FAS2_ARATH
ID FAS2_ARATH Reviewed; 487 AA.
AC Q9SXY1; Q94JQ9;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Chromatin assembly factor 1 subunit FAS2;
DE Short=CAF-1 subunit FAS2;
DE AltName: Full=CAF-1 p60 homolog;
DE AltName: Full=Protein FASCIATA 2;
GN Name=FAS2; Synonyms=NFB1; OrderedLocusNames=At5g64630; ORFNames=MUB3.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=11163246; DOI=10.1016/s0092-8674(01)00197-0;
RA Kaya H., Shibahara K., Taoka K., Iwabuchi M., Stillman B., Araki T.;
RT "FASCIATA genes for chromatin assembly factor-1 in Arabidopsis maintain the
RT cellular organization of apical meristems.";
RL Cell 104:131-142(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION.
RX PubMed=17021044; DOI=10.1242/dev.02599;
RA Exner V., Taranto P., Schoenrock N., Gruissem W., Hennig L.;
RT "Chromatin assembly factor CAF-1 is required for cellular differentiation
RT during plant development.";
RL Development 133:4163-4172(2006).
RN [6]
RP FUNCTION.
RX PubMed=17110925; DOI=10.1038/sj.emboj.7601434;
RA Endo M., Ishikawa Y., Osakabe K., Nakayama S., Kaya H., Araki T.,
RA Shibahara K., Abe K., Ichikawa H., Valentine L., Hohn B., Toki S.;
RT "Increased frequency of homologous recombination and T-DNA integration in
RT Arabidopsis CAF-1 mutants.";
RL EMBO J. 25:5579-5590(2006).
RN [7]
RP FUNCTION.
RX PubMed=16452472; DOI=10.1074/jbc.m513426200;
RA Schoenrock N., Exner V., Probst A., Gruissem W., Hennig L.;
RT "Functional genomic analysis of CAF-1 mutants in Arabidopsis thaliana.";
RL J. Biol. Chem. 281:9560-9568(2006).
RN [8]
RP FUNCTION.
RX PubMed=18045841; DOI=10.1242/dev.010108;
RA Chen Z., Tan J.L., Ingouff M., Sundaresan V., Berger F.;
RT "Chromatin assembly factor 1 regulates the cell cycle but not cell fate
RT during male gametogenesis in Arabidopsis thaliana.";
RL Development 135:65-73(2008).
RN [9]
RP FUNCTION.
RX PubMed=20699390; DOI=10.1105/tpc.110.076182;
RA Mozgova I., Mokros P., Fajkus J.;
RT "Dysfunction of chromatin assembly factor 1 induces shortening of telomeres
RT and loss of 45S rDNA in Arabidopsis thaliana.";
RL Plant Cell 22:2768-2780(2010).
CC -!- FUNCTION: Component of the chromatin assembly factor complex (CAF-1)
CC involved in chromatin assembly following DNA replication and DNA
CC repair. Required for several aspects of development, including seedling
CC growth and leaf hair differentiation. Plays a critical role in the
CC organization of shoot apical meristem (SAM) and root apical meristem
CC (RAM) during postembryonic development by facilitating stable
CC maintenance of gene expression states. Seems not required to maintain
CC transcriptional repression of heterochromatic genes. Involved in
CC heterologous recombination. {ECO:0000269|PubMed:11163246,
CC ECO:0000269|PubMed:16452472, ECO:0000269|PubMed:17021044,
CC ECO:0000269|PubMed:17110925, ECO:0000269|PubMed:18045841,
CC ECO:0000269|PubMed:20699390}.
CC -!- SUBUNIT: Component of the chromatin assembly factor 1 (CAF-1) complex,
CC composed of FAS1, FAS2 and MSI1. {ECO:0000269|PubMed:11163246}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SXY1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SXY1-2; Sequence=VSP_044413, VSP_044414;
CC -!- DISRUPTION PHENOTYPE: Fasciated plants with broad, flat stems and
CC disrupted phyllotaxy. Shoot apical meristem enlargement and altered
CC floral development. Reduced heterochromatin content, more open
CC conformation of euchromatin and dramatic increase of homologous
CC recombination. {ECO:0000269|PubMed:11163246}.
CC -!- SIMILARITY: Belongs to the WD repeat HIR1 family. {ECO:0000305}.
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DR EMBL; AB027230; BAA77766.1; -; Genomic_DNA.
DR EMBL; AB010076; BAB11430.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97929.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97930.1; -; Genomic_DNA.
