FBF1_CAEEL
ID FBF1_CAEEL Reviewed; 614 AA.
AC Q9N5M6;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Fem-3 mRNA-binding factor 1;
GN Name=fbf-1; ORFNames=H12I13.4;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=9393998; DOI=10.1038/37297;
RA Zhang B., Gallegos M., Puoti A., Durkin E., Fields S., Kimble J.,
RA Wickens M.P.;
RT "A conserved RNA-binding protein that regulates sexual fates in the C.
RT elegans hermaphrodite germ line.";
RL Nature 390:477-484(1997).
RN [3]
RP INTERACTION WITH GLD-3.
RX PubMed=12431376; DOI=10.1016/s1534-5807(02)00322-2;
RA Eckmann C.R., Kraemer B., Wickens M., Kimble J.;
RT "GLD-3, a bicaudal-C homolog that inhibits FBF to control germline sex
RT determination in C. elegans.";
RL Dev. Cell 3:697-710(2002).
RN [4]
RP FUNCTION.
RX PubMed=15454534; DOI=10.1534/genetics.104.029264;
RA Eckmann C.R., Crittenden S.L., Suh N., Kimble J.;
RT "GLD-3 and control of the mitosis/meiosis decision in the germline of
RT Caenorhabditis elegans.";
RL Genetics 168:147-160(2004).
RN [5]
RP FUNCTION.
RX PubMed=16319922; DOI=10.1038/sj.emboj.7600901;
RA Lee M.H., Hook B., Lamont L.B., Wickens M., Kimble J.;
RT "LIP-1 phosphatase controls the extent of germline proliferation in
RT Caenorhabditis elegans.";
RL EMBO J. 25:88-96(2006).
RN [6]
RP FUNCTION.
RX PubMed=21455289; DOI=10.1371/journal.pgen.1001348;
RA Jeong J., Verheyden J.M., Kimble J.;
RT "Cyclin E and Cdk2 control GLD-1, the mitosis/meiosis decision, and
RT germline stem cells in Caenorhabditis elegans.";
RL PLoS Genet. 7:E1001348-E1001348(2011).
RN [7]
RP FUNCTION.
RX PubMed=22820175; DOI=10.1016/j.bbamcr.2012.07.006;
RA Cha D.S., Datla U.S., Hollis S.E., Kimble J., Lee M.H.;
RT "The Ras-ERK MAPK regulatory network controls dedifferentiation in
RT Caenorhabditis elegans germline.";
RL Biochim. Biophys. Acta 1823:1847-1855(2012).
CC -!- FUNCTION: Involved in the control of stem cells and sex determination
CC in the C.elegans hermaphrodite germline (PubMed:9393998). May also play
CC a role in the hermaphrodite germline proliferation and oogenesis
CC (PubMed:9393998, PubMed:15454534). Binds specifically to the regulatory
CC region of fem-3 3'-UTR and mediates the sperm/oocyte switch
CC (PubMed:9393998, PubMed:15454534). Negatively regulates gld-3
CC expression, possibly by directly binding to two sites within the 3'-UTR
CC of gld-3 isoform b (PubMed:9393998, PubMed:15454534). In association
CC with the cye-1/cdk-2 complex, negatively regulates gld-1 expression in
CC the distal germline cells of the mitotic zone (PubMed:21455289). By
CC binding to the 3'-UTR, represses phosphatase lip-1 expression in the
CC distal part of the germline mitotic zone (PubMed:16319922). Suppresses
CC germline tumor formation by preventing the dedifferentiation of
CC secondary spermatocytes (PubMed:22820175).
CC {ECO:0000269|PubMed:15454534, ECO:0000269|PubMed:16319922,
CC ECO:0000269|PubMed:21455289, ECO:0000269|PubMed:22820175,
CC ECO:0000269|PubMed:9393998}.
CC -!- SUBUNIT: Interacts (via C-terminus) with gld-3 isoform A in an RNA-
CC independent manner. {ECO:0000269|PubMed:12431376}.
CC -!- INTERACTION:
CC Q9N5M6; Q03571: cpb-1; NbExp=5; IntAct=EBI-1569950, EBI-316645;
CC Q9N5M6; Q95ZK7-1: gld-3; NbExp=2; IntAct=EBI-1569950, EBI-14989519;
CC Q9N5M6; O62486: nos-3; NbExp=5; IntAct=EBI-1569950, EBI-326004;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:9393998}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in the germline (at protein
CC level). {ECO:0000269|PubMed:9393998}.
CC -!- DEVELOPMENTAL STAGE: Expression increases in abundance during
CC postembryonic development and peaks during the fourth larval stage.
CC {ECO:0000269|PubMed:9393998}.
