FAR1_YEAST
ID FAR1_YEAST Reviewed; 830 AA.
AC P21268; D6VW30;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 184.
DE RecName: Full=Cyclin-dependent kinase inhibitor FAR1;
DE Short=CKI FAR1;
DE AltName: Full=Factor arrest protein;
GN Name=FAR1; OrderedLocusNames=YJL157C; ORFNames=J0565;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2147873; DOI=10.1016/0092-8674(90)90503-7;
RA Chang F., Herskowitz I.;
RT "Identification of a gene necessary for cell cycle arrest by a negative
RT growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2.";
RL Cell 63:999-1011(1990).
RN [2]
RP SEQUENCE REVISION TO N-TERMINUS, AND DISRUPTION PHENOTYPE.
RX PubMed=7739534; DOI=10.1128/mcb.15.5.2509;
RA McKinney J.D., Cross F.R.;
RT "FAR1 and the G1 phase specificity of cell cycle arrest by mating factor in
RT Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 15:2509-2516(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP REVIEW.
RX PubMed=15335864; DOI=10.1016/0960-9822(93)90071-u;
RA Chang F.;
RT "Stop that cell cycle.";
RL Curr. Biol. 3:693-695(1993).
RN [6]
RP CHARACTERIZATION.
RX PubMed=8066461; DOI=10.1126/science.8066461;
RA Peter M., Herskowitz I.;
RT "Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by
RT Far1.";
RL Science 265:1228-1231(1994).
RN [7]
RP CHARACTERIZATION.
RX PubMed=7490290; DOI=10.1083/jcb.131.4.863;
RA Valtz N., Peter M., Herskowitz I.;
RT "FAR1 is required for oriented polarization of yeast cells in response to
RT mating pheromones.";
RL J. Cell Biol. 131:863-873(1995).
RN [8]
RP CHARACTERIZATION.
RX PubMed=9251033; DOI=10.1016/s0076-6879(97)83029-7;
RA Valtz N., Peter M.;
RT "Functional analysis of FAR1 in yeast.";
RL Methods Enzymol. 283:350-365(1997).
RN [9]
RP CHARACTERIZATION.
RX PubMed=9367986; DOI=10.1101/gad.11.22.3046;
RA Henchoz S., Chi Y., Catarin B., Herskowitz I., Deshaies R.J., Peter M.;
RT "Phosphorylation- and ubiquitin-dependent degradation of the cyclin-
RT dependent kinase inhibitor Far1p in budding yeast.";
RL Genes Dev. 11:3046-3060(1997).
RN [10]
RP MUTAGENESIS OF SER-87 AND THR-306.
RX PubMed=9632750; DOI=10.1128/mcb.18.7.3681;
RA Gartner A., Jovanovic A., Jeoung D.I., Bourlat S., Cross F.R., Ammerer G.;
RT "Pheromone-dependent G1 cell cycle arrest requires Far1 phosphorylation,
RT but may not involve inhibition of Cdc28-Cln2 kinase, in vivo.";
RL Mol. Cell. Biol. 18:3681-3691(1998).
RN [11]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; SER-114 AND THR-306, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
CC -!- FUNCTION: Inhibitor of the cyclin-dependent kinase CDC28. Necessary for
CC cell cycle arrest. Involved in pheromone response. Contributes to
CC mating efficiency. Required for oriented polarization of yeast cells in
CC response to mating pheromones.
CC -!- SUBUNIT: Associates with the CDC28-CLN complex.
CC -!- INTERACTION:
CC P21268; P11433: CDC24; NbExp=8; IntAct=EBI-6780, EBI-4220;
CC P21268; P18851: STE4; NbExp=2; IntAct=EBI-6780, EBI-7390;
CC -!- INDUCTION: By alpha-factor in a cells.
CC -!- DOMAIN: There is evidence to suggest that the N-terminal part may be
CC sufficient for cell cycle arrest and the C-terminal may be necessary
CC for some step in mating.
CC -!- PTM: Thought to be phosphorylated by MAP kinase FUS3. Thought to
CC enhance the binding of FAR1 to G1-specific cyclin-dependent kinase
CC (CDK) complexes.
CC -!- DISRUPTION PHENOTYPE: Some mutants appear to be defective in mating
CC because they are unable to locate the mating partner.
CC {ECO:0000269|PubMed:7739534}.
CC -!- MISCELLANEOUS: Present with 238 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA34600.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M60071; AAA34600.1; ALT_INIT; Genomic_DNA.
DR EMBL; Z49432; CAA89452.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08646.1; -; Genomic_DNA.
DR PIR; S56940; S56940.
DR RefSeq; NP_012378.1; NM_001181590.1.
DR AlphaFoldDB; P21268; -.
DR BioGRID; 33603; 89.
