FBPA_HAEIN
ID FBPA_HAEIN Reviewed; 332 AA.
AC P35755; Q53439;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 3.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Iron-utilization periplasmic protein;
DE AltName: Full=Fe(3+)-binding protein;
DE AltName: Full=Iron-regulated 40 kDa protein;
DE AltName: Full=Major ferric iron-binding protein;
DE Short=MIRP;
DE Flags: Precursor;
GN Name=fbpA; Synonyms=fbp, hitA; OrderedLocusNames=HI_0097;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=NTHi TN106;
RX PubMed=7927717; DOI=10.1128/iai.62.10.4515-4525.1994;
RA Sanders J.D., Cope L.D., Hansen E.J.;
RT "Identification of a locus involved in the utilization of iron by
RT Haemophilus influenzae.";
RL Infect. Immun. 62:4515-4525(1994).
RN [3]
RP PROTEIN SEQUENCE OF 24-45.
RC STRAIN=Eagan / 8358;
RX PubMed=1556062; DOI=10.1128/jb.174.8.2425-2430.1992;
RA Harkness R.E., Chong P., Klein M.H.;
RT "Identification of two iron-repressed periplasmic proteins in Haemophilus
RT influenzae.";
RL J. Bacteriol. 174:2425-2430(1992).
RN [4]
RP PROTEIN SEQUENCE OF 24-31.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=10675023;
RX DOI=10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4;
RA Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C.,
RA Fountoulakis M.;
RT "Two-dimensional map of the proteome of Haemophilus influenzae.";
RL Electrophoresis 21:411-429(2000).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 24-332.
RX PubMed=9360608; DOI=10.1038/nsb1197-919;
RA Bruns C.M., Nowalk A.J., Arvai A.S., McTigue M.A., Vaughan K.G.,
RA Mietzner T.A., McRee D.E.;
RT "Structure of Haemophilus influenzae Fe(+3)-binding protein reveals
RT convergent evolution within a superfamily.";
RL Nat. Struct. Biol. 4:919-924(1997).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 24-332.
RX PubMed=11747438; DOI=10.1021/bi0156759;
RA Bruns C.M., Anderson D.S., Vaughan K.G., Williams P.A., Nowalk A.J.,
RA McRee D.E., Mietzner T.A.;
RT "Crystallographic and biochemical analyses of the metal-free Haemophilus
RT influenzae Fe(3+)-binding protein.";
RL Biochemistry 40:15631-15637(2001).
CC -!- FUNCTION: Part of the ABC transporter complex FbpABC (TC 3.A.1.10.1)
CC involved in Fe(3+) ions import. This protein specifically binds Fe(3+)
CC and is involved in its transmembrane transport (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (FbpC),
CC two transmembrane proteins (FbpB) and a solute-binding protein (FbpA).
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- INDUCTION: By iron deprivation.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 1 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC21773.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; L42023; AAC21773.1; ALT_FRAME; Genomic_DNA.
DR EMBL; S72674; AAB32110.1; -; Genomic_DNA.
DR PIR; A41833; A41833.
DR PIR; C64048; C64048.
DR PIR; T10886; T10886.
DR RefSeq; NP_438271.2; NC_000907.1.
DR PDB; 1D9V; X-ray; 1.75 A; A=24-332.
DR PDB; 1MRP; X-ray; 1.60 A; A=24-332.
DR PDB; 1NNF; X-ray; 1.10 A; A=24-332.
DR PDB; 1QVS; X-ray; 2.10 A; A=24-332.
DR PDB; 1QW0; X-ray; 1.90 A; A=24-332.
DR PDB; 2O68; X-ray; 1.70 A; A=24-332.
DR PDB; 2O69; X-ray; 2.00 A; A=24-332.
DR PDB; 2O6A; X-ray; 1.80 A; A=24-332.
DR PDB; 3KN7; X-ray; 1.71 A; A=24-332.
DR PDB; 3KN8; X-ray; 1.89 A; A=24-332.
DR PDB; 3OD7; X-ray; 1.80 A; A=24-332.
DR PDB; 3ODB; X-ray; 1.62 A; A=24-332.
DR PDBsum; 1D9V; -.
DR PDBsum; 1MRP; -.
DR PDBsum; 1NNF; -.
DR PDBsum; 1QVS; -.
DR PDBsum; 1QW0; -.
DR PDBsum; 2O68; -.
DR PDBsum; 2O69; -.
DR PDBsum; 2O6A; -.
DR PDBsum; 3KN7; -.
DR PDBsum; 3KN8; -.
DR PDBsum; 3OD7; -.
