FAM3B_HUMAN
ID FAM3B_HUMAN Reviewed; 235 AA.
AC P58499;
DT 19-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Protein FAM3B;
DE AltName: Full=Cytokine-like protein 2-21;
DE AltName: Full=Pancreatic-derived factor;
DE Short=PANDER;
DE Flags: Precursor;
GN Name=FAM3B; Synonyms=C21orf11, C21orf76; ORFNames=PRED44, UNQ320/PRO365;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C).
RX PubMed=11707072; DOI=10.1006/geno.2001.6640;
RA Reymond A., Friedli M., Neergaard Henrichsen C., Chapot F., Deutsch S.,
RA Ucla C., Rossier C., Lyle R., Guipponi M., Antonarakis S.E.;
RT "From PREDs and open reading frames to cDNA isolation: revisiting the human
RT chromosome 21 transcription map.";
RL Genomics 78:46-54(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), PARTIAL PROTEIN SEQUENCE, AND
RP CHARACTERIZATION.
RX PubMed=12160727; DOI=10.1006/geno.2002.6816;
RA Zhu Y., Xu G., Patel A., McLaughlin M.M., Silverman C., Knecht K.A.,
RA Sweitzer S., Li X., McDonnell P., Mirabile R., Zimmerman D., Boyce R.,
RA Tierney L.A., Hu E., Livi G.P., Wolf B.A., Abdel-Meguid S.S., Rose G.D.,
RA Aurora R., Hensley P., Briggs M., Young P.R.;
RT "Cloning, expression, and initial characterization of a novel cytokine-like
RT gene family.";
RL Genomics 80:144-150(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DISULFIDE BONDS.
RX PubMed=16114871; DOI=10.1021/bi0503908;
RA Yang J., Gao Z., Robert C.E., Burkhardt B.R., Gaweska H., Wagner A., Wu J.,
RA Greene S.R., Young R.A., Wolf B.A.;
RT "Structure-function studies of PANDER, an islet specific cytokine inducing
RT cell death of insulin-secreting beta cells.";
RL Biochemistry 44:11342-11352(2005).
CC -!- FUNCTION: Induces apoptosis of alpha and beta cells in a dose- and
CC time-dependent manner. {ECO:0000269|PubMed:16114871}.
CC -!- INTERACTION:
CC P58499; P21217: FUT3; NbExp=3; IntAct=EBI-12955347, EBI-12839380;
CC P58499; Q53GS7: GLE1; NbExp=3; IntAct=EBI-12955347, EBI-1955541;
CC P58499; P02545: LMNA; NbExp=3; IntAct=EBI-12955347, EBI-351935;
CC -!- SUBCELLULAR LOCATION: Secreted. Note=Present in insulin secretory
CC granules and likely cosecreted with insulin. Localized in discrete
CC vesicular and perinuclear structure.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=B;
CC IsoId=P58499-1; Sequence=Displayed;
CC Name=A;
CC IsoId=P58499-2; Sequence=VSP_001504;
CC Name=C;
CC IsoId=P58499-3; Sequence=VSP_001505;
CC -!- TISSUE SPECIFICITY: Highly expressed in the pancreas. Also found in the
CC colon, kidney, prostate, small intestine and testis.
CC {ECO:0000269|PubMed:16114871}.
CC -!- PTM: 2 N-termini have been observed in the mature protein: the first at
CC Glu-30, resulting from signal peptide cleavage, the second at Ser-46.
CC -!- PTM: O-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the FAM3 family. {ECO:0000305}.
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DR EMBL; AJ409094; CAC83974.1; -; mRNA.
DR EMBL; AF375989; AAL34495.1; -; mRNA.
DR EMBL; AY040086; AAK74134.1; -; mRNA.
DR EMBL; AF494379; AAM94280.1; -; mRNA.
DR EMBL; AY358459; AAQ88824.1; -; mRNA.
DR EMBL; BC057829; AAH57829.1; -; mRNA.
DR CCDS; CCDS13671.1; -. [P58499-1]
DR CCDS; CCDS42930.1; -. [P58499-3]
DR RefSeq; NP_478066.3; NM_058186.3. [P58499-1]
DR RefSeq; NP_996847.1; NM_206964.1. [P58499-3]
DR AlphaFoldDB; P58499; -.
DR SMR; P58499; -.
DR BioGRID; 119895; 13.
DR IntAct; P58499; 11.
DR STRING; 9606.ENSP00000350673; -.
DR GlyGen; P58499; 2 sites.
DR iPTMnet; P58499; -.
DR PhosphoSitePlus; P58499; -.
DR BioMuta; FAM3B; -.
DR jPOST; P58499; -.
DR MassIVE; P58499; -.
