FAX2_ARATH
ID FAX2_ARATH Reviewed; 240 AA.
AC Q94A32; Q8LC37; Q9M238;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Protein FATTY ACID EXPORT 2, chloroplastic {ECO:0000303|PubMed:25646734};
DE Short=At-FAX2 {ECO:0000303|PubMed:25646734};
DE Flags: Precursor;
GN Name=FAX2 {ECO:0000303|PubMed:25646734};
GN OrderedLocusNames=At3g43520 {ECO:0000312|Araport:AT3G43520};
GN ORFNames=T18D12.90 {ECO:0000312|EMBL:CAB81792.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAK91438.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, NOMENCLATURE, AND DEVELOPMENTAL STAGE.
RX PubMed=25646734; DOI=10.1371/journal.pbio.1002053;
RA Li N., Guegel I.L., Giavalisco P., Zeisler V., Schreiber L., Soll J.,
RA Philippar K.;
RT "FAX1, a novel membrane protein mediating plastid fatty acid export.";
RL PLoS Biol. 13:E1002053-E1002053(2015).
CC -!- FUNCTION: May be involved in free fatty acids export from the plastids.
CC {ECO:0000250|UniProtKB:Q93V66}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed during seed development and
CC germination. {ECO:0000305|PubMed:25646734}.
CC -!- MISCELLANEOUS: For all TMEM14 proteins, 4 hydrophobic alpha-helical
CC domains are predicted. However, NMR structure determination of the
CC human TMEM14A showed that only 3 of these helices are membrane-spaning
CC while the amphiphilic N-terminal helix is probably located at the lipid
CC micelle-water interface. {ECO:0000305|PubMed:25646734}.
CC -!- SIMILARITY: Belongs to the TMEM14 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB81792.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL138644; CAB81792.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77802.1; -; Genomic_DNA.
DR EMBL; AY050422; AAK91438.1; -; mRNA.
DR EMBL; BT000533; AAN18102.1; -; mRNA.
DR EMBL; AY086820; AAM63868.1; -; mRNA.
DR PIR; T47394; T47394.
DR RefSeq; NP_566866.1; NM_114220.4.
DR AlphaFoldDB; Q94A32; -.
DR IntAct; Q94A32; 7.
DR STRING; 3702.AT3G43520.1; -.
DR TCDB; 2.A.126.1.16; the fatty acid exporter (fax) family.
DR PaxDb; Q94A32; -.
DR PRIDE; Q94A32; -.
DR ProteomicsDB; 230960; -.
DR EnsemblPlants; AT3G43520.1; AT3G43520.1; AT3G43520.
DR GeneID; 823438; -.
DR Gramene; AT3G43520.1; AT3G43520.1; AT3G43520.
DR KEGG; ath:AT3G43520; -.
DR Araport; AT3G43520; -.
DR TAIR; locus:2098189; AT3G43520.
DR eggNOG; KOG4267; Eukaryota.
DR HOGENOM; CLU_078925_1_0_1; -.
DR InParanoid; Q94A32; -.
DR OMA; IVLPHFR; -.
DR OrthoDB; 1642026at2759; -.
DR PRO; PR:Q94A32; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q94A32; baseline and differential.
DR Genevisible; Q94A32; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR Gene3D; 1.10.10.1740; -; 1.
DR InterPro; IPR005349; TMEM14.
DR InterPro; IPR044890; TMEM14_sf.
DR PANTHER; PTHR12668; PTHR12668; 1.
DR Pfam; PF03647; Tmemb_14; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..84
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 85..240
FT /note="Protein FATTY ACID EXPORT 2, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000432802"
FT TRANSMEM 164..184
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT REGION 97..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 22
FT /note="H -> L (in Ref. 4; AAM63868)"
FT /evidence="ECO:0000305"
FT CONFLICT 92
FT /note="I -> N (in Ref. 4; AAM63868)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 240 AA; 24767 MW; 7075C408A19E4A53 CRC64;
MADLILSSSS AQSSLLHVRP NHYLLNTSTA SPIRSVRFQN SNGFHPLAFA SGHRRLPLGA
VVPSDSTKTI TSTITANCVD SGVKAVEVEP TIDYGGGGGI GGDKFGGGGG GGDGNDDGGE
DDKEESDGKK STPLSMSQKL TLGYAFLVGV GGLMGYLKSG SQKSLLAGGL SAAVLLYVFS
QLPTKPVLAS TVGVVMAGAL MYVMGTRYMR SKKIFPAGVV SIMSFIMTGG YIHGIMRSLH
