FB309_ARATH
ID FB309_ARATH Reviewed; 453 AA.
AC Q9SZ44; B9DFP9; Q8W4N4;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=F-box protein At4g27050;
GN OrderedLocusNames=At4g27050; ORFNames=F10M23.390;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP INTERACTION WITH ASK4.
RX PubMed=12169662; DOI=10.1073/pnas.162339999;
RA Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D.;
RT "The F-box subunit of the SCF E3 complex is encoded by a diverse
RT superfamily of genes in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex.
CC {ECO:0000250}.
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins
CC (By similarity). Interacts with ASK4. {ECO:0000250}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL035440; CAB36551.1; -; Genomic_DNA.
DR EMBL; AL161566; CAB79560.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85291.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85292.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85293.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85294.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM67140.1; -; Genomic_DNA.
DR EMBL; AY062462; AAL32540.1; -; mRNA.
DR EMBL; BT000114; AAN15433.1; -; mRNA.
DR EMBL; AK316857; BAH19566.1; -; mRNA.
DR PIR; T04828; T04828.
DR RefSeq; NP_001031729.1; NM_001036652.1.
DR RefSeq; NP_001078451.1; NM_001084982.1.
DR RefSeq; NP_001328988.1; NM_001341836.1.
DR RefSeq; NP_194435.1; NM_118839.4.
DR RefSeq; NP_974624.1; NM_202895.2.
DR AlphaFoldDB; Q9SZ44; -.
DR BioGRID; 14100; 2.
DR IntAct; Q9SZ44; 1.
DR STRING; 3702.AT4G27050.2; -.
DR PaxDb; Q9SZ44; -.
DR PRIDE; Q9SZ44; -.
DR ProteomicsDB; 230083; -.
DR EnsemblPlants; AT4G27050.1; AT4G27050.1; AT4G27050.
DR EnsemblPlants; AT4G27050.2; AT4G27050.2; AT4G27050.
DR EnsemblPlants; AT4G27050.3; AT4G27050.3; AT4G27050.
DR EnsemblPlants; AT4G27050.4; AT4G27050.4; AT4G27050.
DR EnsemblPlants; AT4G27050.5; AT4G27050.5; AT4G27050.
DR GeneID; 828813; -.
DR Gramene; AT4G27050.1; AT4G27050.1; AT4G27050.
DR Gramene; AT4G27050.2; AT4G27050.2; AT4G27050.
DR Gramene; AT4G27050.3; AT4G27050.3; AT4G27050.
DR Gramene; AT4G27050.4; AT4G27050.4; AT4G27050.
DR Gramene; AT4G27050.5; AT4G27050.5; AT4G27050.
DR KEGG; ath:AT4G27050; -.
DR Araport; AT4G27050; -.
DR TAIR; locus:2116362; AT4G27050.
DR HOGENOM; CLU_010721_7_1_1; -.
DR InParanoid; Q9SZ44; -.
DR OMA; AGNHEDY; -.
DR OrthoDB; 816963at2759; -.
DR PhylomeDB; Q9SZ44; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9SZ44; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SZ44; baseline and differential.
DR Genevisible; Q9SZ44; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR006566; FBD.
DR InterPro; IPR013101; Leu-rich_rpt_2.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF00646; F-box; 1.
DR Pfam; PF07723; LRR_2; 1.
DR SMART; SM00579; FBD; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..453
FT /note="F-box protein At4g27050"
FT /id="PRO_0000281973"
FT DOMAIN 3..51
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT CONFLICT 216
FT /note="K -> R (in Ref. 3; AAL32540/AAN15433)"
FT /evidence="ECO:0000305"
FT CONFLICT 370
FT /note="C -> R (in Ref. 4; BAH19566)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 453 AA; 51301 MW; C6FC6FB6AED5DFCD CRC64;
MDTDLISNLP DDVLGKILSL VPTKLAAATS VLSKRWRNLL PLVDSLDFDE TMLFYPDNKD
GEDEASSYES GQRRRHSFSH FVDKTLALLS NAPLTEKFSL KCYHEDDGAR INRWIRTALE
RGCLELNLEA ANYLPIDSQY FTSNTLVKLT ISDGFYPGGH LLPFGGVFFP ALKTLTLVSV
CFTSVEMINF FIHGCPALEE LFLCVWMQYM SSPNVKRVTI TSDFDDDIQR PSMVLKTPSL
VYLDYSSYVA GNYYVVLDSL VEARLDLRLY EPIIYDEDGL WNAEVFGNIT NLIEAIRTIK
ILHLSPSSLE VFYYCCDLPV LEDLVNLSIE SDKEKGWEVM PRLLNKSPNL QTLVVKGLVH
RVTYCCGDAC ACIRMKDREI VSCLSRCRVK VLKILGYGGS FRELKQMRHF LGKLKCLETV
KVGVKEGSKK SNYLVANVMA LPRVSSKCKI QFI
