3 and 18:3 fatty acids,
CC       important constituents of plant membranes. It is thought to use
CC       ferredoxin as an electron donor and to act on fatty acids esterified to
CC       galactolipids, sulfolipids and phosphatidylglycerol.
CC       {ECO:0000269|PubMed:8066133}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (9Z)-octadecenoyl-containing glycerolipid + 2 H(+) + O2 + 2
CC         reduced [2Fe-2S]-[ferredoxin] = a (9Z,12Z)-octadecadienoyl-containing
CC         glycerolipid + 2 H2O + 2 oxidized [2Fe-2S]-[ferredoxin];
CC         Xref=Rhea:RHEA:46376, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:88240,
CC         ChEBI:CHEBI:88351; EC=1.14.19.23;
CC         Evidence={ECO:0000269|PubMed:8066133};
CC   -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC       Peripheral membrane protein {ECO:0000305}.
CC   -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC       involved in metal ion binding.
CC   -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L29214; AAA50157.1; -; mRNA.
DR   PIR; T08136; T08136.
DR   AlphaFoldDB; P48627; -.
DR   EnsemblPlants; CDX68706; CDX68706; GSBRNA2T00130387001.
DR   Gramene; CDX68706; CDX68706; GSBRNA2T00130387001.
DR   OMA; NSMFICL; -.
DR   UniPathway; UPA00658; -.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0102873; F:1-18:1-2-16:0-digalactosyldiacylglycerol desaturase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102834; F:1-18:1-2-16:0-monogalactosyldiacylglycerol acyl-lipid omega-6 desaturase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102850; F:1-18:1-2-16:0-phosphatidylglycerol omega-6 desaturase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102836; F:1-18:1-2-16:1-monogalactosyldiacylglyceroldesaturase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102838; F:1-18:1-2-16:2-monogalactosyldiacylglycerol desaturase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102841; F:1-18:1-2-16:2-monogalactosyldiacylglycerol synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102655; F:1-18:1-2-trans-16:1-phosphatidylglycerol desaturase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102844; F:1-18:2-2-16:1-monogalactosyldiacylglycerol desaturase activity (SN2-16:2 forming); IEA:UniProtKB-EC.
DR   GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR005804; FA_desaturase_dom.
DR   Pfam; PF00487; FA_desaturase; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT         1..64
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000250|UniProtKB:P46312"
FT   CHAIN           65..443
FT                   /note="Omega-6 fatty acid desaturase, chloroplastic"
FT                   /id="PRO_0000007124"
FT   MOTIF           166..170
FT                   /note="Histidine box-1"
FT                   /evidence="ECO:0000305"
FT   MOTIF           202..206
FT                   /note="Histidine box-2"
FT                   /evidence="ECO:0000305"
FT   MOTIF           362..366
FT                   /note="Histidine box-3"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   443 AA;  50755 MW;  27544B33AF2781D8 CRC64;
     MASRIADSLF AFTGPQQCLP RAPKLASARL SPGVYAVRPI DLLLKGTRRT FLVPAKKRIG
     CIKAVFVPVA PPSADNAEDR EQLAESYGFK QIGQDLPDNV TLKDIMDTLP KEVFEIDDVK
     AWKSVLISVT SYALGLFMIA KAPWYLLPLA WAWTGTAVTG FFVIGHDCAH KSFSKNKLVE
     DIVGTLAFLP LVYPYEPWRF KHDRHHAKTN MLVHDTAWQP VPPEEFDSSP VLRKAIIFGY
     GPIRPWLSIA HWVNWHFNLR KFRPSEVNRV KISLACVFAF MAVGWPLIIY KVGVLGWVKF
     WLMPWLGYHF WMSTFTMVHH TAPHIPFKPA DEWNAAQAQL NGTVHCDYPS WIEILCHDIN
     VHIPHHISPR IPSYNLRAAH QSIQENWGKY TNLATWNWRL MKTIMTVCHV YDKEENYIPF
     DRLAPEESQP ITFLKKAMPD YAA
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024