ID   FAU1_THEKO              Reviewed;         471 AA.
AC   Q5JHN3;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Probable ribonuclease FAU-1 {ECO:0000255|HAMAP-Rule:MF_01910, ECO:0000305};
DE            EC=3.1.26.- {ECO:0000255|HAMAP-Rule:MF_01910, ECO:0000269|PubMed:28978920};
DE   AltName: Full=RNA-binding protein FAU-1 {ECO:0000255|HAMAP-Rule:MF_01910};
GN   Name=fau-1 {ECO:0000255|HAMAP-Rule:MF_01910, ECO:0000303|PubMed:28978920};
GN   OrderedLocusNames=TK2227;
OS   Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS   (Pyrococcus kodakaraensis (strain KOD1)).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Thermococcus.
OX   NCBI_TaxID=69014;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX   PubMed=15710748; DOI=10.1101/gr.3003105;
RA   Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT   "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT   kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL   Genome Res. 15:352-363(2005).
RN   [2]
RP   FUNCTION AS A RNASE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX   PubMed=28978920; DOI=10.1038/s41598-017-13062-3;
RA   Ikeda Y., Okada Y., Sato A., Kanai T., Tomita M., Atomi H., Kanai A.;
RT   "An archaeal RNA binding protein, FAU-1, is a novel ribonuclease related to
RT   rRNA stability in Pyrococcus and Thermococcus.";
RL   Sci. Rep. 7:12674-12674(2017).
CC   -!- FUNCTION: Probable RNase involved in rRNA stability through maturation
CC       and/or degradation of precursor rRNAs. Preferentially cleaves UA
CC       sequences in the 5' precursor region of 5S rRNA (PubMed:28978920).
CC       Binds to RNA in loop regions with AU-rich sequences (By similarity).
CC       {ECO:0000250|UniProtKB:Q8U4Q7, ECO:0000269|PubMed:28978920}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant shows a delay of exponential
CC       phase, reduction of maximum cell number and significant changes in the
CC       nucleotide sequence lengths of its 5S, 16S, and 23S rRNAs in early
CC       exponential phase. {ECO:0000269|PubMed:28978920}.
CC   -!- SIMILARITY: Belongs to the FAU-1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01910, ECO:0000305}.
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DR   EMBL; AP006878; BAD86416.1; -; Genomic_DNA.
DR   RefSeq; WP_011251177.1; NC_006624.1.
DR   AlphaFoldDB; Q5JHN3; -.
DR   SMR; Q5JHN3; -.
DR   STRING; 69014.TK2227; -.
DR   EnsemblBacteria; BAD86416; BAD86416; TK2227.
DR   GeneID; 3235430; -.
DR   KEGG; tko:TK2227; -.
DR   PATRIC; fig|69014.16.peg.2182; -.
DR   eggNOG; arCOG04307; Archaea.
DR   HOGENOM; CLU_044303_0_0_2; -.
DR   InParanoid; Q5JHN3; -.
DR   OMA; GTYVNVC; -.
DR   OrthoDB; 83570at2157; -.
DR   PhylomeDB; Q5JHN3; -.
DR   Proteomes; UP000000536; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016891; F:endoribonuclease activity, producing 5'-phosphomonoesters; IEA:UniProtKB-UniRule.
DR   GO; GO:0035925; F:mRNA 3'-UTR AU-rich region binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IBA:GO_Central.
DR   GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR   Gene3D; 2.40.380.10; -; 1.
DR   HAMAP; MF_01910; RNA_binding_AU_1; 1.
DR   InterPro; IPR007295; DUF402.
DR   InterPro; IPR035930; FomD-like_sf.
DR   InterPro; IPR019307; RNA-bd_AU-1/RNase_E/G.
DR   InterPro; IPR016730; RNA-bd_FAU-1.
DR   InterPro; IPR022967; S1_dom.
DR   Pfam; PF04167; DUF402; 1.
DR   Pfam; PF10150; RNase_E_G; 1.
DR   PIRSF; PIRSF018644; RNA-binding_FAU-1; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF159234; SSF159234; 1.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; RNA-binding;
KW   rRNA processing.
FT   CHAIN           1..471
FT                   /note="Probable ribonuclease FAU-1"
FT                   /id="PRO_0000334209"
SQ   SEQUENCE   471 AA;  54070 MW;  C86A1675A4EE7A98 CRC64;
     MSTDTRPTVR IRGIYSTALT KLFLDRGFGI SQPSNKIVER FGLEKTYDDF DVDIYDKKDR
     HGVVLVGDAV EEAKKVLEEE LIDVFFRRLP YQLYGIYKGL VVKIDEKYVY VDLGSAIGTI
     PRKDLPKAVE GDEILVQVKK HNLLPHLSTT LTIPGDYAVL IPKPIGAQRH VKISRKIRDN
     QERERLRILG LSIDLGKWGV LWRTAAAYKD WNLLRDEIVR LSKLADRLAK ADTYFAPSLI
     IEGRNIYEVE FGGGAKRKLD EIRNKVVPTV DGHHQLKAKD PELGFAVEIA EGILSKVPGQ
     RVKVNQGFWE ALVENKGPKK GWLFTLEHVK PDGQRIRLGP GEVLEVSHNP LRVTIKRHLK
     PGKFYDGLEL PIEFGDYAIT ELEAGKWWFV HRYYDKNGNL KGEYYNINTP VEIYPDGARY
     VDLEVDIVKW PDGKKEIIDQ EELKEHYEEG IISEKLYRAV LRITQEVFER V