FAD6C_SOYBN
ID FAD6C_SOYBN Reviewed; 424 AA.
AC P48628;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Omega-6 fatty acid desaturase, chloroplastic {ECO:0000303|PubMed:8066133};
DE EC=1.14.19.23 {ECO:0000250|UniProtKB:P48627};
DE Flags: Precursor;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Seed;
RX PubMed=8066133; DOI=10.1104/pp.105.2.635;
RA Hitz W.D., Carlson T.J., Booth J.R. Jr., Kinney A.J., Stecca K.L.,
RA Yadav N.S.;
RT "Cloning of a higher-plant plastid omega-6 fatty acid desaturase cDNA and
RT its expression in a cyanobacterium.";
RL Plant Physiol. 105:635-641(1994).
CC -!- FUNCTION: Chloroplast omega-6 fatty acid desaturase introduces the
CC second double bond in the biosynthesis of 16:3 and 18:3 fatty acids,
CC important constituents of plant membranes. It is thought to use
CC ferredoxin as an electron donor and to act on fatty acids esterified to
CC galactolipids, sulfolipids and phosphatidylglycerol.
CC {ECO:0000250|UniProtKB:P48627}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (9Z)-octadecenoyl-containing glycerolipid + 2 H(+) + O2 + 2
CC reduced [2Fe-2S]-[ferredoxin] = a (9Z,12Z)-octadecadienoyl-containing
CC glycerolipid + 2 H2O + 2 oxidized [2Fe-2S]-[ferredoxin];
CC Xref=Rhea:RHEA:46376, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:88240,
CC ChEBI:CHEBI:88351; EC=1.14.19.23;
CC Evidence={ECO:0000250|UniProtKB:P48627};
CC -!- PATHWAY: Lipid metabolism; polyunsaturated fatty acid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}.
CC -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC involved in metal ion binding. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family.
CC {ECO:0000305}.
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DR EMBL; L29215; AAA50158.1; -; mRNA.
DR PIR; T07742; T07742.
DR RefSeq; NP_001238236.1; NM_001251307.1.
DR AlphaFoldDB; P48628; -.
DR STRING; 3847.GLYMA02G36460.1; -.
DR GeneID; 547807; -.
DR KEGG; gmx:547807; -.
DR eggNOG; ENOG502QQPI; Eukaryota.
DR UniPathway; UPA00658; -.
DR Proteomes; UP000008827; Unplaced.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0102873; F:1-18:1-2-16:0-digalactosyldiacylglycerol desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102834; F:1-18:1-2-16:0-monogalactosyldiacylglycerol acyl-lipid omega-6 desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102850; F:1-18:1-2-16:0-phosphatidylglycerol omega-6 desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102836; F:1-18:1-2-16:1-monogalactosyldiacylglyceroldesaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102838; F:1-18:1-2-16:2-monogalactosyldiacylglycerol desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102841; F:1-18:1-2-16:2-monogalactosyldiacylglycerol synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0102655; F:1-18:1-2-trans-16:1-phosphatidylglycerol desaturase activity; IEA:UniProtKB-EC.
DR GO; GO:0102844; F:1-18:2-2-16:1-monogalactosyldiacylglycerol desaturase activity (SN2-16:2 forming); IEA:UniProtKB-EC.
DR GO; GO:0006636; P:unsaturated fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR005804; FA_desaturase_dom.
DR Pfam; PF00487; FA_desaturase; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..63
FT /note="Chloroplast"
FT /evidence="ECO:0000250|UniProtKB:P46312"
FT CHAIN 64..424
FT /note="Omega-6 fatty acid desaturase, chloroplastic"
FT /id="PRO_0000007125"
FT MOTIF 165..169
FT /note="Histidine box-1"
FT /evidence="ECO:0000305"
FT MOTIF 201..205
FT /note="Histidine box-2"
FT /evidence="ECO:0000305"
FT MOTIF 361..365
FT /note="Histidine box-3"
FT /evidence="ECO:0000305"
FT MOD_RES 64
FT /note="N-acetylvaline"
FT /evidence="ECO:0000250|UniProtKB:P46312"
SQ SEQUENCE 424 AA; 49641 MW; 4F3DF52D4B1A2009 CRC64;
MACTLADSLL LFKGSYQKPV LRRDIAARYS PGIFSLNSNG LIQKRFRRQR NFVTRNKVTV
IHAVAIPVQP APVESAEYRK QLAEDYGFRQ VGEPLSDDVT LKDVINPLPK EVFEIDDVKA
WKSVLISVTS YALGLFMISK APWYLLPLAW VWTGTAITGF FVIGHDCAHR SFSSNKLVED
IVGTLAFMPL IYPYEPWRFK HDRHHAKTNM LREDTAWHPV WKDEFESTPL LRKAIIYGYG
PFRCWMSIAH WLMWHFDLKK FRPSEVPRVK ISLACVFAFI AIGWPLIIYK TGIMGWIKFW
LMPWLGYHFW MSTFTMVHHT APYIPFKYSE EWNRAQAQLN GTVHCDYPKW IEILCHDINV
HIPHHISPRI PSYNLRAAHK SLQENWGQYL NEASWNWRLM KTIMTVCQVY DKEKSLCCLR
RTCP
