FAKD3_MOUSE
ID FAKD3_MOUSE Reviewed; 661 AA.
AC Q8BSN9; Q5XKD3; Q6P9N4; Q8CB52; Q8CCG7;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=FAST kinase domain-containing protein 3, mitochondrial;
DE Flags: Precursor;
GN Name=Fastkd3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Forelimb, Testis, and Vagina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=20869947; DOI=10.1016/j.bbrc.2010.09.075;
RA Simarro M., Gimenez-Cassina A., Kedersha N., Lazaro J.B., Adelmant G.O.,
RA Marto J.A., Rhee K., Tisdale S., Danial N., Benarafa C., Orduna A.,
RA Anderson P.;
RT "Fast kinase domain-containing protein 3 is a mitochondrial protein
RT essential for cellular respiration.";
RL Biochem. Biophys. Res. Commun. 401:440-446(2010).
CC -!- FUNCTION: Required for normal mitochondrial respiration. Increases
CC steady-state levels and half-lives of a subset of mature mitochondrial
CC mRNAs MT-ND2, MT-ND3, MT-CYTB, MT-CO2, and MT-ATP8/6. Promotes MT-CO1
CC mRNA translation and increases mitochondrial complex IV assembly and
CC activity. {ECO:0000250|UniProtKB:Q14CZ7}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q14CZ7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8BSN9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BSN9-2; Sequence=VSP_024625, VSP_024626;
CC Name=3;
CC IsoId=Q8BSN9-3; Sequence=VSP_024624;
CC -!- TISSUE SPECIFICITY: Expression detected in spleen, testis, colon,
CC heart, smooth muscle, kidney, brain, lung, liver, brown and white
CC adipose tissue with highest expression in testis and smooth muscle.
CC {ECO:0000269|PubMed:20869947}.
CC -!- DOMAIN: RAP domain is required for FASTKD3 function in mRNA stability
CC and translation. {ECO:0000250|UniProtKB:Q14CZ7}.
CC -!- SIMILARITY: Belongs to the FAST kinase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC27277.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK031142; BAC27277.1; ALT_FRAME; mRNA.
DR EMBL; AK033189; BAC28191.1; -; mRNA.
DR EMBL; AK036778; BAC29573.1; -; mRNA.
DR EMBL; BC042506; AAH42506.1; -; mRNA.
DR EMBL; BC060688; AAH60688.1; -; mRNA.
DR CCDS; CCDS36719.1; -. [Q8BSN9-1]
DR RefSeq; NP_001317352.1; NM_001330423.1. [Q8BSN9-1]
DR RefSeq; NP_081399.3; NM_027123.5. [Q8BSN9-1]
DR AlphaFoldDB; Q8BSN9; -.
DR SMR; Q8BSN9; -.
DR STRING; 10090.ENSMUSP00000061737; -.
DR iPTMnet; Q8BSN9; -.
DR PhosphoSitePlus; Q8BSN9; -.
DR EPD; Q8BSN9; -.
DR PaxDb; Q8BSN9; -.
DR PeptideAtlas; Q8BSN9; -.
DR PRIDE; Q8BSN9; -.
DR ProteomicsDB; 271858; -. [Q8BSN9-1]
DR ProteomicsDB; 271859; -. [Q8BSN9-2]
DR ProteomicsDB; 271860; -. [Q8BSN9-3]
DR Antibodypedia; 22426; 190 antibodies from 28 providers.
DR DNASU; 69577; -.
DR Ensembl; ENSMUST00000051784; ENSMUSP00000061737; ENSMUSG00000021532. [Q8BSN9-1]
DR Ensembl; ENSMUST00000223319; ENSMUSP00000152635; ENSMUSG00000021532. [Q8BSN9-2]
DR GeneID; 69577; -.
DR KEGG; mmu:69577; -.
DR UCSC; uc007rca.1; mouse. [Q8BSN9-1]
DR CTD; 79072; -.
DR MGI; MGI:1916827; Fastkd3.
DR VEuPathDB; HostDB:ENSMUSG00000021532; -.
DR eggNOG; ENOG502QW8P; Eukaryota.
DR GeneTree; ENSGT01030000234607; -.
DR HOGENOM; CLU_028858_0_0_1; -.
DR InParanoid; Q8BSN9; -.
DR OMA; VQIPYHE; -.
DR OrthoDB; 352329at2759; -.
DR PhylomeDB; Q8BSN9; -.
DR TreeFam; TF324885; -.
DR BioGRID-ORCS; 69577; 5 hits in 74 CRISPR screens.
