FAKD1_MOUSE
ID FAKD1_MOUSE Reviewed; 829 AA.
AC Q6DI86; A2AR00; B0R0D3; Q5DTU7; Q6IS39; Q8BSV8; Q8C429; Q8R1N6;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=FAST kinase domain-containing protein 1, mitochondrial;
DE Flags: Precursor;
GN Name=Fastkd1; Synonyms=Kiaa1800;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
RC STRAIN=C57BL/6J; TISSUE=Hippocampus, and Pituitary;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Embryo, Eye, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 308-829 (ISOFORM 3).
RC TISSUE=Brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=20869947; DOI=10.1016/j.bbrc.2010.09.075;
RA Simarro M., Gimenez-Cassina A., Kedersha N., Lazaro J.B., Adelmant G.O.,
RA Marto J.A., Rhee K., Tisdale S., Danial N., Benarafa C., Orduna A.,
RA Anderson P.;
RT "Fast kinase domain-containing protein 3 is a mitochondrial protein
RT essential for cellular respiration.";
RL Biochem. Biophys. Res. Commun. 401:440-446(2010).
CC -!- FUNCTION: Involved in the down-regulation of mitochondrial MT-ND3 mRNA
CC levels which leads to decreased respiratory complex I abundance and
CC activity. {ECO:0000250|UniProtKB:Q53R41}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q53R41}.
CC Note=Preferentially localizes to mitochondrial RNA granules, platforms
CC for post-transcriptional RNA modification and ribosome assembly.
CC {ECO:0000250|UniProtKB:Q53R41}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q6DI86-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6DI86-2; Sequence=VSP_024620;
CC Name=3;
CC IsoId=Q6DI86-3; Sequence=VSP_024621;
CC Name=4;
CC IsoId=Q6DI86-4; Sequence=VSP_024618, VSP_024619;
CC -!- TISSUE SPECIFICITY: Expression detected in spleen, testis, colon,
CC heart, smooth muscle, kidney, brain, lung, liver, brown and white
CC adipose tissue with highest expression in heart and brown adipose
CC tissue. {ECO:0000269|PubMed:20869947}.
CC -!- DOMAIN: The RAP domain is essential to regulate MT-ND3 mRNA levels.
CC {ECO:0000250|UniProtKB:Q53R41}.
CC -!- SIMILARITY: Belongs to the FAST kinase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK030411; BAC26950.1; -; mRNA.
DR EMBL; AK083186; BAC38800.1; -; mRNA.
DR EMBL; AL845261; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC023501; AAH23501.1; -; mRNA.
DR EMBL; BC069943; AAH69943.1; -; mRNA.
DR EMBL; BC075689; AAH75689.1; -; mRNA.
DR EMBL; AK220423; BAD90468.1; -; mRNA.
DR CCDS; CCDS16096.1; -. [Q6DI86-1]
DR RefSeq; NP_796218.2; NM_177244.3. [Q6DI86-1]
DR AlphaFoldDB; Q6DI86; -.
DR BioGRID; 236243; 3.
DR STRING; 10090.ENSMUSP00000072896; -.
DR iPTMnet; Q6DI86; -.
DR PhosphoSitePlus; Q6DI86; -.
DR EPD; Q6DI86; -.
DR MaxQB; Q6DI86; -.
DR PaxDb; Q6DI86; -.
DR PRIDE; Q6DI86; -.
DR ProteomicsDB; 271723; -. [Q6DI86-1]
DR ProteomicsDB; 271724; -. [Q6DI86-2]
DR ProteomicsDB; 271725; -. [Q6DI86-3]
DR Antibodypedia; 33812; 142 antibodies from 22 providers.
DR DNASU; 320720; -.
DR Ensembl; ENSMUST00000073152; ENSMUSP00000072896; ENSMUSG00000027086. [Q6DI86-1]
DR Ensembl; ENSMUST00000102706; ENSMUSP00000099767; ENSMUSG00000027086. [Q6DI86-3]
DR GeneID; 320720; -.
DR KEGG; mmu:320720; -.
DR UCSC; uc008jyh.1; mouse. [Q6DI86-1]
DR UCSC; uc008jyi.1; mouse. [Q6DI86-2]
DR UCSC; uc008jyj.1; mouse. [Q6DI86-4]
DR UCSC; uc012bwq.1; mouse. [Q6DI86-3]
DR CTD; 79675; -.
DR MGI; MGI:2444596; Fastkd1.
DR VEuPathDB; HostDB:ENSMUSG00000027086; -.
DR eggNOG; ENOG502QQ64; Eukaryota.
DR GeneTree; ENSGT01030000234607; -.
DR HOGENOM; CLU_017819_0_0_1; -.
