FAD6C_BRANA
ID FAD6C_BRANA Reviewed; 443 AA.
AC P48627;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Omega-6 fatty acid desaturase, chloroplastic {ECO:0000303|PubMed:8066133};
DE EC=1.14.19.23 {ECO:0000269|PubMed:8066133};
DE Flags: Precursor;
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC TISSUE=Seed;
RX PubMed=8066133; DOI=10.1104/pp.105.2.635;
RA Hitz W.D., Carlson T.J., Booth J.R. Jr., Kinney A.J., Stecca K.L.,
RA Yadav N.S.;
RT "Cloning of a higher-plant plastid omega-6 fatty acid desaturase cDNA and
RT its expression in a cyanobacterium.";
RL Plant Physiol. 105:635-641(1994).
CC -!- FUNCTION: Chloroplast omega-6 fatty acid desaturase introduces the
CC second double bond in the biosynthesis of 16:3 and 18:3 fatty acids,
CC important constituents of plant membranes. It is thought to use
CC ferredoxin as an electron donor and to act on fatty acids esterified to
CC galactolipids, sulfolipids and phosphatidylglycerol.
CC {ECO:0000269|PubMed:8066133}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (9Z)-octadecenoyl-containing glycerolipid + 2 H(+) + O2 + 2
CC reduced [2Fe-2S]-[ferredoxin] = a (9Z,12Z)-octadecadienoyl-containing
CC glycerolipid + 2 H2O + 2 oxidized [2Fe-2S]-[ferredoxin];
CC Xref=Rhea:RHEA:46376, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:88240,
CC ChEBI:CHEBI:88351; EC=1.14.19.23;
CC Evidence={ECO:0000269|PubMed:8066133};
CC -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}.
CC -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC involved in metal ion binding.
CC -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC {ECO:0000305}.
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DR EMBL; L29214; AAA50157.1; -; mRNA.
DR PIR; T08136; T08136.
DR AlphaFoldDB; P48627; -.
DR EnsemblPlants; CDX68706; CDX68706; GSBRNA2T00130387001.
DR Gramene; CDX68706; CDX68706; GSBRNA2T00130387001.
DR OMA; NSMFICL; -.
DR UniPathway; UPA00658; -.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0102873; F:1-18:1-2-16:0-digalactosyldiacylglycerol desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102834; F:1-18:1-2-16:0-monogalactosyldiacylglycerol acyl-lipid omega-6 desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102850; F:1-18:1-2-16:0-phosphatidylglycerol omega-6 desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102836; F:1-18:1-2-16:1-monogalactosyldiacylglyceroldesaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102838; F:1-18:1-2-16:2-monogalactosyldiacylglycerol desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102841; F:1-18:1-2-16:2-monogalactosyldiacylglycerol synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0102655; F:1-18:1-2-trans-16:1-phosphatidylglycerol desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102844; F:1-18:2-2-16:1-monogalactosyldiacylglycerol desaturase activity (SN2-16:2 forming); IEA:UniProtKB-EC.
DR GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR005804; FA_desaturase_dom.
DR Pfam; PF00487; FA_desaturase; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase; Plastid;
KW Transit peptide.
FT TRANSIT 1..64
FT /note="Chloroplast"
FT /evidence="ECO:0000250|UniProtKB:P46312"
FT CHAIN 65..443
FT /note="Omega-6 fatty acid desaturase, chloroplastic"
FT /id="PRO_0000007124"
FT MOTIF 166..170
FT /note="Histidine box-1"
FT /evidence="ECO:0000305"
FT MOTIF 202..206
FT /note="Histidine box-2"
FT /evidence="ECO:0000305"
FT MOTIF 362..366
FT /note="Histidine box-3"
FT /evidence="ECO:0000305"
SQ SEQUENCE 443 AA; 50755 MW; 27544B33AF2781D8 CRC64;
MASRIADSLF AFTGPQQCLP RAPKLASARL SPGVYAVRPI DLLLKGTRRT FLVPAKKRIG
CIKAVFVPVA PPSADNAEDR EQLAESYGFK QIGQDLPDNV TLKDIMDTLP KEVFEIDDVK
AWKSVLISVT SYALGLFMIA KAPWYLLPLA WAWTGTAVTG FFVIGHDCAH KSFSKNKLVE
DIVGTLAFLP LVYPYEPWRF KHDRHHAKTN MLVHDTAWQP VPPEEFDSSP VLRKAIIFGY
GPIRPWLSIA HWVNWHFNLR KFRPSEVNRV KISLACVFAF MAVGWPLIIY KVGVLGWVKF
WLMPWLGYHF WMSTFTMVHH TAPHIPFKPA DEWNAAQAQL NGTVHCDYPS WIEILCHDIN
VHIPHHISPR IPSYNLRAAH QSIQENWGKY TNLATWNWRL MKTIMTVCHV YDKEENYIPF
DRLAPEESQP ITFLKKAMPD YAA