FAKD5_HUMAN
ID FAKD5_HUMAN Reviewed; 764 AA.
AC Q7L8L6; Q96JN3; Q9H5D1; Q9H8Y3;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=FAST kinase domain-containing protein 5, mitochondrial;
DE Flags: Precursor;
GN Name=FASTKD5; Synonyms=KIAA1792;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=11347906; DOI=10.1093/dnares/8.2.85;
RA Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XX. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 8:85-95(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=16964243; DOI=10.1038/nbt1240;
RA Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
RT "A probability-based approach for high-throughput protein phosphorylation
RT analysis and site localization.";
RL Nat. Biotechnol. 24:1285-1292(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-507, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [9]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=20869947; DOI=10.1016/j.bbrc.2010.09.075;
RA Simarro M., Gimenez-Cassina A., Kedersha N., Lazaro J.B., Adelmant G.O.,
RA Marto J.A., Rhee K., Tisdale S., Danial N., Benarafa C., Orduna A.,
RA Anderson P.;
RT "Fast kinase domain-containing protein 3 is a mitochondrial protein
RT essential for cellular respiration.";
RL Biochem. Biophys. Res. Commun. 401:440-446(2010).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [12]
RP FUNCTION, IDENTIFICATION IN A COMPLEX WITH GRSF1; DDX28; DHX30 AND FASTKD2,
RP SUBCELLULAR LOCATION, RNA-BINDING, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=25683715; DOI=10.1016/j.celrep.2015.01.030;
RA Antonicka H., Shoubridge E.A.;
RT "Mitochondrial RNA granules are centers for post-transcriptional RNA
RT processing and ribosome biogenesis.";
RL Cell Rep. 10:920-932(2015).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: Plays an important role in the processing of non-canonical
CC mitochondrial mRNA precursors (PubMed:25683715).
CC {ECO:0000269|PubMed:25683715}.
CC -!- SUBUNIT: Found in a complex with GRSF1, DDX28, DHX30 and FASTKD2.
CC Associates with the 12S mitochondrial rRNA (12S mt-rRNA).
CC {ECO:0000269|PubMed:25683715}.
CC -!- INTERACTION:
CC Q7L8L6; O95259: KCNH1; NbExp=3; IntAct=EBI-747570, EBI-2909270;
CC Q7L8L6; Q9UJV3-2: MID2; NbExp=3; IntAct=EBI-747570, EBI-10172526;
CC Q7L8L6; Q14160: SCRIB; NbExp=2; IntAct=EBI-747570, EBI-357345;
CC Q7L8L6; P14373: TRIM27; NbExp=3; IntAct=EBI-747570, EBI-719493;
CC Q7L8L6; P61086: UBE2K; NbExp=3; IntAct=EBI-747570, EBI-473850;
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix, mitochondrion nucleoid
CC {ECO:0000269|PubMed:20869947, ECO:0000269|PubMed:25683715}.
CC Note=Localizes to mitochondrial RNA granules found in close proximity
CC to the mitochondrial nucleoids. {ECO:0000269|PubMed:25683715}.
CC -!- TISSUE SPECIFICITY: Expression detected in spleen, thymus, testis,
CC ovary, colon, heart, smooth muscle, kidney, brain, lung, liver and
CC white adipose tissue with highest expression in heart, smooth muscle,
CC liver and thyroid. {ECO:0000269|PubMed:20869947}.
CC -!- SIMILARITY: Belongs to the FAST kinase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB15696.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAB47421.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB058695; BAB47421.1; ALT_INIT; mRNA.
DR EMBL; AK023211; BAB14464.1; -; mRNA.
DR EMBL; AK027215; BAB15696.1; ALT_FRAME; mRNA.
DR EMBL; AL121891; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC011856; AAH11856.1; -; mRNA.
DR EMBL; BC007413; AAH07413.1; -; mRNA.
DR CCDS; CCDS13048.1; -.
DR RefSeq; NP_068598.1; NM_021826.4.
DR AlphaFoldDB; Q7L8L6; -.
DR BioGRID; 121924; 313.
DR CORUM; Q7L8L6; -.
DR IntAct; Q7L8L6; 31.
DR MINT; Q7L8L6; -.
DR STRING; 9606.ENSP00000369618; -.
DR GlyGen; Q7L8L6; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q7L8L6; -.
DR PhosphoSitePlus; Q7L8L6; -.
DR SwissPalm; Q7L8L6; -.
DR BioMuta; FASTKD5; -.
DR DMDM; 74749905; -.
DR EPD; Q7L8L6; -.
DR jPOST; Q7L8L6; -.
DR MassIVE; Q7L8L6; -.
DR MaxQB; Q7L8L6; -.
DR PaxDb; Q7L8L6; -.
DR PeptideAtlas; Q7L8L6; -.
DR PRIDE; Q7L8L6; -.
DR ProteomicsDB; 68838; -.
DR Antibodypedia; 23456; 99 antibodies from 19 providers.
DR DNASU; 60493; -.
DR Ensembl; ENST00000380266.4; ENSP00000369618.3; ENSG00000215251.5.
DR Ensembl; ENST00000687419.1; ENSP00000510780.1; ENSG00000215251.5.
DR Ensembl; ENST00000688674.1; ENSP00000510078.1; ENSG00000215251.5.
