FATA_VIBA7
ID FATA_VIBA7 Reviewed; 726 AA.
AC P11461; P19830;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Ferric-anguibactin receptor FatA {ECO:0000305};
DE AltName: Full=OM2 {ECO:0000303|PubMed:3968037};
DE AltName: Full=Outer-membrane receptor FatA {ECO:0000303|PubMed:17259629};
DE Flags: Precursor;
GN Name=fatA {ECO:0000303|PubMed:1748657};
OS Vibrio anguillarum (strain ATCC 68554 / 775) (Listonella anguillarum).
OG Plasmid pJM1.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=882102;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=ATCC 68554 / 775;
RX PubMed=2830268; DOI=10.1016/s0021-9258(18)69147-1;
RA Actis L.A., Tolmasky M.E., Farrell D.H., Crosa J.H.;
RT "Genetic and molecular characterization of essential components of the
RT Vibrio anguillarum plasmid-mediated iron-transport system.";
RL J. Biol. Chem. 263:2853-2860(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 68554 / 775;
RX PubMed=13129954; DOI=10.1128/jb.185.19.5822-5830.2003;
RA Di Lorenzo M., Stork M., Tolmasky M.E., Actis L.A., Farrell D., Welch T.J.,
RA Crosa L.M., Wertheimer A.M., Chen Q., Salinas P., Waldbeser L., Crosa J.H.;
RT "Complete sequence of virulence plasmid pJM1 from the marine fish pathogen
RT Vibrio anguillarum strain 775.";
RL J. Bacteriol. 185:5822-5830(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 68554 / 775;
RX PubMed=21576332; DOI=10.1128/iai.05138-11;
RA Naka H., Dias G.M., Thompson C.C., Dubay C., Thompson F.L., Crosa J.H.;
RT "Complete genome sequence of the marine fish pathogen Vibrio anguillarum
RT harboring the pJM1 virulence plasmid and genomic comparison with other
RT virulent strains of V. anguillarum and V. ordalii.";
RL Infect. Immun. 79:2889-2900(2011).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 637-726.
RC STRAIN=ATCC 68554 / 775;
RX PubMed=2311935; DOI=10.1016/0378-1119(90)90112-5;
RA Farrell D.H., Mikesell P., Actis L.A., Crosa J.H.;
RT "A regulatory gene, angR, of the iron uptake system of Vibrio anguillarum:
RT similarity with phage P22 cro and regulation by iron.";
RL Gene 86:45-51(1990).
RN [5]
RP SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=ATCC 68554 / 775;
RX PubMed=3968037; DOI=10.1128/jb.161.2.736-742.1985;
RA Actis L.A., Potter S.A., Crosa J.H.;
RT "Iron-regulated outer membrane protein OM2 of Vibrio anguillarum is encoded
RT by virulence plasmid pJM1.";
RL J. Bacteriol. 161:736-742(1985).
RN [6]
RP SUBUNIT.
RC STRAIN=ATCC 68554 / 775;
RX PubMed=1748657; DOI=10.1016/s0021-9258(18)54358-1;
RA Koester W.L., Actis L.A., Waldbeser L.S., Tolmasky M.E., Crosa J.H.;
RT "Molecular characterization of the iron transport system mediated by the
RT pJM1 plasmid in Vibrio anguillarum 775.";
RL J. Biol. Chem. 266:23829-23833(1991).
RN [7]
RP SUBUNIT.
RX PubMed=15557661; DOI=10.1128/iai.72.12.7326-7329.2004;
RA Stork M., Di Lorenzo M., Mourino S., Osorio C.R., Lemos M.L., Crosa J.H.;
RT "Two tonB systems function in iron transport in Vibrio anguillarum, but
RT only one is essential for virulence.";
RL Infect. Immun. 72:7326-7329(2004).
RN [8]
RP FUNCTION, FERRIC-ANGUIBACTIN BINDING, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP MUTAGENESIS OF ARG-130; GLY-131; LYS-165; GLY-166; GLY-173; GLY-178;
RP ARG-463; GLU-540; PHE-557 AND GLU-585.
RC STRAIN=ATCC 68554 / 775;
RX PubMed=17259629; DOI=10.1099/mic.0.2006/001735-0;
RA Lopez C.S., Alice A.F., Chakraborty R., Crosa J.H.;
RT "Identification of amino acid residues required for ferric-anguibactin
RT transport in the outer-membrane receptor FatA of Vibrio anguillarum.";
RL Microbiology 153:570-584(2007).
CC -!- FUNCTION: Involved in the uptake of iron in complex with the
CC siderophore anguibactin. Binds and transports ferric-anguibactin from
CC the cell surface to the periplasm. {ECO:0000269|PubMed:17259629,
CC ECO:0000269|PubMed:2830268}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=8 nM for ferric-anguibactin {ECO:0000269|PubMed:17259629};
CC -!- SUBUNIT: Part of an iron transport system composed of the outer
CC membrane receptor FatA, the periplasmic binding protein FatB and the
CC inner membrane proteins FatC and FatD (PubMed:1748657). Energy for the
CC transport is provided by the TonB2 system (PubMed:15557661).
CC {ECO:0000269|PubMed:15557661, ECO:0000305|PubMed:1748657}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000269|PubMed:3968037}.
CC -!- INDUCTION: Induced by iron-limiting conditions.
CC {ECO:0000269|PubMed:2830268, ECO:0000269|PubMed:3968037}.
CC -!- DISRUPTION PHENOTYPE: Mutants are unable to grow under iron-limiting
CC conditions in the presence of anguibactin.
