FATB_CUPHO
ID FATB_CUPHO Reviewed; 415 AA.
AC Q39513;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Palmitoyl-acyl carrier protein thioesterase, chloroplastic;
DE EC=3.1.2.-;
DE AltName: Full=16:0-acyl-carrier protein thioesterase;
DE Short=16:0-ACP thioesterase;
DE AltName: Full=Acyl-[acyl-carrier-protein] hydrolase;
DE AltName: Full=PATE;
DE Flags: Precursor;
GN Name=FATB1;
OS Cuphea hookeriana (Cigar plant).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Lythraceae; Cuphea.
OX NCBI_TaxID=36775;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Seed;
RX PubMed=7734968; DOI=10.2307/3869857;
RA Jones A., Davies H.M., Voelker T.A.;
RT "Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary
RT origin of plant acyl-ACP thioesterases.";
RL Plant Cell 7:359-371(1995).
CC -!- FUNCTION: Plays an essential role in chain termination during de novo
CC fatty acid synthesis. High thioesterase activity for palmitoyl-ACP
CC versus other acyl-ACPs.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + hexadecanoyl-[ACP] = H(+) + hexadecanoate + holo-[ACP];
CC Xref=Rhea:RHEA:41932, Rhea:RHEA-COMP:9652, Rhea:RHEA-COMP:9685,
CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78483;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the acyl-ACP thioesterase family. {ECO:0000305}.
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DR EMBL; U17076; AAC48990.1; -; mRNA.
DR AlphaFoldDB; Q39513; -.
DR SMR; Q39513; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0016296; F:palmitoyl-[acyl-carrier-protein] hydrolase activity; IEA:RHEA.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR021113; Acyl-ACP-thioesterase_N.
DR InterPro; IPR002864; Acyl-ACP_thioesterase.
DR InterPro; IPR045023; FATA/B.
DR InterPro; IPR029069; HotDog_dom_sf.
DR PANTHER; PTHR31727; PTHR31727; 1.
DR Pfam; PF01643; Acyl-ACP_TE; 1.
DR Pfam; PF12590; Acyl-thio_N; 1.
DR SUPFAM; SSF54637; SSF54637; 2.
PE 2: Evidence at transcript level;
KW Chloroplast; Fatty acid biosynthesis; Fatty acid metabolism; Hydrolase;
KW Lipid biosynthesis; Lipid metabolism; Plastid; Transit peptide.
FT TRANSIT 1..60
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 61..415
FT /note="Palmitoyl-acyl carrier protein thioesterase,
FT chloroplastic"
FT /id="PRO_0000000593"
FT REGION 1..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 314
FT /evidence="ECO:0000255"
FT ACT_SITE 316
FT /evidence="ECO:0000255"
FT ACT_SITE 351
FT /evidence="ECO:0000255"
SQ SEQUENCE 415 AA; 45939 MW; A50974B5A2642CFE CRC64;
MVATAASSAF FPLPSADTSS RPGKLGNKPS SLSPLKPKST PNGGLQVKAN ASAPPKINGS
PVGLKSGGLK TQEDAHSAPP PRTFINQLPD WSMLLAAITT VFLAAEKQWM MLDWKPKRPD
MLVDPFGLGS IVQDGLVFRQ NFSIRSYEIG ADRTASIETV MNHLQETALN HVKIAGLSND
GFGRTPEMYK RDLIWVVAKM QVMVNRYPTW GDTVEVNTWV AKSGKNGMRR DWLISDCNTG
EILTRASSVW VMMNQKTRRL SKIPDEVRNE IEPHFVDSPP VIEDDDRKLP KLDEKTADSI
RKGLTPRWND LDVNQHVNNV KYIGWILEST PPEVLETQEL CSLTLEYRRE CGRESVLESL
TAMDPSGGGY GSQFQHLLRL EDGGEIVKGR TEWRPKNGVI NGVVPTGESS PGDYS
