FAL1_CANAL
ID FAL1_CANAL Reviewed; 399 AA.
AC Q5A9Z6; A0A1D8PDK9; Q9UVI5;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=ATP-dependent RNA helicase FAL1;
DE EC=3.6.4.13;
GN Name=FAL1; OrderedLocusNames=CAALFM_C105640CA;
GN ORFNames=CaO19.10024, CaO19.2488;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=B2630;
RA De Backer M.D., Logghe M., Viaene J., Loonen I., Vandoninck S.,
RA de Hoogt R., Nelissen B., De Waele S., Simons F., Contreras R., Luyten W.;
RT "A novel method for systematic identification of genes required for growth
RT of Candida albicans.";
RL Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- FUNCTION: ATP-dependent RNA helicase involved in 40S ribosomal subunit
CC biogenesis. Required for the processing and cleavage of 35S pre-rRNA at
CC sites A0, A1, and A2, leading to mature 18S rRNA (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX48/FAL1
CC subfamily. {ECO:0000305}.
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DR EMBL; AJ250879; CAB61567.1; -; Genomic_DNA.
DR EMBL; CP017623; AOW26229.1; -; Genomic_DNA.
DR RefSeq; XP_718509.1; XM_713416.1.
DR AlphaFoldDB; Q5A9Z6; -.
DR SMR; Q5A9Z6; -.
DR STRING; 237561.Q5A9Z6; -.
DR GeneID; 3639839; -.
DR KEGG; cal:CAALFM_C105640CA; -.
DR CGD; CAL0000189056; FAL1.
DR VEuPathDB; FungiDB:C1_05640C_A; -.
DR eggNOG; KOG0328; Eukaryota.
DR HOGENOM; CLU_003041_1_0_1; -.
DR InParanoid; Q5A9Z6; -.
DR OrthoDB; 726081at2759; -.
DR PRO; PR:Q5A9Z6; -.
DR Proteomes; UP000000559; Chromosome 1.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0097078; C:FAL1-SGD1 complex; IEA:EnsemblFungi.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0030688; C:preribosome, small subunit precursor; IEA:EnsemblFungi.
DR GO; GO:0032040; C:small-subunit processome; IEA:EnsemblFungi.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR GO; GO:0032774; P:RNA biosynthetic process; IEA:EnsemblFungi.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT CHAIN 1..399
FT /note="ATP-dependent RNA helicase FAL1"
FT /id="PRO_0000232143"
FT DOMAIN 57..227
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 238..399
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 26..54
FT /note="Q motif"
FT MOTIF 175..178
FT /note="DEAD box"
FT BINDING 70..77
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 48
FT /note="T -> A (in Ref. 1; CAB61567)"
FT /evidence="ECO:0000305"
FT CONFLICT 115
FT /note="K -> M (in Ref. 1; CAB61567)"
FT /evidence="ECO:0000305"
FT CONFLICT 380
FT /note="F -> L (in Ref. 1; CAB61567)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 399 AA; 45555 MW; BA004123E1196DF1 CRC64;
MDDFDRDLDN ELEFSHKSTK GIKVHRTFES MNLKPDLLKG IYAYGFETPS AIQSRAIMQI
ISGRDTIAQA QSGTGKTATF SIGMLEVIDT KSKECQALIL SPTRELAIQI QNVVKHLGDY
MNIHTHACIG GKNVGEDVKK LQQGQQIVSG TPGRVIDVIK RRNLQTRNIK VLILDEADEL
FTKGFKEQIY EIYKHLPPSV QVVVVSATLP REVLEMTSKF TTDPVKILVK RDEISLSGIK
QYYVQCERED WKFDTLCDLY DNLTITQAVI FCNTKLKVNW LADQMKKQNF TVVAMHGDMK
QDERDSIMND FRRGNSRVLI STDVWARGID VQQVSLVINY DLPTDKENYI HRIGRSGRFG
RKGTAINLIT KDDVVTLKEF EKYYSTKIKE MPMNINDIM
