ID   FARR_NEIGO              Reviewed;         146 AA.
AC   P0DPR8;
DT   16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2019, sequence version 1.
DT   25-MAY-2022, entry version 14.
DE   RecName: Full=HTH-type transcriptional regulator FarR {ECO:0000305};
GN   Name=farR {ECO:0000303|PubMed:14645274};
GN   Synonyms=marR1 {ECO:0000303|PubMed:14645274};
GN   ORFNames=VT05_02083 {ECO:0000312|EMBL:AKP11729.1};
OS   Neisseria gonorrhoeae.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=485;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FA19;
RX   PubMed=26358608; DOI=10.1128/genomea.01052-15;
RA   Abrams A.J., Trees D.L., Nicholas R.A.;
RT   "Complete genome sequences of three Neisseria gonorrhoeae laboratory
RT   reference strains, determined using PacBio single-molecule real-time
RT   technology.";
RL   Genome Announc. 3:E01052-E01052(2015).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-9, FUNCTION, DNA-BINDING, AND INDUCTION.
RC   STRAIN=FA19;
RX   PubMed=14645274; DOI=10.1128/jb.185.24.7145-7152.2003;
RA   Lee E.H., Rouquette-Loughlin C., Folster J.P., Shafer W.M.;
RT   "FarR regulates the farAB-encoded efflux pump of Neisseria gonorrhoeae via
RT   an MtrR regulatory mechanism.";
RL   J. Bacteriol. 185:7145-7152(2003).
RN   [3]
RP   FUNCTION, DNA-BINDING, AND ACTIVITY REGULATION.
RC   STRAIN=FA19;
RX   PubMed=16796676; DOI=10.1111/j.1365-2958.2006.05185.x;
RA   Lee E.H., Hill S.A., Napier R., Shafer W.M.;
RT   "Integration Host Factor is required for FarR repression of the farAB-
RT   encoded efflux pump of Neisseria gonorrhoeae.";
RL   Mol. Microbiol. 60:1381-1400(2006).
CC   -!- FUNCTION: Negatively controls expression of the farAB operon by binding
CC       directly to the farAB promoter region (PubMed:14645274,
CC       PubMed:16796676). Binds to three sites (sites A, B and C) within the
CC       DNA sequence upstream of farA (PubMed:16796676). Also represses its own
CC       expression (PubMed:14645274). {ECO:0000269|PubMed:14645274,
CC       ECO:0000269|PubMed:16796676}.
CC   -!- ACTIVITY REGULATION: Repressor activity requires the presence of the
CC       Integration Host Factor (IHF), which binds to sequences located between
CC       FarR binding sites A and C. IHF binding to the promoter region
CC       stabilizes the binding of FarR to its binding sites A and C and as a
CC       consequence, enhances repression of the farAB operon.
CC       {ECO:0000269|PubMed:16796676}.
CC   -!- INDUCTION: Repressed by the HTH-type transcriptional regulator MtrR.
CC       Negatively autoregulated. {ECO:0000269|PubMed:14645274}.
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DR   EMBL; CP012026; AKP11729.1; -; Genomic_DNA.
DR   RefSeq; WP_003687296.1; NZ_WHPL01000002.1.
DR   AlphaFoldDB; P0DPR8; -.
DR   SMR; P0DPR8; -.
DR   GeneID; 66752321; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR012712; HpaR/FarR.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR023187; Tscrpt_reg_MarR-type_CS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01047; MarR; 1.
DR   PRINTS; PR00598; HTHMARR.
DR   SMART; SM00347; HTH_MARR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR02337; HpaR; 1.
DR   PROSITE; PS01117; HTH_MARR_1; 1.
DR   PROSITE; PS50995; HTH_MARR_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA-binding; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..146
FT                   /note="HTH-type transcriptional regulator FarR"
FT                   /id="PRO_0000445985"
FT   DOMAIN          7..139
FT                   /note="HTH marR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
FT   DNA_BIND        53..76
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
SQ   SEQUENCE   146 AA;  16615 MW;  A4548DDAA8791D47 CRC64;
     MPTQSKHASI NIGLIQAREA LMTQFRPILN QANITDQQWR IIRLLAENGT LDFQDLANQA
     CILRPSLTGI LTRLEKAGLV VRLKPSNDQR RVYLKLTSEG EKLYEEIGEE VDERYDAIEE
     VLGREKMLLL KDLLAELAKI EDALNS