FAX4_ARATH
ID FAX4_ARATH Reviewed; 177 AA.
AC Q8LPG1; B3LFC0; Q3ED26; Q9C880;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Protein FATTY ACID EXPORT 4, chloroplastic {ECO:0000303|PubMed:25646734};
DE Short=At-FAX4 {ECO:0000303|PubMed:25646734};
DE Flags: Precursor;
GN Name=FAX4 {ECO:0000303|PubMed:25646734};
GN OrderedLocusNames=At1g33265 {ECO:0000312|Araport:AT1G33265};
GN ORFNames=T16O9.6 {ECO:0000312|EMBL:AAG51288.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAM20721.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=25646734; DOI=10.1371/journal.pbio.1002053;
RA Li N., Guegel I.L., Giavalisco P., Zeisler V., Schreiber L., Soll J.,
RA Philippar K.;
RT "FAX1, a novel membrane protein mediating plastid fatty acid export.";
RL PLoS Biol. 13:E1002053-E1002053(2015).
CC -!- FUNCTION: May be involved in free fatty acids export from the plastids.
CC {ECO:0000250|UniProtKB:Q93V66}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: For all TMEM14 proteins, 4 hydrophobic alpha-helical
CC domains are predicted. However, NMR structure determination of the
CC human TMEM14A showed that only 3 of these helices are membrane-spaning
CC while the amphiphilic N-terminal helix is probably located at the lipid
CC micelle-water interface. {ECO:0000305|PubMed:25646734}.
CC -!- SIMILARITY: Belongs to the TMEM14 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51288.1; Type=Erroneous gene model prediction; Note=The predicted gene At1g33260 has been split into 2 genes: At1g33260 and At1g33265.; Evidence={ECO:0000305};
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DR EMBL; AC027035; AAG51288.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31577.1; -; Genomic_DNA.
DR EMBL; AY099870; AAM20721.1; -; mRNA.
DR EMBL; BT000332; AAN15651.1; -; mRNA.
DR RefSeq; NP_564422.1; NM_103055.4.
DR AlphaFoldDB; Q8LPG1; -.
DR STRING; 3702.AT1G33265.1; -.
DR PaxDb; Q8LPG1; -.
DR EnsemblPlants; AT1G33265.1; AT1G33265.1; AT1G33265.
DR GeneID; 840221; -.
DR Gramene; AT1G33265.1; AT1G33265.1; AT1G33265.
DR KEGG; ath:AT1G33265; -.
DR Araport; AT1G33265; -.
DR TAIR; locus:505006165; AT1G33265.
DR eggNOG; KOG1187; Eukaryota.
DR HOGENOM; CLU_130145_0_0_1; -.
DR OMA; GLFACTH; -.
DR OrthoDB; 1388088at2759; -.
DR PRO; PR:Q8LPG1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LPG1; baseline and differential.
DR Genevisible; Q8LPG1; AT.
DR GO; GO:0031969; C:chloroplast membrane; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IGI:TAIR.
DR GO; GO:1905885; P:positive regulation of triglyceride transport; IMP:TAIR.
DR Gene3D; 1.10.10.1740; -; 1.
DR InterPro; IPR005349; TMEM14.
DR InterPro; IPR044890; TMEM14_sf.
DR PANTHER; PTHR12668; PTHR12668; 1.
DR Pfam; PF03647; Tmemb_14; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..63
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 64..177
FT /note="Protein FATTY ACID EXPORT 4, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000432804"
FT TRANSMEM 85..105
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
SQ SEQUENCE 177 AA; 18433 MW; DEB0ED02C2D9A299 CRC64;
MWSLALTLPS PSLVSVRSTK LGRSVSNGGR NWSGLTKLSE KSKTKRGNGL CCKAELSELA
PVVSATYGVL LLGGGLFAYS KSGSKGSLFG GLTGSVLMAS AYFLTKSPET RVLGDTIGLG
AAFLFSSVFG FRLASSRKPV PAGPLLLLSI GMLSFFVMAY MHDSLPAISI PDPLPLP
