FAO1_ARATH
ID FAO1_ARATH Reviewed; 758 AA.
AC Q9ZWB9; F4I439;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Long-chain-alcohol oxidase FAO1;
DE EC=1.1.3.20;
DE AltName: Full=Long-chain fatty alcohol oxidase 1;
GN Name=FAO1; OrderedLocusNames=At1g03990; ORFNames=F21M11.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Long-chain fatty alcohol oxidase involved in the omega-
CC oxidation pathway of lipid degradation. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a long-chain primary fatty alcohol + O2 = a long-chain fatty
CC aldehyde + H2O2; Xref=Rhea:RHEA:22756, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:17176, ChEBI:CHEBI:77396; EC=1.1.3.20;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD10696.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC003027; AAD10696.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27643.1; -; Genomic_DNA.
DR PIR; A86171; A86171.
DR RefSeq; NP_171895.2; NM_100280.3.
DR AlphaFoldDB; Q9ZWB9; -.
DR SMR; Q9ZWB9; -.
DR STRING; 3702.AT1G03990.1; -.
DR PaxDb; Q9ZWB9; -.
DR PRIDE; Q9ZWB9; -.
DR ProteomicsDB; 230849; -.
DR EnsemblPlants; AT1G03990.1; AT1G03990.1; AT1G03990.
DR GeneID; 839342; -.
DR Gramene; AT1G03990.1; AT1G03990.1; AT1G03990.
DR KEGG; ath:AT1G03990; -.
DR Araport; AT1G03990; -.
DR TAIR; locus:2024157; AT1G03990.
DR eggNOG; ENOG502QSD8; Eukaryota.
DR HOGENOM; CLU_008878_1_1_1; -.
DR InParanoid; Q9ZWB9; -.
DR OMA; SMSKHWT; -.
DR OrthoDB; 626762at2759; -.
DR BioCyc; ARA:AT1G03990-MON; -.
DR PRO; PR:Q9ZWB9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9ZWB9; baseline and differential.
DR Genevisible; Q9ZWB9; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0046577; F:long-chain-alcohol oxidase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000172; GMC_OxRdtase_N.
DR InterPro; IPR007867; GMC_OxRtase_C.
DR InterPro; IPR012400; Long_Oxdase.
DR Pfam; PF05199; GMC_oxred_C; 1.
DR Pfam; PF00732; GMC_oxred_N; 1.
DR PIRSF; PIRSF028937; Lg_Ch_AO; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 3: Inferred from homology;
KW FAD; Flavoprotein; Membrane; Oxidoreductase; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..758
FT /note="Long-chain-alcohol oxidase FAO1"
FT /id="PRO_0000395503"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 689
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:E4QP00"
FT BINDING 246..261
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
SQ SEQUENCE 758 AA; 83864 MW; BD40A3AEF04695F5 CRC64;
MVGGRRLGKR GSPLLRWSVK QESFSHGFSK SDLQALSSIC DAIMPPVPLE SLNLEMKLKV
LRNDALLSFF KSSSSESHVR PDEVAELLAT KAIPLTVLVV RIVLRILTFR LGTLLLCGLV
CLDKKHWPFL LKFSEMSLEK REKVLQRWNT QWYNPLARIG FMMIKAIFLF YYFTWTNENS
ENPVWDAINY SVEIGENEDM EQKERPLDEG IIETAKEDEM TIKQRMINKG LKVTEDRERD
TYKIECDAVV VGSGCGGGVA AAILAKSGLR VVVIEKGNYF APRDYSALEG PSMFELFESN
SLMMTHDGRF RFMAGSTVGG GSVVNWAASL KTPDAIIEEW SVHRGISIYS SEKYKAAMGI
VCKRLGVTEK IIREGFQNQI LRKGCEKLGL DVTIVPRNST EKHYCGSCSY GCPTGEKRGT
DSTWLVDAVN NNAVILTQCK AEKLILADND ANKREESGRR KRCLGVAASL SHQTRKKLQI
NAKVTIVACG SLKTPGLLAS SGLKNSNISR GLHIHPIMMA WGYFPEKNSE LEGAAHEGEI
VTSLHYVHPM DSTTPNITLE TPAIGPGTFA ALTPWVSGSD MKERMAKYAR TAHIFAMVRD
EGVGEVKGDI VKYRLTKADE ENLTIGLKQA LRILVAAGAA EVGTYRSDGQ RMKCDGIKQK
DLEAFLDTVN APPGVVSMSK HWTQSFTAHQ IGCCRMGATE KEGAIDGKGE SWEAEDLYVC
DASVLPTALG VNPMITVQST AYCISNRIAE LMKKRKKD
