FAM3B_MOUSE
ID FAM3B_MOUSE Reviewed; 235 AA.
AC Q9D309; O88417;
DT 19-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Protein FAM3B;
DE AltName: Full=Cytokine-like protein 2-21;
DE AltName: Full=Pancreatic-derived factor;
DE Short=PANDER;
DE Flags: Precursor;
GN Name=Fam3b; Synonyms=ORF9;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION.
RC STRAIN=C57BL/6J;
RX PubMed=12160727; DOI=10.1006/geno.2002.6816;
RA Zhu Y., Xu G., Patel A., McLaughlin M.M., Silverman C., Knecht K.A.,
RA Sweitzer S., Li X., McDonnell P., Mirabile R., Zimmerman D., Boyce R.,
RA Tierney L.A., Hu E., Livi G.P., Wolf B.A., Abdel-Meguid S.S., Rose G.D.,
RA Aurora R., Hensley P., Briggs M., Young P.R.;
RT "Cloning, expression, and initial characterization of a novel cytokine-like
RT gene family.";
RL Genomics 80:144-150(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Colon;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11707072; DOI=10.1006/geno.2001.6640;
RA Reymond A., Friedli M., Neergaard Henrichsen C., Chapot F., Deutsch S.,
RA Ucla C., Rossier C., Lyle R., Guipponi M., Antonarakis S.E.;
RT "From PREDs and open reading frames to cDNA isolation: revisiting the human
RT chromosome 21 transcription map.";
RL Genomics 78:46-54(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 162-205.
RX PubMed=9750193; DOI=10.1101/gr.8.9.940;
RA Cabin D.E., McKee-Johnson J.W., Matesic L.E., Wiltshire T., Rue E.E.,
RA Mjaatvedt A.E., Huo Y.K., Korenberg J.R., Reeves R.H.;
RT "Physical and comparative mapping of distal mouse chromosome 16. 5 p5.";
RL Genome Res. 8:940-950(1998).
RN [6]
RP INDUCTION.
RX PubMed=17962352; DOI=10.1210/en.2007-0106;
RA Wang O., Cai K., Pang S., Wang T., Qi D., Zhu Q., Ni Z., Le Y.;
RT "Mechanisms of glucose-induced expression of pancreatic-derived factor in
RT pancreatic beta-cells.";
RL Endocrinology 149:672-680(2008).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 46-235, PARTIAL PROTEIN SEQUENCE,
RP FUNCTION, GLYCOSYLATION, DISULFIDE BONDS, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=23333428; DOI=10.1016/j.str.2012.12.009;
RA Johansson P., Bernstrom J., Gorman T., Oster L., Backstrom S.,
RA Schweikart F., Xu B., Xue Y., Schiavone L.H.;
RT "FAM3B PANDER and FAM3C ILEI Represent a Distinct Class of Signaling
RT Molecules with a Non-Cytokine-like Fold.";
RL Structure 21:306-313(2013).
CC -!- FUNCTION: Induces apoptosis of alpha and beta cells in a dose- and
CC time-dependent manner. {ECO:0000269|PubMed:23333428}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Note=Present in insulin
CC secretory granules and likely cosecreted with insulin. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed at high levels in the pancreas and, to a
CC lesser extent, in small intestine and prostate. Also detected in
CC stomach, testis and fetal liver. In the pancreas, localized in the
CC islets of Langerhans; in the testis, found primarily in the round
CC spermatids; in the CNS, found in the Purkinje cell layer of the
CC cerebellum and in nerve cell bodies of numerous brainstem nuclei.
CC -!- INDUCTION: By glucose. {ECO:0000269|PubMed:17962352}.
CC -!- PTM: O-glycosylated. {ECO:0000269|PubMed:23333428}.
CC -!- MISCELLANEOUS: It is unclear whether the N-terminus residue of the
CC mature protein is Glu-30 or Ser-46.
CC -!- SIMILARITY: Belongs to the FAM3 family. {ECO:0000305}.
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DR EMBL; AF494380; AAM94281.1; -; mRNA.
DR EMBL; AK018571; BAB31283.1; -; mRNA.
DR EMBL; AF360358; AAL34461.1; -; mRNA.
DR EMBL; BC048949; AAH48949.1; -; mRNA.
DR EMBL; AF045953; AAC21456.1; -; Genomic_DNA.
DR CCDS; CCDS37416.1; -.
DR RefSeq; NP_065647.1; NM_020622.3.
DR PDB; 2YOP; X-ray; 2.30 A; A/B/C=46-235.
DR PDB; 2YOQ; X-ray; 2.35 A; A/B/C=30-235.
DR PDBsum; 2YOP; -.
DR PDBsum; 2YOQ; -.
DR AlphaFoldDB; Q9D309; -.
DR SMR; Q9D309; -.
DR STRING; 10090.ENSMUSP00000062006; -.
DR GlyGen; Q9D309; 1 site.
DR PhosphoSitePlus; Q9D309; -.
DR MaxQB; Q9D309; -.
DR PaxDb; Q9D309; -.
DR PeptideAtlas; Q9D309; -.
