FAR8_YEAST
ID FAR8_YEAST Reviewed; 523 AA.
AC Q05040; D6VZK3;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Factor arrest protein 8;
GN Name=FAR8; OrderedLocusNames=YMR029C; ORFNames=YM9973.02C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, AND INTERACTION WITH FAR3; FAR7; FAR10; FAR11 AND VPS64.
RX PubMed=12588993; DOI=10.1128/mcb.23.5.1750-1763.2003;
RA Kemp H.A., Sprague G.F. Jr.;
RT "Far3 and five interacting proteins prevent premature recovery from
RT pheromone arrest in the budding yeast Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 23:1750-1763(2003).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-132, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-132, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Participates in the control of the reentry into the cell
CC cycle following pheromone treatment. {ECO:0000269|PubMed:12588993}.
CC -!- SUBUNIT: Component of a complex at least composed of FAR3, FAR7, FAR8,
CC FAR10, FAR11 and VPS64.
CC -!- INTERACTION:
CC Q05040; P46671: FAR3; NbExp=3; IntAct=EBI-28053, EBI-6789;
CC Q05040; P02994: TEF2; NbExp=2; IntAct=EBI-28053, EBI-6314;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC Endoplasmic reticulum {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 4010 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z49213; CAA89144.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09927.1; -; Genomic_DNA.
DR PIR; S53945; S53945.
DR RefSeq; NP_013742.1; NM_001182525.1.
DR AlphaFoldDB; Q05040; -.
DR SMR; Q05040; -.
DR BioGRID; 35201; 125.
DR ComplexPortal; CPX-1197; FAR complex.
DR DIP; DIP-6343N; -.
DR IntAct; Q05040; 10.
DR MINT; Q05040; -.
DR STRING; 4932.YMR029C; -.
DR iPTMnet; Q05040; -.
DR MaxQB; Q05040; -.
DR PaxDb; Q05040; -.
DR PRIDE; Q05040; -.
DR EnsemblFungi; YMR029C_mRNA; YMR029C; YMR029C.
DR GeneID; 855044; -.
DR KEGG; sce:YMR029C; -.
DR SGD; S000004631; FAR8.
DR VEuPathDB; FungiDB:YMR029C; -.
DR eggNOG; KOG0642; Eukaryota.
DR GeneTree; ENSGT00950000183095; -.
DR HOGENOM; CLU_034775_0_0_1; -.
DR InParanoid; Q05040; -.
DR OMA; YLQTEFT; -.
DR BioCyc; YEAST:G3O-32734-MON; -.
DR PRO; PR:Q05040; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q05040; protein.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IC:ComplexPortal.
DR GO; GO:0090443; C:FAR/SIN/STRIPAK complex; IC:ComplexPortal.
DR GO; GO:0071444; P:cellular response to pheromone; IC:ComplexPortal.
DR GO; GO:0000321; P:re-entry into mitotic cell cycle after pheromone arrest; IGI:SGD.
DR GO; GO:0051726; P:regulation of cell cycle; IC:ComplexPortal.
DR InterPro; IPR013258; Striatin_N.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF08232; Striatin; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Coiled coil; Cytoplasm; Endoplasmic reticulum; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..523
FT /note="Factor arrest protein 8"
FT /id="PRO_0000087193"
FT REGION 61..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 150..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 26..76
FT /evidence="ECO:0000255"
FT MOD_RES 115
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 132
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:19779198"
SQ SEQUENCE 523 AA; 59301 MW; 3B2A3C95B2D575AE CRC64;
MAINQAHVHP HYTLPGVMHY LQTEFTKNER DRITWELERS EMKARIAELE GENRDLKHQL
NQIQSKAVSP EGEKEEKHVP DSLLQSKLAV QENVKEIIYL LKGPNTVNQL ESLNSREAGS
ELHDLEKLNV NTPKEEGSAK TNGMDILNNA LLDTKPNPKQ GPSESPSPTK VKSLFSTANK
RENNETISKI HSELSKVDII SSYGDCMALY DADTKSLEIH QVDANLNSKL LKKISLGQDS
DIMKFFWVST SKLLVIEKSF HLKLFSISSA SLISDVDLLQ DSEQPFSSSD IINIDFKNKW
LLIASKNKSQ IRIWELDNIE APEDVPINIK ETYEITHDND DDDSNDSTNI LDCILGITEK
SLILLSSNPY QLTIYDFEGK LLQKIDLKID TILSGKPEEE GYHLFLDRKT SKLLIQLSNE
RLLVYSFDKK KVVLKEQLTP SSTLPIQLDL NDSIITVSYS NGDFEFRNLE NLKPSIDEFV
VADINFSERK EPVVFSSNLI VDSTPVLITV NKNNEVLLHK IKI