DR ComplexPortal; CPX-977; CDC24-FAR1-Gbetagamma complex.
DR DIP; DIP-2229N; -.
DR IntAct; P21268; 37.
DR MINT; P21268; -.
DR STRING; 4932.YJL157C; -.
DR iPTMnet; P21268; -.
DR MaxQB; P21268; -.
DR PaxDb; P21268; -.
DR PRIDE; P21268; -.
DR EnsemblFungi; YJL157C_mRNA; YJL157C; YJL157C.
DR GeneID; 853283; -.
DR KEGG; sce:YJL157C; -.
DR SGD; S000003693; FAR1.
DR VEuPathDB; FungiDB:YJL157C; -.
DR eggNOG; ENOG502QSDX; Eukaryota.
DR HOGENOM; CLU_341046_0_0_1; -.
DR InParanoid; P21268; -.
DR OMA; FIGMINK; -.
DR BioCyc; YEAST:G3O-31597-MON; -.
DR PRO; PR:P21268; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P21268; protein.
DR GO; GO:0120171; C:Cdc24p-Far1p-Gbetagamma complex; IDA:SGD.
DR GO; GO:0005938; C:cell cortex; IC:ComplexPortal.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0043332; C:mating projection tip; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; IDA:SGD.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0043577; P:chemotropism; IMP:SGD.
DR GO; GO:0051457; P:maintenance of protein location in nucleus; IMP:SGD.
DR GO; GO:0000751; P:mitotic cell cycle G1 arrest in response to pheromone; IMP:SGD.
DR GO; GO:0010969; P:regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion; IC:ComplexPortal.
DR InterPro; IPR001841; Znf_RING.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Metal-binding; Phosphoprotein;
KW Protein kinase inhibitor; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..830
FT /note="Cyclin-dependent kinase inhibitor FAR1"
FT /id="PRO_0000055910"
FT ZN_FING 202..252
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 87
FT /note="Phosphoserine; by CDC28"
FT /evidence="ECO:0000305"
FT MOD_RES 110
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 114
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 306
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT VARIANT 87
FT /note="S -> P (in FAR1-22P; induces cell cycle arrest in
FT the absence of alpha-factor)"
FT MUTAGEN 87
FT /note="S->A: Prevents cell cycle-dependent degradation of
FT FAR1."
FT /evidence="ECO:0000269|PubMed:9632750"
FT MUTAGEN 306
FT /note="T->A: Abolishes G1 arrest function."
FT /evidence="ECO:0000269|PubMed:9632750"
FT CONFLICT 20
FT /note="D -> N (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 568
FT /note="P -> R (in Ref. 1; AAA34600)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 830 AA; 94572 MW; 834EBB2D7BB9641D CRC64;
MKTPTRVSFE KKIHTPPSGD RDAERSPPKK FLRGLSGKVF RKTPEFKKQQ MPTFGYIEES
QFTPNLGLMM SKRGNIPKPL NLSKPISPPP SLKKTAGSVA SGFSKTGQLS ALQSPVNITS
SNKYNIKATN LTTSLLRESI SDSTTMCDTL SDINLTVMDE DYRIDGDSYY EEDSPTFMIS
LERNIKKCNS QFSPKRYIGE KCLICEESIS STFTGEKVVE STCSHTSHYN CYLMLFETLY
FQGKFPECKI CGEVSKPKDK DIVPEMVSKL LTGAGAHDDG PSSNMQQQWI DLKTARSFTG
EFPQFTPQEQ LIRTADISCD GFRTPRLSNS NQFEAVSYLD SPFLNSPFVN KMATTDPFDL
SDDEKLDCDD EIDESAAEVW FSKTGGEHVM VSVKFQEMRT SDDLGVLQDV NHVDHEELEE
REKEWKKKID QYIETNVDKD SEFGSLILFD KLMYSDDGEQ WVDNNLVILF SKFLVLFDFE
EMKILGKIPR DQFYQVIKFN EDVLLCSLKS TNIPEIYLRF NENCEKWLLP KWKYCLENSS
LETLPLSEIV STVKELSHVN IIGALGAPPD VISAQSHDSR LPWKRLHSDT PLKLIVCLNL
SHADGELYRK RVLKSVHQIL DGLNTDDLLG IVVVGRDGSG VVGPFGTFIG MINKNWDGWT
TFLDNLEVVN PNVFRDEKQQ YKVTLQTCER LASTSAYVDT DDHIATGYAK QILVLNGSDV
VDIEHDQKLK KAFDQLSYHW RYEISQRRMT PLNASIKQFL EELHTKRYLD VTLRLPQATF
EQVYLGDMAA GEQKTRLIMD EHPHSSLIEI EYFDLVKQQR IHQTLEVPNL
