FB310_ARATH
ID FB310_ARATH Reviewed; 535 AA.
AC Q8GYP8; Q9FXB3;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=F-box protein At1g56610;
GN OrderedLocusNames=At1g56610; ORFNames=F25P12.94;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP INTERACTION WITH ASK4.
RX PubMed=12169662; DOI=10.1073/pnas.162339999;
RA Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D.;
RT "The F-box subunit of the SCF E3 complex is encoded by a diverse
RT superfamily of genes in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex (By
CC similarity). Interacts with ASK4. {ECO:0000250,
CC ECO:0000269|PubMed:12169662}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8GYP8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8GYP8-2; Sequence=VSP_037360;
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC {ECO:0000250}.
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DR EMBL; AC009323; AAG09102.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33414.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33415.1; -; Genomic_DNA.
DR EMBL; AK117458; BAC42123.1; -; mRNA.
DR PIR; H96607; H96607.
DR RefSeq; NP_001031201.1; NM_001036124.2. [Q8GYP8-2]
DR RefSeq; NP_176054.2; NM_104538.3. [Q8GYP8-1]
DR AlphaFoldDB; Q8GYP8; -.
DR BioGRID; 27341; 1.
DR PaxDb; Q8GYP8; -.
DR PRIDE; Q8GYP8; -.
DR ProteomicsDB; 230977; -. [Q8GYP8-1]
DR EnsemblPlants; AT1G56610.1; AT1G56610.1; AT1G56610. [Q8GYP8-1]
DR EnsemblPlants; AT1G56610.2; AT1G56610.2; AT1G56610. [Q8GYP8-2]
DR GeneID; 842116; -.
DR Gramene; AT1G56610.1; AT1G56610.1; AT1G56610. [Q8GYP8-1]
DR Gramene; AT1G56610.2; AT1G56610.2; AT1G56610. [Q8GYP8-2]
DR KEGG; ath:AT1G56610; -.
DR Araport; AT1G56610; -.
DR TAIR; locus:2027539; AT1G56610.
DR HOGENOM; CLU_010721_7_4_1; -.
DR InParanoid; Q8GYP8; -.
DR OrthoDB; 816963at2759; -.
DR PhylomeDB; Q8GYP8; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q8GYP8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GYP8; baseline and differential.
DR Genevisible; Q8GYP8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR006566; FBD.
DR InterPro; IPR013101; Leu-rich_rpt_2.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF07723; LRR_2; 1.
DR SMART; SM00579; FBD; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Ubl conjugation pathway.
FT CHAIN 1..535
FT /note="F-box protein At1g56610"
FT /id="PRO_0000375241"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 73..119
FT /note="F-box; degenerate"
FT VAR_SEQ 1..63
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_037360"
SQ SEQUENCE 535 AA; 61762 MW; A2127C2A21AA3565 CRC64;
MGFALVLIFL FGFYLFLMKS SSIFPQRPFT FLYDLWVLLL VLRFLFSLIQ ILILCLFNFR
FVEMTSNNKK KKTELCDLPK CLAPHILSWL PTKTAVTVSL LFMKGWWRSE MKNLSSLKFS
FSDDQEEEHF VRFVDQVLRQ RGNRKLDSFS LTLNDEIDGG FVTHLVDYPL DNGVEKLKLS
IYDIKGNFQL SSRVFSQATL VTLKLATNRS LIWINGDDVA AAHLPCLKTL WLDDVLVADV
KVFVRLLSRC PILEELVMID MKWHNWEACF VVSASLRRLK IVWTDYVEMD EYDRCPQSVL
FDTPNVLYLE YTDHIAGQYP LLKFSSLIEA KIRLEMIDEK EEEDEGQEVI VGDNATAFIT
GITSVRKLYL YANTIQVLHH YFDPPIPEFV YLTHLTIQSD KELGWDAIPE LLSKCPHLET
LVLEGLFHLA TDVCGDVCPC RRNMEQAISY LVKSPVTHLE IYEGVVGKKR GEVTEDAARF
GEQVRWFLMR MLHLQQVKIY GQTEDSVTAL YDIATELRRL EGKASPNVQI SVLQA