DR ChiTaRS; Fastkd3; mouse.
DR PRO; PR:Q8BSN9; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q8BSN9; protein.
DR Bgee; ENSMUSG00000021532; Expressed in cleaving embryo and 209 other tissues.
DR ExpressionAtlas; Q8BSN9; baseline and differential.
DR Genevisible; Q8BSN9; MM.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0035770; C:ribonucleoprotein granule; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; ISS:UniProtKB.
DR GO; GO:0000963; P:mitochondrial RNA processing; IBA:GO_Central.
DR GO; GO:0070131; P:positive regulation of mitochondrial translation; ISS:UniProtKB.
DR GO; GO:0044528; P:regulation of mitochondrial mRNA stability; ISS:UniProtKB.
DR InterPro; IPR013579; FAST_2.
DR InterPro; IPR010622; FAST_Leu-rich.
DR InterPro; IPR013584; RAP.
DR Pfam; PF06743; FAST_1; 1.
DR Pfam; PF08368; FAST_2; 1.
DR Pfam; PF08373; RAP; 1.
DR SMART; SM00952; RAP; 1.
DR PROSITE; PS51286; RAP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Mitochondrion; Reference proteome; Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000305"
FT CHAIN ?..661
FT /note="FAST kinase domain-containing protein 3,
FT mitochondrial"
FT /id="PRO_0000284716"
FT DOMAIN 592..650
FT /note="RAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00619"
FT VAR_SEQ 510..661
FT /note="GPKLLPKYQVKSFLTPCCSLETPLDLHLYKSVVIGLIDLLGSRLYFASKVLT
FT PYYYTIDVEVKLDEDGFVLPCTVDEDIHKRVALCIDGPQRFCLDSKHLLGKEATKQRHL
FT RLLGYQVVQLPYHELELLTSRLELVDYLQRKLFSQSSAVHW -> VRTETRVV (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_024624"
FT VAR_SEQ 568..571
FT /note="DVEV -> ASAL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_024625"
FT VAR_SEQ 572..661
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_024626"
FT CONFLICT 34
FT /note="P -> Q (in Ref. 2; AAH42506)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="Y -> V (in Ref. 2; AAH42506)"
FT /evidence="ECO:0000305"
FT CONFLICT 175
FT /note="N -> S (in Ref. 2; AAH42506)"
FT /evidence="ECO:0000305"
FT CONFLICT 258
FT /note="T -> A (in Ref. 2; AAH42506)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="V -> A (in Ref. 2; AAH42506)"
FT /evidence="ECO:0000305"
FT CONFLICT 276
FT /note="M -> T (in Ref. 2; AAH42506)"
FT /evidence="ECO:0000305"
FT CONFLICT 533
FT /note="L -> M (in Ref. 2; AAH42506)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 661 AA; 75317 MW; 67A53D7BFD52AB74 CRC64;
MAFITLRRAF CHKSILWIPG AVVALKIHPA SHAPKAVTDR LSVCFCSLQP ELFRVRFHHA
YCKNFHSEKG NDFHPVGEPW SSQAQEWNQP GQSLQNEDEE MLFRRLSYFT SFEEVLSFIS
ALDTLPVPLA MAALLRICEI GRRDGEQRLP EGVLENRAFQ ALCLRCERDP SHLTNAGLVT
ALQSLLTLLP ADPQSSLMLS LVAECQRRLQ RGNLEVHHLC VLGESLAMLQ GASCETLKLV
VRQLQSKSVE TFAPEEITSV YRILQVCPEE VDKHQMFLNT LNNFSISVVP YLSPKSISHV
LTALVALDQT HALPLLIKLG KYVVRYIPRF TNEELRKVLE AFVYFGHSDR FFTEALEQHV
SALCFSLDPA VASSVMGYCS RKRILSKPIF DVVSEIVVCQ WDRLSPSQIA ELIEPFGKLN
YVPPNAPALF RKVENVLCAR LHHFPPKMLL RLLHSCALIE RHPVNFMSKL FSPFFLQRLQ
GKESYLDRLS LAQLTQLFLT SVLECPFYKG PKLLPKYQVK SFLTPCCSLE TPLDLHLYKS
VVIGLIDLLG SRLYFASKVL TPYYYTIDVE VKLDEDGFVL PCTVDEDIHK RVALCIDGPQ
RFCLDSKHLL GKEATKQRHL RLLGYQVVQL PYHELELLTS RLELVDYLQR KLFSQSSAVH
W