DR PRIDE; Q9D309; -.
DR ProteomicsDB; 275586; -.
DR Antibodypedia; 2683; 311 antibodies from 29 providers.
DR DNASU; 52793; -.
DR Ensembl; ENSMUST00000049721; ENSMUSP00000062006; ENSMUSG00000022938.
DR GeneID; 52793; -.
DR KEGG; mmu:52793; -.
DR UCSC; uc008adj.1; mouse.
DR CTD; 54097; -.
DR MGI; MGI:1270150; Fam3b.
DR VEuPathDB; HostDB:ENSMUSG00000022938; -.
DR eggNOG; ENOG502QW7Y; Eukaryota.
DR GeneTree; ENSGT00950000183004; -.
DR HOGENOM; CLU_099478_1_1_1; -.
DR InParanoid; Q9D309; -.
DR OMA; FASVCAW; -.
DR OrthoDB; 1295040at2759; -.
DR PhylomeDB; Q9D309; -.
DR TreeFam; TF353414; -.
DR BioGRID-ORCS; 52793; 2 hits in 70 CRISPR screens.
DR ChiTaRS; Fam3b; mouse.
DR PRO; PR:Q9D309; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9D309; protein.
DR Bgee; ENSMUSG00000022938; Expressed in mucosa of stomach and 56 other tissues.
DR ExpressionAtlas; Q9D309; baseline and differential.
DR Genevisible; Q9D309; MM.
DR GO; GO:0005576; C:extracellular region; NAS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005641; C:nuclear envelope lumen; ISO:MGI.
DR GO; GO:0005125; F:cytokine activity; NAS:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0042593; P:glucose homeostasis; IEA:InterPro.
DR GO; GO:0030073; P:insulin secretion; IDA:UniProtKB.
DR InterPro; IPR039220; FAM3.
DR InterPro; IPR039214; FAM3B.
DR InterPro; IPR039477; ILEI/PANDER_dom.
DR PANTHER; PTHR14592; PTHR14592; 1.
DR PANTHER; PTHR14592:SF2; PTHR14592:SF2; 1.
DR Pfam; PF15711; ILEI; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Apoptosis; Cytokine; Direct protein sequencing;
KW Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..235
FT /note="Protein FAM3B"
FT /id="PRO_0000008751"
FT CARBOHYD 208
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 63..91
FT /evidence="ECO:0000269|PubMed:23333428"
FT DISULFID 69..229
FT /evidence="ECO:0000269|PubMed:23333428"
FT CONFLICT 162
FT /note="K -> R (in Ref. 5; AAC21456)"
FT /evidence="ECO:0000305"
FT HELIX 62..64
FT /evidence="ECO:0007829|PDB:2YOP"
FT STRAND 73..79
FT /evidence="ECO:0007829|PDB:2YOP"
FT TURN 83..85
FT /evidence="ECO:0007829|PDB:2YOP"
FT STRAND 89..92
FT /evidence="ECO:0007829|PDB:2YOP"
FT STRAND 95..99
FT /evidence="ECO:0007829|PDB:2YOP"
FT TURN 100..103
FT /evidence="ECO:0007829|PDB:2YOP"
FT STRAND 107..115
FT /evidence="ECO:0007829|PDB:2YOP"
FT TURN 116..118
FT /evidence="ECO:0007829|PDB:2YOP"
FT STRAND 121..127
FT /evidence="ECO:0007829|PDB:2YOP"
FT STRAND 129..133
FT /evidence="ECO:0007829|PDB:2YOP"
FT HELIX 135..144
FT /evidence="ECO:0007829|PDB:2YOP"
FT STRAND 147..157
FT /evidence="ECO:0007829|PDB:2YOP"
FT HELIX 165..172
FT /evidence="ECO:0007829|PDB:2YOP"
FT TURN 173..175
FT /evidence="ECO:0007829|PDB:2YOP"
FT HELIX 179..181
FT /evidence="ECO:0007829|PDB:2YOQ"
FT STRAND 187..196
FT /evidence="ECO:0007829|PDB:2YOP"
FT STRAND 205..208
FT /evidence="ECO:0007829|PDB:2YOP"
FT TURN 212..214
FT /evidence="ECO:0007829|PDB:2YOP"
FT STRAND 225..231
FT /evidence="ECO:0007829|PDB:2YOP"
FT TURN 232..235
FT /evidence="ECO:0007829|PDB:2YOP"
SQ SEQUENCE 235 AA; 26152 MW; 2539E5ED009D9C3C CRC64;
MRPVATGIFK ALVFIFSSLC AWYSGYLLAE LIPDVPLSST LYNIRSIGER PVLKAPAPKR
QKCDHWSPCP PDTYAYRLLS GGGRDKYAKI CFEDEVLIGE KTGNVARGIN IAVVNYETGK
VIATKYFDMY EGDNSGPMAK FIQSTPSKSL LFMVTHDDGS SKLKAQAKDA IEALGSKEIK
NMKFRSSWVF VAAKGFELPS EIEREKINHS DQSRNRYAGW PAEIQIEGCI PKGLR