DR EMBL; AF375435; AAK53019.1; -; mRNA.
DR EMBL; AY074827; AAL69523.1; -; mRNA.
DR RefSeq; NP_568990.1; NM_125859.4. [Q9SXY1-2]
DR RefSeq; NP_974991.1; NM_203262.3. [Q9SXY1-1]
DR AlphaFoldDB; Q9SXY1; -.
DR SMR; Q9SXY1; -.
DR IntAct; Q9SXY1; 1.
DR STRING; 3702.AT5G64630.2; -.
DR PaxDb; Q9SXY1; -.
DR PRIDE; Q9SXY1; -.
DR ProteomicsDB; 222446; -. [Q9SXY1-1]
DR EnsemblPlants; AT5G64630.1; AT5G64630.1; AT5G64630. [Q9SXY1-2]
DR EnsemblPlants; AT5G64630.2; AT5G64630.2; AT5G64630. [Q9SXY1-1]
DR GeneID; 836584; -.
DR Gramene; AT5G64630.1; AT5G64630.1; AT5G64630. [Q9SXY1-2]
DR Gramene; AT5G64630.2; AT5G64630.2; AT5G64630. [Q9SXY1-1]
DR KEGG; ath:AT5G64630; -.
DR Araport; AT5G64630; -.
DR TAIR; locus:2174784; AT5G64630.
DR eggNOG; KOG1009; Eukaryota.
DR OMA; CTIAVRC; -.
DR OrthoDB; 685536at2759; -.
DR PhylomeDB; Q9SXY1; -.
DR PRO; PR:Q9SXY1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9SXY1; baseline and differential.
DR Genevisible; Q9SXY1; AT.
DR GO; GO:0033186; C:CAF-1 complex; IPI:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IMP:TAIR.
DR GO; GO:0006335; P:DNA replication-dependent chromatin assembly; IEA:InterPro.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IMP:TAIR.
DR GO; GO:0031507; P:heterochromatin assembly; IMP:TAIR.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR.
DR GO; GO:0006334; P:nucleosome assembly; IDA:TAIR.
DR GO; GO:0009555; P:pollen development; IGI:TAIR.
DR GO; GO:0010026; P:trichome differentiation; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR045145; PTHR15271.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR15271; PTHR15271; 1.
DR Pfam; PF00400; WD40; 4.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromatin regulator; DNA damage; DNA recombination;
KW DNA repair; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; WD repeat.
FT CHAIN 1..487
FT /note="Chromatin assembly factor 1 subunit FAS2"
FT /id="PRO_0000420141"
FT REPEAT 11..52
FT /note="WD 1"
FT REPEAT 62..101
FT /note="WD 2"
FT REPEAT 110..149
FT /note="WD 3"
FT REPEAT 152..191
FT /note="WD 4"
FT REPEAT 222..275
FT /note="WD 5"
FT REPEAT 295..335
FT /note="WD 6"
FT REPEAT 339..380
FT /note="WD 7"
FT REGION 387..487
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 387..421
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 432..462
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 383..397
FT /note="VGKKPVDGEEKKHDL -> GLLLVLSLLNLPEPD (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_044413"
FT VAR_SEQ 398..487
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_044414"
SQ SEQUENCE 487 AA; 54106 MW; 06CB34C29E1F3191 CRC64;
MKGGTIQISW HDGKPVLTVD FHPISGLLAT AGADYDIKLW LINSGQAEKK VPSVSYQSSL
TYHGCAVNTI RFSPSGELLA SGADGGELFI WKLHPSETNQ SWKVHKSLSF HRKDVLDLQW
SPDDAYLISG SVDNSCIIWD VNKGSVHQIL DAHCHYVQGV AWDPLAKYVA SLSSDRTCRI
YANKPQTKSK GVEKMNYVCQ HVIMKADQQR GDETKTIKTH LFHDETLPSF FRRLSWSPDG
SFLLIPAGSF KVSPTSEAVN ATYVFSRKDL SRPALQLPGA SKPVVVVRFC PVAFKLRGSS
SEEGFFKLPY RLVFAIATLN SVYIYDTECV APIAVLAGLH YAAITDITWS PNASYLALSS
QDGYCTLVEF EDKELGEAVS ISVGKKPVDG EEKKHDLEKG DELMTETTPD ESKKQAELEQ
NEESKQPLPS KITTDGKEKE HIMQKTDDEV MTETRHEEEN QPLQSKVNTP VSNKPARKRI
TPMAIDP