DR PDBsum; 3ODB; -.
DR AlphaFoldDB; P35755; -.
DR SMR; P35755; -.
DR STRING; 71421.HI_0097; -.
DR TCDB; 3.A.1.10.3; the atp-binding cassette (abc) superfamily.
DR PRIDE; P35755; -.
DR EnsemblBacteria; AAC21773; AAC21773; HI_0097.
DR KEGG; hin:HI_0097; -.
DR PATRIC; fig|71421.8.peg.100; -.
DR eggNOG; COG1840; Bacteria.
DR HOGENOM; CLU_026974_2_0_6; -.
DR PhylomeDB; P35755; -.
DR BioCyc; HINF71421:G1GJ1-102-MON; -.
DR EvolutionaryTrace; P35755; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR InterPro; IPR026045; Ferric-bd.
DR InterPro; IPR006059; SBP.
DR InterPro; IPR006061; SBP_1_CS.
DR Pfam; PF01547; SBP_bac_1; 1.
DR PIRSF; PIRSF002825; CfbpA; 1.
DR PROSITE; PS01037; SBP_BACTERIAL_1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Ion transport; Iron;
KW Iron transport; Metal-binding; Periplasm; Reference proteome; Signal;
KW Transport.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:10675023,
FT ECO:0000269|PubMed:1556062"
FT CHAIN 24..332
FT /note="Iron-utilization periplasmic protein"
FT /id="PRO_0000031703"
FT BINDING 32
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT BINDING 80
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT BINDING 218
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT BINDING 219
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT VARIANT 24
FT /note="D -> A (in strain: PAK12085 and SB33)"
FT VARIANT 37
FT /note="T -> A (in strain: TN106)"
FT VARIANT 37
FT /note="T -> V (in strain: PAK12085 and SB33)"
FT VARIANT 59
FT /note="E -> A (in strain: TN106)"
FT VARIANT 103
FT /note="Q -> K (in strain: TN106)"
FT VARIANT 119
FT /note="I -> V (in strain: TN106)"
FT VARIANT 284
FT /note="A -> V (in strain: TN106)"
FT VARIANT 323
FT /note="I -> T (in strain: TN106)"
FT STRAND 25..29
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 33..46
FT /evidence="ECO:0007829|PDB:1NNF"
FT STRAND 51..55
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 58..68
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 69..71
FT /evidence="ECO:0007829|PDB:1NNF"
FT STRAND 75..80
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 82..84
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 85..90
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 99..103
FT /evidence="ECO:0007829|PDB:1NNF"
FT STRAND 119..131
FT /evidence="ECO:0007829|PDB:1NNF"
FT TURN 132..134
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 137..139
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 144..148
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 150..152
FT /evidence="ECO:0007829|PDB:1NNF"
FT TURN 153..155
FT /evidence="ECO:0007829|PDB:1NNF"
FT STRAND 156..158
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 163..176
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 178..191
FT /evidence="ECO:0007829|PDB:1NNF"
FT STRAND 192..194
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 198..206
FT /evidence="ECO:0007829|PDB:1NNF"
FT STRAND 211..216
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 217..227
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 229..231
FT /evidence="ECO:0007829|PDB:1NNF"
FT STRAND 233..237
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 244..246
FT /evidence="ECO:0007829|PDB:1NNF"
FT STRAND 248..256
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 262..273
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 275..284
FT /evidence="ECO:0007829|PDB:1NNF"
FT STRAND 288..291
FT /evidence="ECO:0007829|PDB:3ODB"
FT HELIX 303..306
FT /evidence="ECO:0007829|PDB:1NNF"
FT HELIX 316..327
FT /evidence="ECO:0007829|PDB:1NNF"
SQ SEQUENCE 332 AA; 36193 MW; 4E6549FD76262CA6 CRC64;
MQFKHFKLAT LAAALAFSAN SFADITVYNG QHKEAATAVA KAFEQETGIK VTLNSGKSEQ
LAGQLKEEGD KTPADVFYTE QTATFADLSE AGLLAPISEQ TIQQTAQKGV PLAPKKDWIA
LSGRSRVVVY DHTKLSEKDM EKSVLDYATP KWKGKIGYVS TSGAFLEQVV ALSKMKGDKV
ALNWLKGLKE NGKLYAKNSV ALQAVENGEV PAALINNYYW YNLAKEKGVE NLKSRLYFVR
HQDPGALVSY SGAAVLKASK NQAEAQKFVD FLASKKGQEA LVAARAEYPL RADVVSPFNL
EPYEKLEAPV VSATTAQDKE HAIKLIEEAG LK