CC -!- MISCELLANEOUS: Fbf-1 and fbf-2 are >90% identical on both nucleotide
CC and protein sequence level. Experimental approaches often do not
CC distinguish between the two genes, which are collectively referred to
CC as fbf and are considered to be functionally redundant.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FO081202; CCD69857.1; -; Genomic_DNA.
DR RefSeq; NP_495216.1; NM_062815.6.
DR AlphaFoldDB; Q9N5M6; -.
DR SMR; Q9N5M6; -.
DR BioGRID; 39357; 10.
DR IntAct; Q9N5M6; 4.
DR STRING; 6239.H12I13.4; -.
DR EPD; Q9N5M6; -.
DR PaxDb; Q9N5M6; -.
DR PeptideAtlas; Q9N5M6; -.
DR EnsemblMetazoa; H12I13.4.1; H12I13.4.1; WBGene00001401.
DR GeneID; 174016; -.
DR KEGG; cel:CELE_H12I13.4; -.
DR UCSC; H12I13.4; c. elegans.
DR CTD; 174016; -.
DR WormBase; H12I13.4; CE20960; WBGene00001401; fbf-1.
DR eggNOG; KOG1488; Eukaryota.
DR GeneTree; ENSGT00970000196043; -.
DR HOGENOM; CLU_028494_1_0_1; -.
DR InParanoid; Q9N5M6; -.
DR OMA; DHANSFQ; -.
DR OrthoDB; 1364009at2759; -.
DR PhylomeDB; Q9N5M6; -.
DR PRO; PR:Q9N5M6; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00001401; Expressed in germ line (C elegans) and 3 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0043073; C:germ cell nucleus; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:WormBase.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:WormBase.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0051729; P:germline cell cycle switching, mitotic to meiotic cell cycle; IGI:WormBase.
DR GO; GO:0017148; P:negative regulation of translation; IGI:WormBase.
DR GO; GO:0008355; P:olfactory learning; IMP:WormBase.
DR GO; GO:0001555; P:oocyte growth; IGI:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:WormBase.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IGI:WormBase.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 8.
DR SMART; SM00025; Pumilio; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 8.
DR PROSITE; PS50303; PUM_HD; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Differentiation; Reference proteome;
KW Repeat; RNA-binding; Sexual differentiation.
FT CHAIN 1..614
FT /note="Fem-3 mRNA-binding factor 1"
FT /id="PRO_0000075932"
FT DOMAIN 160..564
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 185..223
FT /note="Pumilio 1"
FT REPEAT 224..263
FT /note="Pumilio 2"
FT REPEAT 269..305
FT /note="Pumilio 3"
FT REPEAT 306..342
FT /note="Pumilio 4"
FT REPEAT 343..382
FT /note="Pumilio 5"
FT REPEAT 398..434
FT /note="Pumilio 6"
FT REPEAT 436..471
FT /note="Pumilio 7"
FT REPEAT 483..519
FT /note="Pumilio 8"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 283..614
FT /note="Binding to gld-3 isoform A"
SQ SEQUENCE 614 AA; 70358 MW; 557B9D459F38C18C CRC64;
MDQSKMRYTN QFRKTPQKPT STEVGNHHTP AHSPMAQHET SMWNFNSLNP YFSMLNMNDG
MNYARHQQNH IVTSRPPTPL TDLMSLRSFQ SFPNVFMPVS RSRTSSFIQS DTDSSRLEPD
DFSQNVRYCS TEIDRNNSSS KNDHLKYSRP ALSRNSRSFT RSNNVLPTWS LDSNGEMRSR
LSLSEVLDSG DLMKFAVDKT GCQFLEKAVK GSLTSYQKFQ LFEQVIGRKD DFLKLSTNIF
GNYFVQEIIG MSLTTYDDDN IKRQEKLKNF ISSQMTDMCL DKFACRVIQS SLQNMDLSLA
CKLVQALPRD ARLIAICVDQ NANHVIQKVV AVIPLKNWEF IVDFVATPEH LRQICFDKYG
CRVVQTIIEK LTADSINVDL TSAAQHLRER ALQRLMTSVT NRCQELATNE YANYIIQHIV
SNDDLAVYRE CIIEKCLMRN LLSLSQEKFA SHVVEKAFLH APMELLAEMM DEIFDGYMPH
PGTGKDALDI MMFHQFGNYV VQCMLTICCD AVSGRRQTKE GSYDHANSFQ VWLKKLHSRV
TKERHRLSRF SSGKKMIETL AHLRSTHPIY GLQSSGHESF KTDCFSTASE HDGLELEKNG
IEEGNLRLMR TFSP
