FBL17_ARATH
ID FBL17_ARATH Reviewed; 593 AA.
AC Q8W104; Q9M1T4;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=F-box/LRR-repeat protein 17;
DE AltName: Full=F-box-like protein 17;
GN Name=FBL17; OrderedLocusNames=At3g54650; ORFNames=T5N23.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11077244; DOI=10.1016/s1360-1385(00)01769-6;
RA Xiao W., Jang J.-C.;
RT "F-box proteins in Arabidopsis.";
RL Trends Plant Sci. 5:454-457(2000).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP SUBCELLULAR LOCATION, AND INTERACTION WITH SKP1A/ASK1; KRP4; KRP6 AND KRP7.
RX PubMed=18948957; DOI=10.1038/nature07289;
RA Kim H.J., Oh S.A., Brownfield L., Hong S.H., Ryu H., Hwang I., Twell D.,
RA Nam H.G.;
RT "Control of plant germline proliferation by SCF(FBL17) degradation of cell
RT cycle inhibitors.";
RL Nature 455:1134-1137(2008).
CC -!- FUNCTION: Essential protein for male fertility. Component of the
CC SCF(ASK-cullin-F-box) E3 ubiquitin ligase complex SCF(FBL17), which
CC mediates the ubiquitination and subsequent proteasomal degradation of
CC target proteins. Enables the switch in cell cycle control leading to
CC male germ cell lineage formation from microspores after meiosis.
CC Targets CDKA-1 inhibitors the degradation specifically in male germ
CC cells (e.g. KRP6 and KRP7) and thus enables CDKA-1 activation and germ
CC cell S-phase progression. Promotes twin sperm cell production and
CC double fertilization. {ECO:0000269|PubMed:18948957}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex.
CC Interacts with SKP1A/ASK1, KRP4, KRP6 AND KRP7.
CC {ECO:0000269|PubMed:18948957}.
CC -!- INTERACTION:
CC Q8W104; Q0WNX9: KRP6; NbExp=3; IntAct=EBI-2026732, EBI-1253171;
CC Q8W104; Q94CL9: KRP7; NbExp=4; IntAct=EBI-2026732, EBI-1773344;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18948957}.
CC Note=Confined to the male germ cell nucleus of early to mid bicellular
CC pollen.
CC -!- TISSUE SPECIFICITY: Expressed in developing pollen.
CC {ECO:0000269|PubMed:18948957}.
CC -!- DEVELOPMENTAL STAGE: Expressed transiently in the male germ line after
CC asymmetric division. Present in maturing pollen with highest levels in
CC bicellular pollen. {ECO:0000269|PubMed:18948957}.
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC -!- DISRUPTION PHENOTYPE: Stabilization of KRP6 and inhibition of germ cell
CC cycle progression leading to male sterility.
CC {ECO:0000269|PubMed:18948957}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB77585.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL138650; CAB77585.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79261.1; -; Genomic_DNA.
DR EMBL; AF462827; AAL58916.1; -; mRNA.
DR EMBL; AY142006; AAM98270.1; -; mRNA.
DR PIR; T47624; T47624.
DR RefSeq; NP_567005.2; NM_115322.4.
DR AlphaFoldDB; Q8W104; -.
DR SMR; Q8W104; -.
DR BioGRID; 9946; 8.
DR DIP; DIP-46993N; -.
DR IntAct; Q8W104; 9.
DR STRING; 3702.AT3G54650.1; -.
DR iPTMnet; Q8W104; -.
DR PaxDb; Q8W104; -.
DR PRIDE; Q8W104; -.
DR ProteomicsDB; 222500; -.
DR EnsemblPlants; AT3G54650.1; AT3G54650.1; AT3G54650.
DR GeneID; 824630; -.
DR Gramene; AT3G54650.1; AT3G54650.1; AT3G54650.
DR KEGG; ath:AT3G54650; -.
DR Araport; AT3G54650; -.
DR TAIR; locus:2102400; AT3G54650.
DR eggNOG; ENOG502QR17; Eukaryota.
DR HOGENOM; CLU_019957_1_0_1; -.
DR InParanoid; Q8W104; -.
DR OMA; VHALGCQ; -.
DR OrthoDB; 633842at2759; -.
DR PhylomeDB; Q8W104; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q8W104; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8W104; baseline and differential.
DR Genevisible; Q8W104; AT.
DR GO; GO:0001673; C:male germ cell nucleus; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IDA:UniProtKB.
DR GO; GO:0035861; C:site of double-strand break; IDA:TAIR.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0055047; P:generative cell mitosis; IMP:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0051302; P:regulation of cell division; IMP:TAIR.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
DR GO; GO:0048316; P:seed development; IMP:TAIR.
DR Gene3D; 3.80.10.10; -; 3.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF00646; F-box; 1.
DR SMART; SM00367; LRR_CC; 5.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Developmental protein; Leucine-rich repeat; Nucleus;
KW Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..593
FT /note="F-box/LRR-repeat protein 17"
FT /id="PRO_0000272257"
FT DOMAIN 120..177
FT /note="F-box"
FT REPEAT 178..206
FT /note="LRR 1"
FT REPEAT 207..232
FT /note="LRR 2"
FT REPEAT 237..262
FT /note="LRR 3"
FT REPEAT 276..304
FT /note="LRR 4"
FT REPEAT 335..361
FT /note="LRR 5"
FT REPEAT 362..387
FT /note="LRR 6"
FT REPEAT 414..439
FT /note="LRR 7"
FT REPEAT 477..502
FT /note="LRR 8"
FT REPEAT 503..525
FT /note="LRR 9"
SQ SEQUENCE 593 AA; 64769 MW; B7C9668303455BB3 CRC64;
MQPQPHISPN TATAAISAAL ESQRSRKNRG SYNCGRCGQP KKGHVCLLTA PPDIPTTPIA
SEPVSCISAA ASSSRSTVLS LTAAPSSRQT FTHLRRALSF DDVDARNSLD ESDLDAASMD
LDLQLDTDIV QPGRFHAVGL WEVLKRLPPS SLLMAARVCK GWRETSRKMW KAAEELRIRV
PERAQIGYIG SLLQKCPRLI RLSLKIESDF DATTLACIAF SCPNLEVLEI TTSGAAVNRI
SGDELSRFVA NKRGLTSLKM EGCSNLGGFS LSSSSLSTLW LSDLHSLSKM IFNCPNLTEI
SLEFSRQEDD STDLVTMVDG LGRTCTRLQN IHIASLKLSH TVVLSLTAVN FRYLRMLSLV
LGINITDASV AAISSGYKNL ELLDLSGSSI TDTGLGMICD VLPDTLSKLL VALCPNITSS
GIQFATAQLP LLELMDCGMT VSDPNSDNPT FVENPSPHKT PGYNQKMFIK HKRLKKLSLW
GCSSLDALFL NCPELMDLNL NLCSNLHPES LVLQCPKLQL VYASGCQGLL TGAIRKQVSE
NFSAGENHMP RKRLADASKR IQALPSLYQE TREDGIYAGK RRKLEKEMCT IIH
