FB331_ARATH
ID FB331_ARATH Reviewed; 359 AA.
AC Q9M365; F4JFG0;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 10-AUG-2010, sequence version 2.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Probable F-box protein At3g61730;
DE AltName: Full=Protein RFM;
DE AltName: Full=Reduced male fertility protein;
GN Name=RMF; OrderedLocusNames=At3g61730; ORFNames=F15G16.120, F21F14.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP INTERACTION WITH SKP1A, AND MUTAGENESIS OF ILE-96; LEU-100 AND ASP-101.
RX PubMed=20458496; DOI=10.1007/s00425-010-1178-x;
RA Kim O.K., Jung J.H., Park C.M.;
RT "An Arabidopsis F-box protein regulates tapetum degeneration and pollen
RT maturation during anther development.";
RL Planta 232:353-366(2010).
CC -!- FUNCTION: Regulates tapetum degeneration and pollen maturation during
CC anther development. {ECO:0000269|PubMed:20458496}.
CC -!- SUBUNIT: Interacts with SKP1A. {ECO:0000269|PubMed:20458496}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20458496}.
CC -!- TISSUE SPECIFICITY: Expressed in flower buds, developing anthers,
CC pollen grains, siliques, rosette leaves and roots. Detected at lower
CC levels in open flowers, stems and cauline leaves. Expressed in young
CC seedling in the hydathodes, shoot apical meristem, root tips and
CC lateral root primordia. {ECO:0000269|PubMed:20458496}.
CC -!- DEVELOPMENTAL STAGE: Expressed to a high level in young floral buds,
CC but decreases in the later floral developmental stages. Expression
CC restricted to microspores and pollen grains at the later developmental
CC stages of anther. {ECO:0000269|PubMed:20458496}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB71103.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL132959; CAB71103.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE80249.2; -; Genomic_DNA.
DR PIR; T47965; T47965.
DR RefSeq; NP_001319817.1; NM_001340124.1.
DR AlphaFoldDB; Q9M365; -.
DR STRING; 3702.AT3G61730.1; -.
DR iPTMnet; Q9M365; -.
DR PaxDb; Q9M365; -.
DR PRIDE; Q9M365; -.
DR EnsemblPlants; AT3G61730.1; AT3G61730.1; AT3G61730.
DR GeneID; 825346; -.
DR Gramene; AT3G61730.1; AT3G61730.1; AT3G61730.
DR KEGG; ath:AT3G61730; -.
DR Araport; AT3G61730; -.
DR eggNOG; ENOG502QV89; Eukaryota.
DR HOGENOM; CLU_051928_0_0_1; -.
DR InParanoid; Q9M365; -.
DR OMA; FRQKEKH; -.
DR OrthoDB; 921764at2759; -.
DR PhylomeDB; Q9M365; -.
DR PRO; PR:Q9M365; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M365; baseline and differential.
DR Genevisible; Q9M365; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0061458; P:reproductive system development; IBA:GO_Central.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..359
FT /note="Probable F-box protein At3g61730"
FT /id="PRO_0000396046"
FT DOMAIN 82..128
FT /note="F-box; degenerate"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..57
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 96
FT /note="I->S: Loss of interaction with SKP1A; when
FT associated with S-100 and A-101."
FT /evidence="ECO:0000269|PubMed:20458496"
FT MUTAGEN 100
FT /note="L->S: Loss of interaction with SKP1A; when
FT associated with S-96 and A-101."
FT /evidence="ECO:0000269|PubMed:20458496"
FT MUTAGEN 101
FT /note="D->A: Loss of interaction with SKP1A; when
FT associated with S-96 and S-100."
FT /evidence="ECO:0000269|PubMed:20458496"
SQ SEQUENCE 359 AA; 40452 MW; 7BFDD6797969B356 CRC64;
MKTRSSDAEG DSRGKMIAPV GEGNGGRKRK LVQSNEQKND IQREEDGRAK RRIVQSSDQK
NGKILRGIRG CVSPRCSAQT YQSRFSWYEQ DIWTYISRFL DGKSLVKLGA TNKWFYKIAM
EDTVWRFACL RDLQVPVPFP VSSTWIKIYA SAFDGSHSYL FRQKEKHIDW MRIGAFVLDS
RTSLLTESLS GRLKFPREGT IERMLQSSGS CLINDIKSGI WIADLQLVRC PVCDLSTCDG
TMQTLDARHI ELFLSEGYKD GSWDYNLIGS HKLQKNASAA CGAIFDLKHL KESSYSGILN
LKSWTGESND SQPKAVIAPH AVAVHTRLQE NEGILVKYHT MKAGTDGDIV SIRISQQLL