CC {ECO:0000269|PubMed:2830268}.
CC -!- SIMILARITY: Belongs to the TonB-dependent receptor family.
CC {ECO:0000305}.
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DR EMBL; AY312585; AAR12527.1; -; Genomic_DNA.
DR EMBL; M34504; AAA79859.1; -; Genomic_DNA.
DR PIR; B29928; B29928.
DR RefSeq; NP_943552.1; NC_005250.1.
DR RefSeq; WP_011154638.1; NC_005250.1.
DR AlphaFoldDB; P11461; -.
DR SMR; P11461; -.
DR TCDB; 1.B.14.1.11; the outer membrane receptor (omr) family.
DR eggNOG; COG4774; Bacteria.
DR OMA; AEWDLPW; -.
DR Proteomes; UP000006800; Plasmid pJM1.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015344; F:siderophore uptake transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0038023; F:signaling receptor activity; IEA:InterPro.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR Gene3D; 2.170.130.10; -; 1.
DR Gene3D; 2.40.170.20; -; 1.
DR InterPro; IPR030149; FcuA/FatA.
DR InterPro; IPR012910; Plug_dom.
DR InterPro; IPR037066; Plug_dom_sf.
DR InterPro; IPR039423; TonB-dep_rcpt.
DR InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR InterPro; IPR010916; TonB_box_CS.
DR InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR InterPro; IPR010917; TonB_rcpt_CS.
DR InterPro; IPR010105; TonB_sidphr_rcpt.
DR PANTHER; PTHR32552; PTHR32552; 1.
DR PANTHER; PTHR32552:SF82; PTHR32552:SF82; 1.
DR Pfam; PF07715; Plug; 1.
DR Pfam; PF00593; TonB_dep_Rec; 1.
DR TIGRFAMs; TIGR01783; TonB-siderophor; 1.
DR PROSITE; PS00430; TONB_DEPENDENT_REC_1; 1.
DR PROSITE; PS01156; TONB_DEPENDENT_REC_2; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Ion transport; Iron; Iron transport; Membrane;
KW Plasmid; Receptor; Signal; TonB box; Transmembrane;
KW Transmembrane beta strand; Transport.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..726
FT /note="Ferric-anguibactin receptor FatA"
FT /id="PRO_0000034752"
FT MOTIF 49..56
FT /note="TonB box"
FT MOTIF 709..726
FT /note="TonB C-terminal box"
FT MUTAGEN 130
FT /note="R->A,Q: Loss of activity."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 131
FT /note="G->A: 6-fold decrease in Vmax. Does not affect Km
FT for ferric-anguibactin."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 165
FT /note="K->A,Q: Loss of activity."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 166
FT /note="G->A: 9-fold decrease in Vmax. 5-fold increase in Km
FT for ferric-anguibactin."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 173
FT /note="G->A: 2-fold decrease in Vmax. 3-fold increase in Km
FT for ferric-anguibactin."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 178
FT /note="G->A: No change in Vmax. 3-fold increase in Km for
FT ferric-anguibactin."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 463
FT /note="R->A,E: Loss of activity."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 540
FT /note="E->A: No change in Vmax. Does not affect Km for
FT ferric-anguibactin."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 540
FT /note="E->Q: 3-fold decrease in Vmax. Does not affect Km
FT for ferric-anguibactin."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 557
FT /note="F->A: 4-fold decrease in Vmax. Does not affect Km
FT for ferric-anguibactin."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 585
FT /note="E->A: Loss of activity."
FT /evidence="ECO:0000269|PubMed:17259629"
FT MUTAGEN 585
FT /note="E->Q: Almost no change in Vmax. Does not affect Km
FT for ferric-anguibactin."
FT /evidence="ECO:0000269|PubMed:17259629"
SQ SEQUENCE 726 AA; 78894 MW; 88B67A219395B154 CRC64;
MTHQVATCHK KQSFSGKPTL SRIALLVALQ ISASALPISI THAEEQADES ITVYGQANEA
YAAGKISKAS SIGMLGDKDF LDTPFNAIGY TDKHIQDQHA QDISDVISAS DPSVFTSGET
GLNKESFKIR GFSSDIGDVM FNGLYGIAPY YRSSPEMYQR IDVLKGPASL LNGMPPNGSV
GGSINLVTKR AQEAPITSFT GTYMSDSQFG GHIDIGRRFG ENEQFGVRFN GVFRDGDASV
DGQSRKAQLA SLSLDWRNDI ALIEADLYFS TERVDGPNRG LSIASGVDVP SPPSSDTLLS
PSWAYNDSED KGMMIRAELD LSNSVTAYGA VGASRTDFDS NVPQRVKIID DSGTLEVSLG
SVKLESKRTS GEVGIRSSFD TGPIEHYLVL NSTYFREDKN DSPTGNNNPG SWNPNIYNPV
WGPEDSTYDN YYELPVDSTQ ISFGVADTLS LANGKYQVTL GLRHQSIDYE SGVTWNGNAF
PTTKLKESTY TPAIVALYKV SDSVSLYGNY TEGLTNGKTA GSGAANVGEA FEPQKTKQTE
AGLKLDMNDF AHTFSLFEIK KPNGYQDPDT NIYSFGGEQR NRGIEWGFYG TVLEDYTLTG
GIAYTDAEIT KATDVTTEGK QATKLPDLQA KLALEWNLPV MRQLTLIGQA NYMSEQYIDA
QNTQSLSAQT IFDLGARYNS TIANQSVIWR LAVNNVTDEA YWTTTHYASL ALGAPRTVML
SATADF
