FAPR_BACSU
ID FAPR_BACSU Reviewed; 188 AA.
AC O34835;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Transcription factor FapR;
DE AltName: Full=Fatty acid and phospholipid biosynthesis regulator;
GN Name=fapR; Synonyms=ylpC; OrderedLocusNames=BSU15880;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9534248; DOI=10.1099/00221287-144-3-801;
RA Foulger D., Errington J.;
RT "A 28 kbp segment from the spoVM region of the Bacillus subtilis 168
RT genome.";
RL Microbiology 144:801-805(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP PROTEIN SEQUENCE OF 1-9, FUNCTION, DNA-BINDING, AND INDUCTION.
RX PubMed=12737802; DOI=10.1016/s1534-5807(03)00123-0;
RA Schujman G.E., Paoletti L., Grossman A.D., de Mendoza D.;
RT "FapR, a bacterial transcription factor involved in global regulation of
RT membrane lipid biosynthesis.";
RL Dev. Cell 4:663-672(2003).
CC -!- FUNCTION: Transcription factor involved in regulation of membrane lipid
CC biosynthesis by repressing genes involved in fatty acid and
CC phospholipid metabolism. Binds to the 5'-TTAGTANNNNNTANTAA-3' consensus
CC sequence found in the promoter of fabHAF operon (containing fabHA and
CC fabF genes), yhdO and fapR genes and prevents their expression. Its
CC action is probably modulated by malonyl-CoA.
CC {ECO:0000269|PubMed:12737802}.
CC -!- INDUCTION: Autoregulated. {ECO:0000269|PubMed:12737802}.
CC -!- SIMILARITY: Belongs to the FapR family. {ECO:0000305}.
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DR EMBL; Y13937; CAA74247.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13461.1; -; Genomic_DNA.
DR PIR; A69880; A69880.
DR RefSeq; NP_389470.1; NC_000964.3.
DR RefSeq; WP_003232044.1; NZ_JNCM01000035.1.
DR PDB; 2F3X; X-ray; 3.10 A; A/B=44-188.
DR PDB; 2F41; X-ray; 2.50 A; A/B/C/D=68-188.
DR PDBsum; 2F3X; -.
DR PDBsum; 2F41; -.
DR AlphaFoldDB; O34835; -.
DR SMR; O34835; -.
DR IntAct; O34835; 6.
DR STRING; 224308.BSU15880; -.
DR PaxDb; O34835; -.
DR PRIDE; O34835; -.
DR EnsemblBacteria; CAB13461; CAB13461; BSU_15880.
DR GeneID; 936595; -.
DR KEGG; bsu:BSU15880; -.
DR PATRIC; fig|224308.179.peg.1728; -.
DR eggNOG; COG1349; Bacteria.
DR eggNOG; COG2050; Bacteria.
DR OMA; GIARGHH; -.
DR PhylomeDB; O34835; -.
DR BioCyc; BSUB:BSU15880-MON; -.
DR EvolutionaryTrace; O34835; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0045717; P:negative regulation of fatty acid biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_01814; Transcrip_fact_FapR; 1.
DR InterPro; IPR029069; HotDog_dom_sf.
DR InterPro; IPR006683; Thioestr_dom.
DR InterPro; IPR017275; Transcription_factor_FapR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF03061; 4HBT; 1.
DR PIRSF; PIRSF037733; Transcription_factor_FapR; 1.
DR SUPFAM; SSF54637; SSF54637; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA-binding;
KW Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis;
KW Lipid metabolism; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..188
FT /note="Transcription factor FapR"
FT /id="PRO_0000172819"
FT HELIX 47..63
FT /evidence="ECO:0007829|PDB:2F3X"
FT HELIX 71..73
FT /evidence="ECO:0007829|PDB:2F3X"
FT STRAND 75..82
FT /evidence="ECO:0007829|PDB:2F41"
FT TURN 83..85
FT /evidence="ECO:0007829|PDB:2F41"
FT STRAND 86..92
FT /evidence="ECO:0007829|PDB:2F41"
FT HELIX 95..97
FT /evidence="ECO:0007829|PDB:2F41"
FT TURN 100..102
FT /evidence="ECO:0007829|PDB:2F3X"
FT HELIX 107..120
FT /evidence="ECO:0007829|PDB:2F41"
FT STRAND 129..136
FT /evidence="ECO:0007829|PDB:2F41"
FT STRAND 145..155
FT /evidence="ECO:0007829|PDB:2F41"
FT STRAND 157..160
FT /evidence="ECO:0007829|PDB:2F41"
FT STRAND 162..170
FT /evidence="ECO:0007829|PDB:2F41"
FT STRAND 173..182
FT /evidence="ECO:0007829|PDB:2F41"
SQ SEQUENCE 188 AA; 21400 MW; CF38F3781C20BD63 CRC64;
MRRNKRERQE LLQQTIQATP FITDEELAGK FGVSIQTIRL DRLELSIPEL RERIKNVAEK
TLEDEVKSLS LDEVIGEIID LELDDQAISI LEIKQEHVFS RNQIARGHHL FAQANSLAVA
VIDDELALTA SADIRFTRQV KQGERVVAKA KVTAVEKEKG RTVVEVNSYV GEEIVFSGRF
DMYRSKHS