DR Ensembl; ENST00000692371.1; ENSP00000510038.1; ENSG00000215251.5.
DR GeneID; 60493; -.
DR KEGG; hsa:60493; -.
DR MANE-Select; ENST00000380266.4; ENSP00000369618.3; NM_021826.5; NP_068598.1.
DR UCSC; uc002whz.5; human.
DR CTD; 60493; -.
DR DisGeNET; 60493; -.
DR GeneCards; FASTKD5; -.
DR HGNC; HGNC:25790; FASTKD5.
DR HPA; ENSG00000215251; Low tissue specificity.
DR neXtProt; NX_Q7L8L6; -.
DR OpenTargets; ENSG00000215251; -.
DR PharmGKB; PA145148886; -.
DR VEuPathDB; HostDB:ENSG00000215251; -.
DR eggNOG; ENOG502QRPY; Eukaryota.
DR GeneTree; ENSGT01030000234607; -.
DR HOGENOM; CLU_021040_0_0_1; -.
DR InParanoid; Q7L8L6; -.
DR OMA; KRMILPH; -.
DR OrthoDB; 452836at2759; -.
DR PhylomeDB; Q7L8L6; -.
DR TreeFam; TF352874; -.
DR PathwayCommons; Q7L8L6; -.
DR SignaLink; Q7L8L6; -.
DR SIGNOR; Q7L8L6; -.
DR BioGRID-ORCS; 60493; 216 hits in 1085 CRISPR screens.
DR GenomeRNAi; 60493; -.
DR Pharos; Q7L8L6; Tdark.
DR PRO; PR:Q7L8L6; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; Q7L8L6; protein.
DR Bgee; ENSG00000215251; Expressed in gastrocnemius and 195 other tissues.
DR Genevisible; Q7L8L6; HS.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0042645; C:mitochondrial nucleoid; IDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0035770; C:ribonucleoprotein granule; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0019843; F:rRNA binding; IDA:UniProtKB.
DR GO; GO:0000963; P:mitochondrial RNA processing; IMP:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0044528; P:regulation of mitochondrial mRNA stability; IBA:GO_Central.
DR InterPro; IPR013579; FAST_2.
DR InterPro; IPR010622; FAST_Leu-rich.
DR InterPro; IPR013584; RAP.
DR Pfam; PF06743; FAST_1; 1.
DR Pfam; PF08368; FAST_2; 1.
DR Pfam; PF08373; RAP; 1.
DR SMART; SM00952; RAP; 1.
DR PROSITE; PS51286; RAP; 1.
PE 1: Evidence at protein level;
KW Acetylation; Mitochondrion; Mitochondrion nucleoid; mRNA processing;
KW Phosphoprotein; Reference proteome; RNA-binding; rRNA-binding;
KW Transit peptide.
FT TRANSIT 1..27
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 28..764
FT /note="FAST kinase domain-containing protein 5,
FT mitochondrial"
FT /id="PRO_0000284979"
FT DOMAIN 697..757
FT /note="RAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00619"
FT MOD_RES 95
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:16964243,
FT ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 507
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:19608861"
FT VARIANT 256
FT /note="R -> C (in dbSNP:rs3746700)"
FT /id="VAR_053891"
FT VARIANT 288
FT /note="I -> T (in dbSNP:rs2422857)"
FT /id="VAR_053892"
FT VARIANT 377
FT /note="I -> V (in dbSNP:rs3746699)"
FT /id="VAR_053893"
SQ SEQUENCE 764 AA; 86574 MW; 1064229F9B212405 CRC64;
MAATLKSLKL VRYRAFCSPS AFGAVRSVSY WNVSSTQHGG QDPPEHISLC HSAKKVKNIC
STFSSRRILT TSSAHPGLEF SKTSSSKAST LQLGSPRATG VDEEDVEVFD SFENMRVFLQ
LRPEYRVHSY NASETSQLLS VSEGELILHK VRVNQNNLQA QVIVDYLCKL SSLPAEQHPV
LLGSTSFALL CQLSVKKIQL FDTQDLINVL KAFVILGIPH SHSMLDVYET KCCHQVWEMN
MDQLLLVADL WRYLGRKVPR FLNIFSSYLN LHWKDLSLSQ LVHLIYVIGE NRQVSQDLMQ
KLESLILKYI DLINLEEVGT ICLGFFKSST NLSEFVMRKI GDLACANIQH LSSRSLVNIV
KMFRFTHVDH INFMKQIGEI APQRIPSLGV QGVMHLTLYC SALRFLNEGV MNAVAASLPP
RVAHCRSKDV AKILWSFGTL NYKPPNAEEF YSSLISEIHR KMPEFNQYPE HLPTCLLGLA
FLEYFPVELI DFALSPGFVR LAQERTKFDL LKELYTLDGT VGIECPDYRG NRLSTHLQQE
GSELLWYLAE KDMNSKPEFL ETVFLLETML GGPQYVKHHM ILPHTRSSDL EVQLDVNLKP
LPFNREATPA ENVAKLRLEH VGVSLTDDLM NKLLKGKARG HFQGKTESEP GQQPMELENK
AAVPLGGFLC NVADKSGAME MAGLCPAACM QTPRMKLAVQ FTNRNQYCYG SRDLLGLHNM
KRRQLARLGY RVVELSYWEW LPLLKRTRLE KLAFLHEKVF TSAL
