ID   FADB2_MYCTO             Reviewed;         286 AA.
AC   P9WNP6; L0T3U3; O53753; Q7D9R6;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=3-hydroxybutyryl-CoA dehydrogenase;
DE            EC=1.1.1.157 {ECO:0000250|UniProtKB:P9WNP7};
DE   AltName: Full=Beta-hydroxybutyryl-CoA dehydrogenase;
DE            Short=BHBD;
GN   Name=fadB2; OrderedLocusNames=MT0484;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-hydroxybutanoyl-CoA + NAD(+) = acetoacetyl-CoA + H(+) +
CC         NADH; Xref=Rhea:RHEA:42048, ChEBI:CHEBI:15378, ChEBI:CHEBI:57286,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:78611;
CC         Evidence={ECO:0000250|UniProtKB:P9WNP7};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3S)-3-hydroxybutanoyl-CoA + NADP(+) = acetoacetyl-CoA + H(+)
CC         + NADPH; Xref=Rhea:RHEA:16197, ChEBI:CHEBI:15378, ChEBI:CHEBI:57286,
CC         ChEBI:CHEBI:57316, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.157; Evidence={ECO:0000250|UniProtKB:P9WNP7};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism.
CC   -!- SIMILARITY: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK44708.1; -; Genomic_DNA.
DR   PIR; H70828; H70828.
DR   RefSeq; WP_003402318.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WNP6; -.
DR   SMR; P9WNP6; -.
DR   EnsemblBacteria; AAK44708; AAK44708; MT0484.
DR   GeneID; 45424430; -.
DR   KEGG; mtc:MT0484; -.
DR   PATRIC; fig|83331.31.peg.514; -.
DR   HOGENOM; CLU_009834_2_0_11; -.
DR   UniPathway; UPA00863; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0008691; F:3-hydroxybutyryl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR022694; 3-OHacyl-CoA_DH.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   PIRSF; PIRSF000105; HCDH; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; NADP; Oxidoreductase.
FT   CHAIN           1..286
FT                   /note="3-hydroxybutyryl-CoA dehydrogenase"
FT                   /id="PRO_0000427090"
FT   SITE            143
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   286 AA;  30728 MW;  F1A490B88BCB026B CRC64;
     MSDAIQRVGV VGAGQMGSGI AEVSARAGVE VTVFEPAEAL ITAGRNRIVK SLERAVSAGK
     VTERERDRAL GLLTFTTDLN DLSDRQLVIE AVVEDEAVKS EIFAELDRVV TDPDAVLASN
     TSSIPIMKVA AATKQPQRVL GLHFFNPVPV LPLVELVRTL VTDEAAAART EEFASTVLGK
     QVVRCSDRSG FVVNALLVPY LLSAIRMVEA GFATVEDVDK AVVAGLSHPM GPLRLSDLVG
     LDTLKLIADK MFEEFKEPHY GPPPLLLRMV EAGQLGKKSG RGFYTY