DR InParanoid; Q6DI86; -.
DR OMA; HWESNTQ; -.
DR OrthoDB; 352329at2759; -.
DR PhylomeDB; Q6DI86; -.
DR TreeFam; TF324885; -.
DR BioGRID-ORCS; 320720; 5 hits in 72 CRISPR screens.
DR ChiTaRS; Fastkd1; mouse.
DR PRO; PR:Q6DI86; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q6DI86; protein.
DR Bgee; ENSMUSG00000027086; Expressed in interventricular septum and 247 other tissues.
DR Genevisible; Q6DI86; MM.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR GO; GO:0035770; C:ribonucleoprotein granule; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000959; P:mitochondrial RNA metabolic process; ISS:UniProtKB.
DR GO; GO:0000963; P:mitochondrial RNA processing; IBA:GO_Central.
DR GO; GO:0044528; P:regulation of mitochondrial mRNA stability; ISS:UniProtKB.
DR InterPro; IPR013579; FAST_2.
DR InterPro; IPR010622; FAST_Leu-rich.
DR InterPro; IPR013584; RAP.
DR Pfam; PF06743; FAST_1; 1.
DR Pfam; PF08368; FAST_2; 1.
DR Pfam; PF08373; RAP; 1.
DR SMART; SM00952; RAP; 1.
DR PROSITE; PS51286; RAP; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Alternative splicing; Mitochondrion; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000305"
FT CHAIN ?..829
FT /note="FAST kinase domain-containing protein 1,
FT mitochondrial"
FT /id="PRO_0000284711"
FT DOMAIN 761..821
FT /note="RAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00619"
FT MOD_RES 346
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q53R41"
FT VAR_SEQ 136..160
FT /note="FDTNVLSIFSTCLADQHLYFSPLMG -> QVCWACHRRSGLFCGRLSRWNIG
FT KE (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024618"
FT VAR_SEQ 161..829
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024619"
FT VAR_SEQ 348..829
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024620"
FT VAR_SEQ 524..552
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_024621"
FT CONFLICT 56
FT /note="C -> W (in Ref. 1; BAC26950)"
FT /evidence="ECO:0000305"
FT CONFLICT 73
FT /note="K -> N (in Ref. 1; BAC26950)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="L -> I (in Ref. 1; BAC38800)"
FT /evidence="ECO:0000305"
FT CONFLICT 443
FT /note="Y -> C (in Ref. 4; BAD90468)"
FT /evidence="ECO:0000305"
FT CONFLICT 471
FT /note="Q -> H (in Ref. 4; BAD90468)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 829 AA; 95398 MW; 71325AABBF21EA30 CRC64;
MFRLRSISLL SWRAFPLRPF SCESLITQMQ KCTNEEQVFD LIETNTATLS EQQVGCAFNV
LWQFQKQKTV LEKNVDHVRN HPQFLTLCSI TTNHIPAMSD ATLVDVLYSI KQFAVESHHP
LIEALVTEAW KRLERFDTNV LSIFSTCLAD QHLYFSPLMG KIADIVNRRL ETIQDLRALS
VLMVSISSLI SPCFQERLVI RTELLFDTVN SSKVNIARRI LLFLRNVKYS HYPLLERCNQ
VFIRNMSHLD LESISKILNL YQFLQFHSFE FVEAARGRLA EMTLPSDHPE SFVRLFAALG
PVARPEIKKQ LKSTILLLSE ELSSQQALIV LGAMEDMESR NSHLLKKIVS VLYKHLDNYK
SIELLKIIQA LTFLHFQSKE LFMKLRELLL SRLEASVIPS EISVLVSALS MLPHPHLSET
AVSRIEAVLP QCDFRELNDL VVYLMRWIQS DLVCLASTTG KQLDLLQKLD QLGRHRLQQS
TNLDLLWEEL KSLKGEWLHE SLVEESIAAL LRFMDEIDYS NIAKVASFLS RTNYLNTLLL
DRIASVAIQQ VEKIHPFSVL AIILPFSILN YDPPQKDEFF GACVQCCNSY LGTLDPGTLV
FLGFSLAVLE YFPEDLLKKM FNIEFLARLD SQLEILPSSL SARIQFRLME LNRAVCLECP
ELQVPWFHDR FCQRQFNKDT GVMNGAQQQI YKMLAEVLGG HQCVKPSALS PYYHTVGFEC
ILDKRKKPLP YESHSIAPRK SLGMHWDSRV EPRLPPEAER IAIELLDVRA FCSNIPHLKG
KSAMKKRHLE ILGYRVIQIP YFEWNSMAMS TKDARMDYLR EHLFGEGKS
