FAZ1_TRYB2
ID FAZ1_TRYB2 Reviewed; 1692 AA.
AC Q585H6; D6XFW0;
DT 14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Flagellar attachment zone protein 1 {ECO:0000303|PubMed:17945531};
GN Name=FAZ1 {ECO:0000303|PubMed:17945531}; ORFNames=Tb927.4.3740;
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431;
RN [1] {ECO:0000312|EMBL:AAX79229.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=17945531; DOI=10.1016/j.protis.2007.08.005;
RA Vaughan S., Kohl L., Ngai I., Wheeler R.J., Gull K.;
RT "A repetitive protein essential for the flagellum attachment zone filament
RT structure and function in Trypanosoma brucei.";
RL Protist 159:127-136(2008).
CC -!- FUNCTION: A component of FAZ filament that is required for correct FAZ
CC assembly and attachment. Not essential for new flagellum growth.
CC {ECO:0000269|PubMed:17945531}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:17945531}. Note=The flagellum attachment zone (FAZ)
CC is a complex structure comprising a FAZ filament structure, a
CC specialised microtubule quartet (MtQ) and the plasma membrane
CC attachment, a specialised area of flagellum.
CC {ECO:0000269|PubMed:17945531}.
CC -!- DISRUPTION PHENOTYPE: Assembly of a compromised FAZ and defects in
CC flagellum attachment and cytokinesis in procyclic trypanosomes
CC resulting in generation of 0N1K cells (zoids) and multinucleated cells,
CC demonstrates the role of an attached flagellum in cellular
CC morphogenesis. {ECO:0000269|PubMed:17945531}.
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DR EMBL; AC023489; AAX79229.1; -; Genomic_DNA.
DR EMBL; CP000067; AAZ10965.1; -; Genomic_DNA.
DR RefSeq; XP_844524.1; XM_839431.1.
DR AlphaFoldDB; Q585H6; -.
DR SMR; Q585H6; -.
DR PaxDb; Q585H6; -.
DR GeneID; 3656912; -.
DR KEGG; tbr:Tb927.4.3740; -.
DR VEuPathDB; TriTrypDB:Tb927.4.3740; -.
DR eggNOG; ENOG502QU7S; Eukaryota.
DR InParanoid; Q585H6; -.
DR OMA; HCLSMYT; -.
DR Proteomes; UP000008524; Chromosome 4.
DR GO; GO:0020016; C:ciliary pocket; ISO:GeneDB.
DR GO; GO:0005929; C:cilium; IDA:GeneDB.
DR GO; GO:0005856; C:cytoskeleton; IDA:GeneDB.
DR GO; GO:0120119; C:flagellum attachment zone; IDA:GeneDB.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR GO; GO:0060271; P:cilium assembly; IDA:GeneDB.
DR GO; GO:0000281; P:mitotic cytokinesis; IDA:GeneDB.
PE 4: Predicted;
KW Cell projection; Cilium; Coiled coil; Flagellum; Reference proteome;
KW Repeat.
FT CHAIN 1..1692
FT /note="Flagellar attachment zone protein 1"
FT /id="PRO_0000414606"
FT REPEAT 1012..1025
FT /note="1"
FT /evidence="ECO:0000255"
FT REPEAT 1026..1039
FT /note="2"
FT /evidence="ECO:0000255"
FT REPEAT 1040..1053
FT /note="3"
FT /evidence="ECO:0000255"
FT REPEAT 1054..1067
FT /note="4"
FT /evidence="ECO:0000255"
FT REPEAT 1068..1081
FT /note="5"
FT /evidence="ECO:0000255"
FT REPEAT 1082..1095
FT /note="6"
FT /evidence="ECO:0000255"
FT REPEAT 1096..1109
FT /note="7"
FT /evidence="ECO:0000255"
FT REPEAT 1110..1123
FT /note="8"
FT /evidence="ECO:0000255"
FT REPEAT 1124..1137
FT /note="9"
FT /evidence="ECO:0000255"
FT REPEAT 1138..1151
FT /note="10"
FT /evidence="ECO:0000255"
FT REPEAT 1152..1165
FT /note="11"
FT /evidence="ECO:0000255"
FT REPEAT 1166..1179
FT /note="12"
FT /evidence="ECO:0000255"
FT REPEAT 1180..1193
FT /note="13"
FT /evidence="ECO:0000255"
FT REPEAT 1194..1207
FT /note="14"
FT /evidence="ECO:0000255"
FT REPEAT 1208..1221
FT /note="15"
FT /evidence="ECO:0000255"
FT REPEAT 1222..1235
FT /note="16"
FT /evidence="ECO:0000255"
