FBIA_MYCS2
ID FBIA_MYCS2 Reviewed; 327 AA.
AC A0QTG2; I7G6J5;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Phosphoenolpyruvate transferase {ECO:0000255|HAMAP-Rule:MF_01257};
DE EC=2.7.8.28 {ECO:0000255|HAMAP-Rule:MF_01257};
DE AltName: Full=EPPG:FO PEP transferase {ECO:0000255|HAMAP-Rule:MF_01257};
GN Name=fbiA {ECO:0000255|HAMAP-Rule:MF_01257};
GN OrderedLocusNames=MSMEG_1830, MSMEI_1787;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
CC -!- FUNCTION: Catalyzes the transfer of the phosphoenolpyruvate moiety from
CC enoylpyruvoyl-2-diphospho-5'-guanosine (EPPG) to 7,8-didemethyl-8-
CC hydroxy-5-deazariboflavin (FO) with the formation of dehydro coenzyme
CC F420-0 and GMP. {ECO:0000255|HAMAP-Rule:MF_01257}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7,8-didemethyl-8-hydroxy-5-deazariboflavin + enolpyruvoyl-2-
CC diphospho-5'-guanosine = dehydro coenzyme F420-0 + GMP + H(+);
CC Xref=Rhea:RHEA:27510, ChEBI:CHEBI:15378, ChEBI:CHEBI:58115,
CC ChEBI:CHEBI:59904, ChEBI:CHEBI:143701, ChEBI:CHEBI:143705;
CC EC=2.7.8.28; Evidence={ECO:0000255|HAMAP-Rule:MF_01257};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01257};
CC -!- PATHWAY: Cofactor biosynthesis; coenzyme F420 biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_01257}.
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01257}.
CC -!- SIMILARITY: Belongs to the CofD family. {ECO:0000255|HAMAP-
CC Rule:MF_01257}.
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DR EMBL; CP000480; ABK71517.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP38259.1; -; Genomic_DNA.
DR RefSeq; WP_011727938.1; NZ_SIJM01000028.1.
DR RefSeq; YP_886200.1; NC_008596.1.
DR PDB; 6UVX; X-ray; 2.30 A; A/B=1-327.
DR PDB; 6UW1; X-ray; 2.21 A; A/B=1-327.
DR PDB; 6UW3; X-ray; 2.40 A; A/B=1-327.
DR PDB; 6UW5; X-ray; 2.20 A; A/B=1-327.
DR PDB; 6UW7; X-ray; 2.34 A; A/B=1-327.
DR PDBsum; 6UVX; -.
DR PDBsum; 6UW1; -.
DR PDBsum; 6UW3; -.
DR PDBsum; 6UW5; -.
DR PDBsum; 6UW7; -.
DR AlphaFoldDB; A0QTG2; -.
DR SMR; A0QTG2; -.
DR STRING; 246196.MSMEI_1787; -.
DR EnsemblBacteria; ABK71517; ABK71517; MSMEG_1830.
DR EnsemblBacteria; AFP38259; AFP38259; MSMEI_1787.
DR GeneID; 66733267; -.
DR KEGG; msg:MSMEI_1787; -.
DR KEGG; msm:MSMEG_1830; -.
DR PATRIC; fig|246196.19.peg.1812; -.
DR eggNOG; COG0391; Bacteria.
DR OMA; DLDTVMY; -.
DR OrthoDB; 1079756at2; -.
DR UniPathway; UPA00071; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0043743; F:LPPG:FO 2-phospho-L-lactate transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR CDD; cd07186; CofD_like; 1.
DR Gene3D; 3.40.50.10680; -; 1.
DR HAMAP; MF_01257; CofD; 1.
DR InterPro; IPR002882; CofD.
DR InterPro; IPR038136; CofD-like_dom_sf.
DR InterPro; IPR010115; FbiA/CofD.
DR PANTHER; PTHR43007; PTHR43007; 1.
DR Pfam; PF01933; CofD; 1.
DR SUPFAM; SSF142338; SSF142338; 1.
DR TIGRFAMs; TIGR01819; F420_cofD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Magnesium; Reference proteome; Transferase.
FT CHAIN 1..327
FT /note="Phosphoenolpyruvate transferase"
FT /id="PRO_1000067251"
FT BINDING 59
FT /ligand="7,8-didemethyl-8-hydroxy-5-deazariboflavin"
FT /ligand_id="ChEBI:CHEBI:59904"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01257"
FT STRAND 2..7
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 10..23
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 26..28
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 36..41
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 56..65
FT /evidence="ECO:0007829|PDB:6UW5"
FT TURN 71..73
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 74..77
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 83..90
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 102..116
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 121..132
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 135..141
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 147..153
FT /evidence="ECO:0007829|PDB:6UW5"
FT TURN 155..157
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 160..164
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 165..169
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 178..184
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 186..188
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 195..200
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 203..207
FT /evidence="ECO:0007829|PDB:6UW5"
FT TURN 212..215
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 216..220
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 223..231
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 236..239
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 249..251
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 252..258
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 265..272
FT /evidence="ECO:0007829|PDB:6UW5"
FT TURN 275..278
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 281..287
FT /evidence="ECO:0007829|PDB:6UW5"
FT STRAND 298..302
FT /evidence="ECO:0007829|PDB:6UW5"
FT HELIX 309..323
FT /evidence="ECO:0007829|PDB:6UW5"
SQ SEQUENCE 327 AA; 34754 MW; 061F4C7BE305ACC2 CRC64;
MKITVLVGGV GGARFLLGVQ NLLGLGSFAD GPSKHELTAV VNIGDDAWMH GVRICPDLDT
CMYTLGGGID PDRGWGHRNE TWNAKEELAA YGVQPDWFGL GDRDLATHLV RSQMLRAGYP
LSQVTEALCK RWQPGARLLP ASDERSETHV VITDPTDGER RAIHFQEWWV RYRAKVPTHS
FAYVGADQAT AGPGVVEAIG DADIVLLAPS NPVVSIGPIL QIPGIRGALR STSAPVIGYS
PIIAGKPLRG MADECLKVIG VESTSQAVGE FFGARAGTGL LDGWLVHEGD HAQIEGVKVK
AVPLLMTDPE ATAAMVRAGL DLAGVSL