DR PaxDb; P58499; -.
DR PeptideAtlas; P58499; -.
DR PRIDE; P58499; -.
DR ProteomicsDB; 57077; -. [P58499-1]
DR ProteomicsDB; 57078; -. [P58499-2]
DR ProteomicsDB; 57079; -. [P58499-3]
DR Antibodypedia; 2683; 311 antibodies from 29 providers.
DR DNASU; 54097; -.
DR Ensembl; ENST00000357985.7; ENSP00000350673.2; ENSG00000183844.17. [P58499-1]
DR Ensembl; ENST00000398647.7; ENSP00000381642.3; ENSG00000183844.17. [P58499-3]
DR Ensembl; ENST00000398652.7; ENSP00000381646.3; ENSG00000183844.17. [P58499-2]
DR GeneID; 54097; -.
DR KEGG; hsa:54097; -.
DR MANE-Select; ENST00000357985.7; ENSP00000350673.2; NM_058186.4; NP_478066.3.
DR UCSC; uc002yzb.2; human. [P58499-1]
DR CTD; 54097; -.
DR DisGeNET; 54097; -.
DR GeneCards; FAM3B; -.
DR HGNC; HGNC:1253; FAM3B.
DR HPA; ENSG00000183844; Tissue enhanced (intestine, pancreas, salivary gland, stomach).
DR MIM; 608617; gene.
DR neXtProt; NX_P58499; -.
DR OpenTargets; ENSG00000183844; -.
DR PharmGKB; PA27979; -.
DR VEuPathDB; HostDB:ENSG00000183844; -.
DR eggNOG; ENOG502QW7Y; Eukaryota.
DR GeneTree; ENSGT00950000183004; -.
DR HOGENOM; CLU_099478_1_1_1; -.
DR InParanoid; P58499; -.
DR OMA; FASVCAW; -.
DR OrthoDB; 1295040at2759; -.
DR PhylomeDB; P58499; -.
DR TreeFam; TF353414; -.
DR PathwayCommons; P58499; -.
DR SignaLink; P58499; -.
DR BioGRID-ORCS; 54097; 10 hits in 1069 CRISPR screens.
DR ChiTaRS; FAM3B; human.
DR GeneWiki; FAM3B; -.
DR GenomeRNAi; 54097; -.
DR Pharos; P58499; Tbio.
DR PRO; PR:P58499; -.
DR Proteomes; UP000005640; Chromosome 21.
DR RNAct; P58499; protein.
DR Bgee; ENSG00000183844; Expressed in ileal mucosa and 128 other tissues.
DR ExpressionAtlas; P58499; baseline and differential.
DR Genevisible; P58499; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005576; C:extracellular region; NAS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; NAS:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0042593; P:glucose homeostasis; IEA:InterPro.
DR GO; GO:0030073; P:insulin secretion; IDA:UniProtKB.
DR InterPro; IPR039220; FAM3.
DR InterPro; IPR039214; FAM3B.
DR InterPro; IPR039477; ILEI/PANDER_dom.
DR PANTHER; PTHR14592; PTHR14592; 1.
DR PANTHER; PTHR14592:SF2; PTHR14592:SF2; 1.
DR Pfam; PF15711; ILEI; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Cytokine; Direct protein sequencing;
KW Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT CHAIN 30..235
FT /note="Protein FAM3B"
FT /id="PRO_0000008750"
FT CARBOHYD 120
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 208
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 63..91
FT /evidence="ECO:0000250"
FT DISULFID 69..229
FT /evidence="ECO:0000250"
FT VAR_SEQ 7..54
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000303|PubMed:11707072"
FT /id="VSP_001505"
FT VAR_SEQ 7
FT /note="G -> GSRWACWLTRCLISCFDINVQGRLLVKLRPKPTANTTCPG (in
FT isoform A)"
FT /evidence="ECO:0000303|PubMed:11707072"
FT /id="VSP_001504"
FT VARIANT 14
FT /note="V -> M (in dbSNP:rs2838012)"
FT /id="VAR_021953"
SQ SEQUENCE 235 AA; 25982 MW; 05B74722D03155FF CRC64;
MRPLAGGLLK VVFVVFASLC AWYSGYLLAE LIPDAPLSSA AYSIRSIGER PVLKAPVPKR
QKCDHWTPCP SDTYAYRLLS GGGRSKYAKI CFEDNLLMGE QLGNVARGIN IAIVNYVTGN
VTATRCFDMY EGDNSGPMTK FIQSAAPKSL LFMVTYDDGS TRLNNDAKNA IEALGSKEIR
NMKFRSSWVF IAAKGLELPS EIQREKINHS DAKNNRYSGW PAEIQIEGCI PKERS