FT REPEAT 1236..1249
FT /note="17"
FT /evidence="ECO:0000255"
FT REPEAT 1250..1263
FT /note="18"
FT /evidence="ECO:0000255"
FT REPEAT 1264..1277
FT /note="19"
FT /evidence="ECO:0000255"
FT REPEAT 1278..1291
FT /note="20"
FT /evidence="ECO:0000255"
FT REPEAT 1292..1305
FT /note="21"
FT /evidence="ECO:0000255"
FT REPEAT 1306..1319
FT /note="22"
FT /evidence="ECO:0000255"
FT REPEAT 1320..1333
FT /note="23"
FT /evidence="ECO:0000255"
FT REPEAT 1334..1347
FT /note="24"
FT /evidence="ECO:0000255"
FT REPEAT 1348..1361
FT /note="25"
FT /evidence="ECO:0000255"
FT REPEAT 1362..1375
FT /note="26"
FT /evidence="ECO:0000255"
FT REPEAT 1376..1389
FT /note="27"
FT /evidence="ECO:0000255"
FT REPEAT 1390..1403
FT /note="28"
FT /evidence="ECO:0000255"
FT REPEAT 1404..1417
FT /note="29"
FT /evidence="ECO:0000255"
FT REPEAT 1418..1431
FT /note="30"
FT /evidence="ECO:0000255"
FT REPEAT 1432..1445
FT /note="31"
FT /evidence="ECO:0000255"
FT REPEAT 1446..1459
FT /note="32"
FT /evidence="ECO:0000255"
FT REPEAT 1460..1473
FT /note="33"
FT /evidence="ECO:0000255"
FT REPEAT 1474..1487
FT /note="34"
FT /evidence="ECO:0000255"
FT REPEAT 1488..1501
FT /note="35"
FT /evidence="ECO:0000255"
FT REPEAT 1502..1515
FT /note="36"
FT /evidence="ECO:0000255"
FT REPEAT 1516..1529
FT /note="37"
FT /evidence="ECO:0000255"
FT REGION 1012..1529
FT /note="37 X 14 AA tandem repeats of E-E-L-E-L-K-[VA]-A-E-N-
FT E-K-L-A"
FT /evidence="ECO:0000255"
FT COILED 613..657
FT /evidence="ECO:0000255"
FT COILED 684..864
FT /evidence="ECO:0000255"
FT COILED 903..1607
FT /evidence="ECO:0000255"
SQ SEQUENCE 1692 AA; 192570 MW; 8ABB32A892D144C0 CRC64;
MALLNVISEN PLTLQPGQVI AFDHLANGEH WQWALGTVVS SDKHVVVVEQ WAVNEGSCET
LKHNISSEIQ KEMKRMGVFQ EQLSSARDKL AAIRSENEDR VSAARAVFED AKARVASVDE
VHMREVTSQA CPSPVAVEVL KSVLALAQND PTVTNCSTWD DIRMEYRRPN AIADFISADI
TGKTYPNAEE ICSSLNEQRL SSLAASRDSE AISSLHHWVL SALAYQEAYC RLTTDTRVQE
QNDAIANCIA GMKGCRLKVM KLKEELERGG TPTFGGQLTS FTKTSVQLKA PLSSVISIVG
VDPSAQDCVL TDDEVGLILD KAEQTRLQIN DHFSHLSNSY MEAMAELHCL SMYTSELEER
RLNLQERFVF SLFTNAGKTN APRRERIETD VGLRSVEAPR GDSANNIKDL QEIIKELSSH
DERWMYRNEP TVTTKHRKSY PGREWSKVVE RKPEELLSTF RTEQAAACHV PEDAIRNIEF
TATSEKLQVS FDVQHPVKQT AAEINKRLQE FPSRGMDRML CDVDQPKKGL DRAIVEVCRA
FDLREHAFRG MTFDKFIEEV AMKGRVGDKD AYESEIGDLL MLLDKIHNEN RSLQYTLEKS
AEEFRRQTAS TMREQESLRQ RNGELHAEIG RLRDLVEKLR DLADNQASEL ELLKLQKTQA
NQIRAQRNLS TFRGDDTAEP VYCVTLDELR EQTEHCDQVE RELERQREQC QNLLNAQDDL
LAELSGVSEE KEKLEAECER LEAELRQMEE KSRLSEQGLS EMTQRLEEKQ AEIEGLLENL
EQLDEQLEAL RAAEKSAQAH IEARDREISD LQQRLEGEID DHIKTTALLE ELRKHYNNLE
ELFDKQEAEL MAYREKRQNA HKVRSLEPTL RPIGTQTKPF QEMVSADEIS SEPLLSVTLD
EYNDHMHRSN QFQQENDLLR QQLQQANDER ENLHDRLEQL MAENQSLSEQ LHNMHEELER
EERDRSGVTL QNERLAEEIQ RKTAENEQLV LENNKSRSDI RNLNVQVQRL MEELELKAAE
NEKLAEELEL KAAENEKLAE ELELKVAENE KLAEELELKV AENEKLAEEL ELKAAENEKL
AEELELKAAE NEKLAEELEL KAAENEKLAE ELELKAAENE KLAEELELKA AENEKLAEEL
ELKAAENEKL AEELELKAAE NEKLAEELEL KVAENEKLAE ELELKAAENE KLAEELELKV
AENEKLAEEL ELKAAENEKL AEELELKAAE NEKLAEELEL KAAENEKLAE ELELKAAENE
KLAEELELKV AENEKLAEEL ELKAAENEKL AEELELKVAE NEKLAEELEL KAAENEKLAE
ELELKVAENE KLAEELELKA AENEKLAEEL ELKVAENEKL AEELELKAAE NEKLAEELEL
KAAENEKLAE ELELKAAENE KLAEELELKA AENEKLAEEL ELKAAENEKL AEELELKVAE
NEKLAEELEL KAAENEKLAE ELELKVAENE KLAEELELKA AENEKLAEEL ELKAAENEKL
AEELELKAAE NEKLAEELEL KVAENKRLAE EVTQRLSEKE LLAEDTSARL LEADSANSAL
QCKVKHLEEK LTLLSSEKET ALATLEAEIV DLLTQLKGLN GTNSALESLC ASKEKELVFL
REHCELWTDP TTKKEKVITR HVKVFDGNEW MKLITDRPEA LMSAFVIDAG NACHVPGDQI
HEVSFLNNKE KH
