ID   FAL1_PICST              Reviewed;         399 AA.
AC   A3GFV3;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=ATP-dependent RNA helicase FAL1;
DE            EC=3.6.4.13;
GN   Name=FAL1; ORFNames=PICST_52941;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: ATP-dependent RNA helicase involved in 40S ribosomal subunit
CC       biogenesis. Required for the processing and cleavage of 35S pre-rRNA at
CC       sites A0, A1, and A2, leading to mature 18S rRNA (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX48/FAL1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AAVQ01000001; EAZ63408.1; -; Genomic_DNA.
DR   RefSeq; XP_001387431.1; XM_001387394.1.
DR   AlphaFoldDB; A3GFV3; -.
DR   SMR; A3GFV3; -.
DR   STRING; 4924.XP_001387431.1; -.
DR   EnsemblFungi; EAZ63408; EAZ63408; PICST_52941.
DR   GeneID; 4851152; -.
DR   KEGG; pic:PICST_52941; -.
DR   eggNOG; KOG0328; Eukaryota.
DR   HOGENOM; CLU_003041_1_0_1; -.
DR   InParanoid; A3GFV3; -.
DR   OMA; HLGDYMN; -.
DR   OrthoDB; 726081at2759; -.
DR   Proteomes; UP000002258; Chromosome 1.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW   Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT   CHAIN           1..399
FT                   /note="ATP-dependent RNA helicase FAL1"
FT                   /id="PRO_0000285136"
FT   DOMAIN          57..227
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          238..399
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           26..54
FT                   /note="Q motif"
FT   MOTIF           175..178
FT                   /note="DEAD box"
FT   BINDING         70..77
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   399 AA;  45461 MW;  174DB41B7614FCA9 CRC64;
     MDDFDRDLDN ELEFSHKSTK NVKVHATFES MNLKPDLLKG IYGYGFEAPS AIQSRAIMQI
     INGRDTIAQA QSGTGKTATF SIGMLQAIDT NAKDCQALIL SPTRELAVQI QNVVKHLGDY
     MNIHTHACIG GTHVGDDIKK LKQGQQIVSG TPGRVVDMVK RQQLSTRNIK MLILDEADEL
     FTKGFKEQIY EVYKYLPPSV QVVVVSATLS REVLEMTNKF TTDPVKILVK RDQITLEGIK
     QYHVQCEKED WKFDTLCDLY DNLTITQAVI FCNTKLKVNW LTDQMRKQNF TVVSMHGDMK
     QDERDSIMND FRTGNSRVLI STDVWARGID VQQVSLVINY DLPTDKENYI HRIGRSGRFG
     RKGVAINLIT KEDVATLRDF EKYYSTKIRE MPMNINDIM