FARNS_PICXS
ID FARNS_PICXS Reviewed; 580 AA.
AC F2XF99;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 39.
DE RecName: Full=(E,E)-alpha-farnesene synthase {ECO:0000303|PubMed:21385377};
DE EC=4.2.3.46 {ECO:0000269|PubMed:21385377};
DE AltName: Full=(E)-beta-ocimene synthase {ECO:0000303|PubMed:21385377};
DE EC=4.2.3.106 {ECO:0000269|PubMed:21385377};
DE AltName: Full=Terpene synthase TPS-Far/Oci {ECO:0000303|PubMed:21385377};
DE Short=PgxeTPS-Far/Oci {ECO:0000303|PubMed:21385377};
GN Name=TPS-Far/Oci {ECO:0000303|PubMed:21385377};
OS Picea engelmannii x Picea glauca (Hybrid white spruce).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX NCBI_TaxID=373101;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION, PATHWAY, AND GENE
RP FAMILY.
RC STRAIN=cv. Fa1-1028;
RX PubMed=21385377; DOI=10.1186/1471-2229-11-43;
RA Keeling C.I., Weisshaar S., Ralph S.G., Jancsik S., Hamberger B.,
RA Dullat H.K., Bohlmann J.;
RT "Transcriptome mining, functional characterization, and phylogeny of a
RT large terpene synthase gene family in spruce (Picea spp.).";
RL BMC Plant Biol. 11:43-43(2011).
CC -!- FUNCTION: Terpene synthase (TPS) involved in the biosynthesis of
CC sesquiterpene and monoterpene natural products included in conifer
CC oleoresin secretions and volatile emissions; these compounds contribute
CC to biotic and abiotic stress defense against herbivores and pathogens
CC (PubMed:21385377). Catalyzes the conversion of (2E,6E)-farnesyl
CC diphosphate (FPP) to (3E,6E)-alpha-farnesene and of (2E)-geranyl
CC diphosphate (GPP) to (E)-beta-ocimene (PubMed:21385377).
CC {ECO:0000269|PubMed:21385377}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = (3E,6E)-alpha-farnesene +
CC diphosphate; Xref=Rhea:RHEA:27421, ChEBI:CHEBI:10280,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:175763; EC=4.2.3.46;
CC Evidence={ECO:0000269|PubMed:21385377};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = (E)-beta-ocimene + diphosphate;
CC Xref=Rhea:RHEA:32691, ChEBI:CHEBI:33019, ChEBI:CHEBI:58057,
CC ChEBI:CHEBI:64280; EC=4.2.3.106;
CC Evidence={ECO:0000269|PubMed:21385377};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:A0A1C9J6A7};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:A0A1C9J6A7};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit.
CC {ECO:0000250|UniProtKB:A0A1C9J6A7};
CC -!- PATHWAY: Terpene metabolism; oleoresin biosynthesis.
CC {ECO:0000269|PubMed:21385377}.
CC -!- PATHWAY: Sesquiterpene biosynthesis. {ECO:0000269|PubMed:21385377}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250|UniProtKB:A0A1C9J6A7}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsd subfamily.
CC {ECO:0000305}.
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DR EMBL; HQ426157; ADZ45514.1; -; mRNA.
DR UniPathway; UPA00924; -.
DR GO; GO:0034768; F:(E)-beta-ocimene synthase activity; IDA:UniProtKB.
DR GO; GO:0052578; F:alpha-farnesene synthase activity; IDA:UniProtKB.
DR GO; GO:0016829; F:lyase activity; IDA:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:0010597; P:green leaf volatile biosynthetic process; IDA:UniProtKB.
DR GO; GO:0016099; P:monoterpenoid biosynthetic process; IDA:UniProtKB.
DR GO; GO:0016106; P:sesquiterpenoid biosynthetic process; IDA:UniProtKB.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Lyase; Magnesium; Metal-binding.
FT CHAIN 1..580
FT /note="(E,E)-alpha-farnesene synthase"
FT /id="PRO_0000454400"
FT MOTIF 331..335
FT /note="DDXXD motif"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 331
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 331
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 335
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 335
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 483
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
SQ SEQUENCE 580 AA; 66198 MW; CA1FC9292AE8CEAC CRC64;
MASVDQSQLC SKSVSMSLSV DDGVQRRTGD YHSNLWDDDF IQSLSTPYGA PCYRERAERL
IGEVKEMFNS VRLSPLNDLL QGLSMVDSVE RLGIDRHFKN EIKSALDYVY SYWSEKGIGC
GRESVVTDLN SSALGFRALR LHGYPVSSDV FKDQNGQFAC SANTQTEGEM RGVLNLFRAS
LVAFPGEKVM EDAERFSAIY LKEALKTVPI CSGSLSGEIE YVLEYGWLTN FPRLEARNYI
DIFGKDTSPC LQTEKLLELA KLEFNIFHSL QQRELKQVSR WWKDSGFSQL TFTRHRHVEF
YTLASCIAIE PKHSAFRLGF AKLCYLGIVL DDIYDTFGTM DELELFTAAI KRWDPSATEC
LPEYMKGVYM VFYECVNQMA REAEKTQGRD TLSYARNTWE AVFDAFLEEA KWISSGYIPT
FEEYLENGKV SFGYRAATLQ PILTLDVPLP LHILQEIDFP SRFNDLAASI LRLRGDVCGY
KAERSRGEEA SSISCYMKDN PGATEEDALN QIDTMIKEKI KELNWEFLKP DSNVPISSKK
HAFDILRAFY HLYKYRDGFS IANNETKSLV MRTLLETVPF
