ID   FADD3_COMTE             Reviewed;         540 AA.
AC   Q7WSH3;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl:CoA ligase {ECO:0000303|PubMed:16891113};
DE            Short=HIP:CoA ligase {ECO:0000303|PubMed:16891113};
DE            EC=6.2.1.41 {ECO:0000250|UniProtKB:P96843};
GN   Name=fadD3 {ECO:0000250|UniProtKB:P96843};
GN   Synonyms=ORF18 {ECO:0000303|PubMed:16891113};
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TA441;
RX   PubMed=12902225; DOI=10.1128/aem.69.8.4421-4430.2003;
RA   Horinouchi M., Hayashi T., Yamamoto T., Kudo T.;
RT   "A new bacterial steroid degradation gene cluster in Comamonas testosteroni
RT   TA441 which consists of aromatic-compound degradation genes for seco-
RT   steroids and 3-ketosteroid dehydrogenase genes.";
RL   Appl. Environ. Microbiol. 69:4421-4430(2003).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=TA441;
RX   PubMed=16891113; DOI=10.1016/j.jsbmb.2006.06.006;
RA   Horinouchi M., Hayashi T., Koshino H., Kudo T.;
RT   "ORF18-disrupted mutant of Comamonas testosteroni TA441 accumulates
RT   significant amounts of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid
RT   and its derivatives after incubation with steroids.";
RL   J. Steroid Biochem. Mol. Biol. 101:78-84(2006).
CC   -!- FUNCTION: Involved in the catabolism of the rings C and D of
CC       cholesterol. Catalyzes the ATP-dependent CoA thioesterification of
CC       3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-1,5-indanedione
CC       (HIP) to yield HIP-CoA. {ECO:0000269|PubMed:16891113}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-
CC         yl]propanoate + ATP + CoA = 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-
CC         octahydro-1H-inden-4-yl]propanoyl-CoA + AMP + diphosphate;
CC         Xref=Rhea:RHEA:41640, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:63692, ChEBI:CHEBI:78357,
CC         ChEBI:CHEBI:456215; EC=6.2.1.41;
CC         Evidence={ECO:0000250|UniProtKB:P96843};
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene accumulate 9,17-dioxo-
CC       1,2,3,4,10,19-hexanorandrostan-5-oic acid (HIP) when incubated with
CC       1,4-androstadiene-3,17-dione (ADD). {ECO:0000269|PubMed:16891113}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AB076368; BAC81695.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q7WSH3; -.
DR   SMR; Q7WSH3; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cholesterol metabolism; Ligase; Lipid metabolism;
KW   Nucleotide-binding; Steroid metabolism; Sterol metabolism.
FT   CHAIN           1..540
FT                   /note="3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-
FT                   inden-4-yl]propanoyl:CoA ligase"
FT                   /id="PRO_0000430640"
FT   BINDING         189..197
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         413
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         428
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         519
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   540 AA;  58510 MW;  9E5BC9F9FA421460 CRC64;
     MSKPNQNAMP ESSLTLPGML ADVVSRFAGR AAIVENGKSI SYAQLQQLSR LAARALMSLG
     VQAGDRVALW APNLSEWIVA ACGVHAAGGV LVPLNTRMKG AEAADILERS RARVLVCVGD
     FLNNYYPDLL NGLRPVTLQQ VVVLGDKVLP SADMNWAQFM ARADGTTAEA QQQREARIKP
     DDTADLMFTS GTTGRPKGVM CAHRPTILAF KAWSDVVGLT EGSRYLIVNP FFHTFGYKAG
     WVAALLQGST VYPEQIFDAQ AILHRIESDR ISFLPGPPTL FLSMLAHPGL KNFDLSSLKS
     SVTGASTVPP ILIKRMREEL GIMNVTTAYG LTECGGCATL CEPSDNVETV ANTCGKALPG
     TEVRCVDEQG RPVGPGEAGE VLLRGYHIMQ GYFEDEKATE ETIDADGWLH TGDVGVLDER
     GYLRITDRLK DMFIVGGFNC YPAEIERMLS NHPEVAQVAV VGVADERMGE VGCACVVTRN
     GVTLDQEAFI AWCRANMANY KVPRFVLQLD GLPVNASNKV QKRDLLQIVK DRLASVPQPA